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anti-Rat (Rattus) RAF1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • c-raf
  • craf
  • raf1
  • RAF1
  • raf
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • AFR1
  • RAF
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • v-raf-1 murine leukemia viral oncogene homolog 1a
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • raf protein
  • v-raf-leukemia viral oncogene 1
  • zinc fingers and homeoboxes 2
  • raf1a
  • RAF1
  • raf1
  • LOC100341921
  • LOC100347407
  • c-raf
  • Raf1
  • ZHX2
  • Zhx2
Reactivity
Human, Mouse (Murine), Rat (Rattus)
798
339
323
18
18
10
5
4
4
2
2
2
1
1
Host
Rabbit
747
41
18
7
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
43
43
42
29
29
29
12
12
12
12
12
12
12
12
12
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
468
355
208
182
132
78
42
32
18
17
12
2
2
2
1
Supplier
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Supplier Product No.
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Catalog No. ABIN499021
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.203992 ABIN498893 IHC (p) WB Rabbit Log in to see Polyclonal
11.203992 ABIN498112 IHC (p) WB Rabbit Log in to see Polyclonal
11.203992 ABIN498113 IHC (p) WB Rabbit Log in to see Polyclonal
11.203992 ABIN498802 IHC (p) WB Rabbit pSer259 Log in to see Polyclonal
11.203992 ABIN712736 IF (p) IHC (p) WB Rabbit IgG AA 330-345, pSer339 Log in to see Polyclonal
11.203992 ABIN4349224 IHC IHC (p) WB Rabbit Log in to see Polyclonal
11.203992 ABIN4349220 IHC IHC (p) WB Rabbit Log in to see Polyclonal
11.203992 ABIN4349222 IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN733208 IF (p) IHC (p) WB Rabbit IgG AA 25-60 Log in to see Polyclonal 1
1 ABIN197102 IHC (p) WB Rabbit Ser259 Log in to see Polyclonal 6
1 ABIN271798 IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN271937 IF IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN302942 IHC (p) WB Rabbit IgG pSer259 Log in to see Polyclonal
1 ABIN318109 IHC (p) WB Rabbit pSer259 Log in to see Polyclonal
1 ABIN127014 IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal
1 ABIN127169 IHC (p) WB Rabbit pSer259 Log in to see Polyclonal
1 ABIN733628 IF (p) IHC (p) WB Rabbit IgG Tyr340, pSer338 Log in to see Polyclonal
1 ABIN733637 IHC (p) WB HRP Rabbit IgG Tyr340, pSer338 Log in to see Polyclonal
1 ABIN733630 IHC (p) WB Biotin Rabbit IgG Tyr340, pSer338 Log in to see Polyclonal
1 ABIN712745 IHC (p) WB HRP Rabbit IgG AA 330-345, pSer339 Log in to see Polyclonal

Top referenced anti-RAF1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-RAF1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Western Blotting (WB) 454 Antibodies 208 Antibodies 194 Antibodies
RNA Interference (RNAi) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 17 Antibodies 4 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 79 Antibodies 53 Antibodies 49 Antibodies
Immunohistochemistry (IHC) 207 Antibodies 87 Antibodies 77 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 132 Antibodies

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(798), (339), (323), (18), (18), (10), (5), (4), (4), (2), (2), (2), (1), (1)
Host Rabbit
(747), (41), (18), (7), (2)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(43), (43), (42), (29), (29), (29), (12), (12), (12), (12), (12), (12), (12), (12), (12)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(468), (355), (208), (182), (132), (78), (42), (32), (18), (17), (12), (2), (2), (2), (1)
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling Images
Specificity This antibody detects endogenous levels of Raf-1 protein. (region surroundign Arg282)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography
Purity > 95 % by SDS-PAGE

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name RAF1 (RAF1 Antibody Abstract)
Background Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-1 is activated in response to activation of a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. There is accumulating evidence that Ras p21 may play a role in activation of Raf-1 and may even play the role of the messenger from membrane tyrosine kinases to Raf-1.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
Molecular Weight approx. 73 kDa
Gene ID 5894
NCBI Accession NP_002871
UniProt P04049
Research Area Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling Images back to top
Application Notes Western Blot: 1/500 - 1/1000. Immunohistochemistry.1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (ABIN499021) Immunohistochemistry (IHC) analyzes of Raf-1 antibody in paraffin-embedded hum...
Western Blotting (WB) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (ABIN499021) Western blot (WB) analysis of Raf-1 antibody in extracts from hela c...