anti-Rat (Rattus) RAF1 antibody for Immunoprecipitation

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • c-raf
  • craf
  • raf1
  • RAF1
  • raf
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • AFR1
  • RAF
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • v-raf-1 murine leukemia viral oncogene homolog 1a
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • raf protein
  • v-raf-leukemia viral oncogene 1
  • zinc fingers and homeoboxes 2
  • raf1a
  • RAF1
  • raf1
  • LOC100341921
  • LOC100347407
  • c-raf
  • Raf1
  • ZHX2
  • Zhx2
Epitope
C-Term
80
73
47
47
46
43
41
34
34
28
27
21
20
19
18
17
15
15
15
15
15
12
7
7
7
6
6
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
835
412
387
25
23
16
16
13
8
8
8
5
4
4
2
2
1
1
1
Host
Rabbit
780
63
12
6
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
45
45
44
30
29
29
14
14
12
12
12
12
12
12
12
12
12
2
2
2
2
2
2
2
2
2
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
512
365
220
172
156
104
52
49
20
18
17
7
2
2
2
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN222938
$ 265.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1003059 IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN127014 IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal
1 ABIN295906 IP WB Sheep IgG AA 657-668 Log in to see Polyclonal
1 ABIN4965583 IHC IP WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN209207 IP ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN233710 IP WB Rabbit IgG pSer259 Log in to see Polyclonal
1 ABIN233707 IP WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN241660 IP IHC WB Rabbit C-Term Log in to see Polyclonal
1 ABIN1929029 IP WB Rabbit IgG AA 598-648 Log in to see Polyclonal
1 ABIN229636 IF IP IHC WB Rabbit IgG Log in to see

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope C-Term
(80), (73), (47), (47), (46), (43), (41), (34), (34), (28), (27), (21), (20), (19), (18), (17), (15), (15), (15), (15), (15), (12), (7), (7), (7), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(835), (412), (387), (25), (23), (16), (16), (13), (8), (8), (8), (5), (4), (4), (2), (2), (1), (1), (1)
Host Rabbit
(780), (63), (12), (6), (2)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(45), (45), (44), (30), (29), (29), (14), (14), (12), (12), (12), (12), (12), (12), (12), (12), (12), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(512), (365), (220), (172), (156), (104), (52), (49), (20), (18), (17), (7), (2), (2), (2), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling References for anti-RAF1 antibody (ABIN222938) Images
Blocking Peptide Blocking peptide for this product available: ABIN412894
Specificity The antibody detects a 74 kDa Raf-1 protein on SDS-PAGE immunoblots.
Cross-Reactivity (Details) It is non cross-reactive with Raf-A and Raf-B.
Purification Affinity purified
Immunogen Synthetic peptide mapping to the C-terminus of human Raf-1
Isotype IgG

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling References for anti-RAF1 antibody (ABIN222938) Images back to top
Antigen
Alternative Name Raf1 (RAF1 Antibody Abstract)
Background Human Raf1 is a proto-oncogene-encoded 74 kDa kinase that serves as an upstream activator of Mek1 by phosphorylating Ser-218 and Ser-222 in the MAP kinase kinase (Mek1). It has also been implicated in the phosphorylation and activation of the cdc2 tyrosine phosphatase cdc25. Raf1 binds to the GTP-bound form of the Ras G-protein via an N-terminal domain in Raf-1. Raf-1 is activated upon phosphorylation by protein kinase C, Src and ceramide-activated protein kinase. Raf-1 is highly related to the A-Raf and B-Raf isoforms within the N-terminal G-protein binding domain (CR1), phosphorylation site domain (CR2) and the catalytic domain (CR3). Raf-1 physically interacts with Ras, RapIB, MEK1, the 14-3-3 family of proteins and the molecular chaperones hsp90 and p50.
Synonyms: RAF1, cRaf, c-Raf, RAF , CRAF , C-RAF
Gene ID 5894
UniProt P04049
Research Area Cancer, Apoptosis/Necrosis, Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling References for anti-RAF1 antibody (ABIN222938) Images back to top
Application Notes Western blot analysis (1-4 μg/mL), immunoprecipitation (15-25 μg/mL) and Immunohistochemistry (10-20 μg/mL). However, the optimal conditions should be determined individually. The antibody detects a 74 kDa Raf-1 protein on SDS-PAGE immunoblots. It is non cross-reactive with Raf-A and Raf-B.
Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details References for anti-RAF1 antibody (ABIN222938) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.2 mg/mL) immunoaffinity purified rabbit anti-Raf-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-RAF1 antibody (ABIN222938)

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
Product cited in:

Ito, Nagayasu, Okumura-Noji, Lu, Nishida, Miura, Asai, Kheirollah, Nakaya, Yokoyama: "Mechanism for FGF-1 to regulate biogenesis of apoE-HDL in astrocytes." in: Journal of lipid research, Vol. 48, Issue 9, pp. 2020-7, 2007

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling References for anti-RAF1 antibody (ABIN222938) back to top
Supplier Images
Western Blotting (WB) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (C-Term) antibody (ABIN222938) Western blot With Raf-l antibody, using rat kidney lysate (60 µg).