anti-Rat (Rattus) RAF1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • c-raf
  • craf
  • raf1
  • RAF1
  • raf
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • AFR1
  • RAF
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • v-raf-1 murine leukemia viral oncogene homolog 1a
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • raf protein
  • v-raf-leukemia viral oncogene 1
  • zinc fingers and homeoboxes 2
  • raf1a
  • RAF1
  • raf1
  • LOC100341921
  • LOC100347407
  • c-raf
  • Raf1
  • ZHX2
  • Zhx2
Epitope
AA 330-345, pSer339
80
73
47
47
46
43
41
34
33
28
27
21
21
19
18
17
15
15
15
15
14
12
7
7
7
6
6
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
835
412
387
25
23
16
16
13
8
8
8
5
4
4
2
2
1
1
1
Host
Rabbit
780
63
12
6
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
45
45
44
30
29
29
14
14
12
12
12
12
12
12
12
12
12
2
2
2
2
2
2
2
2
2
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
512
365
221
172
155
103
52
49
20
18
18
7
2
2
2
1
Options
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Catalog No. ABIN712736
$ 317.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.671247 ABIN214062 IHC (p) WB Rabbit IgG pSer259 Log in to see Polyclonal
13.671247 ABIN499021 IHC (p) WB Rabbit Log in to see Polyclonal
13.671247 ABIN498112 IHC (p) WB Rabbit Log in to see Polyclonal
13.671247 ABIN498113 IHC (p) WB Rabbit Log in to see Polyclonal
13.671247 ABIN498893 IHC (p) WB Rabbit Log in to see Polyclonal
13.671247 ABIN498802 IHC (p) WB Rabbit pSer259 Log in to see Polyclonal
13.671247 ABIN4349224 IHC IHC (p) WB Rabbit Log in to see Polyclonal
13.671247 ABIN4349222 IHC IHC (p) WB Rabbit Log in to see Polyclonal
13.671247 ABIN4349220 IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN733208 IF (p) IHC (p) WB Rabbit IgG AA 25-60 Log in to see Polyclonal 1
1 ABIN197102 IHC (p) WB Rabbit Ser259 Log in to see Polyclonal 6
1 ABIN733628 IF (p) IHC (p) WB Rabbit IgG Tyr340, pSer338 Log in to see Polyclonal
1 ABIN271798 IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN271937 IF IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN302942 IHC (p) WB Rabbit IgG pSer259 Log in to see Polyclonal
1 ABIN745343 IF (p) IHC (p) WB Rabbit IgG pSer289 Log in to see Polyclonal
1 ABIN2888517 IHC (p) ELISA WB Rabbit IgG pSer259 Log in to see Polyclonal
1 ABIN2888817 IHC (p) ELISA WB Rabbit IgG AA 11-60 Log in to see Polyclonal
1 ABIN2888728 IHC (p) ELISA WB Rabbit IgG AA 271-320 Log in to see Polyclonal
1 ABIN2889118 IHC (p) ELISA WB Rabbit IgG AA 251-300 Log in to see Polyclonal

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope AA 330-345, pSer339
(80), (73), (47), (47), (46), (43), (41), (34), (33), (28), (27), (21), (21), (19), (18), (17), (15), (15), (15), (15), (14), (12), (7), (7), (7), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(835), (412), (387), (25), (23), (16), (16), (13), (8), (8), (8), (5), (4), (4), (2), (2), (1), (1), (1)
Host Rabbit
(780), (63), (12), (6), (2)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(45), (45), (44), (30), (29), (29), (14), (14), (12), (12), (12), (12), (12), (12), (12), (12), (12), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(512), (365), (221), (172), (155), (103), (52), (49), (20), (18), (18), (7), (2), (2), (2), (1)
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic phosphopeptide derived from human c-Raf around the phosphorylation site of Ser339
Isotype IgG

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name C-Raf (RAF1 Antibody Abstract)
Background

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

Subcellular location: Mitochondrion

Synonyms: NS5, CRAF, Raf-1, c-Raf, CMD1NN, RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, RAF1, RAF

Gene ID 5894
UniProt P04049
Research Area Cancer
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 330-345), (pSer339) antibody (ABIN712736) Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-phospho-c-Raf(Ser...