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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||441 Antibodies||201 Antibodies||186 Antibodies|
|RNA Interference (RNAi)||2 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Immunoprecipitation (IP)||16 Antibodies||3 Antibodies||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||75 Antibodies||50 Antibodies||46 Antibodies|
|Immunohistochemistry (IHC)||198 Antibodies||82 Antibodies||72 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||132 Antibodies||121 Antibodies|
|Antigen||V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This RAF1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-RAF1 AntibodyTarget Details RAF1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of c-RAF protein. (region surrounding Met265)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details RAF1Product Details anti-RAF1 Antibody Application Details Handling Images back to top
|Alternative Name||RAF1 (RAF1 Antibody Abstract)|
|Background||The Raf family of serine/threonine-specific kinases is comprised of three members (A-Raf, B-Raf, and c-Raf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen-activated protein kinase (MAPK) pathway. c-Raf kinase (also known as Raf-1) is a key 74 kDa signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Activation of Raf kinase activity is induced by several events including phosphorylation at several sites and binding of GTP-bound Ras and/or 14-3-3 proteins. The c-Raf antibody is useful as a positive control and for measuring total c-Raf protein levels to compare signals obtained with the c-Raf PSSAs.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf|
|Molecular Weight||approx. 73 kDa|
|Research Area||Signaling, Kinases/Phosphatases|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-RAF1 Antibody Target Details RAF1 Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-RAF1 Antibody Target Details RAF1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling back to top