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anti-Rat (Rattus) RAF1 antibody for Western Blotting

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • c-raf
  • craf
  • raf1
  • RAF1
  • raf
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • AFR1
  • RAF
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • v-raf-1 murine leukemia viral oncogene homolog 1a
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • raf protein
  • v-raf-leukemia viral oncogene 1
  • zinc fingers and homeoboxes 2
  • raf1a
  • RAF1
  • raf1
  • LOC100341921
  • LOC100347407
  • c-raf
  • Raf1
  • ZHX2
  • Zhx2
Epitope
C-Term
75
71
49
39
37
36
29
29
28
28
23
22
22
21
20
19
17
15
15
15
15
15
14
14
11
9
9
8
8
6
6
6
6
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
819
425
401
26
25
17
16
13
9
7
7
5
4
3
2
2
1
1
1
1
Host
Rabbit
783
41
14
7
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
44
44
43
29
29
29
13
13
12
12
12
12
12
12
12
12
12
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
493
365
215
182
156
90
52
34
19
18
16
7
2
2
2
1
Options
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Catalog No. ABIN222938
$ 304.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0877867 ABIN1531571 ELISA WB Rabbit IgG pSer43 Log in to see Polyclonal 2
3.0877867 ABIN1531958 IHC ELISA WB Rabbit IgG pSer259 Log in to see Polyclonal 1
3.0877867 ABIN781705 EIA WB Chicken N-Term Log in to see Polyclonal 2
3.0877867 ABIN1532586 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
3.0877867 ABIN1532992 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
3.0877867 ABIN1531385 IHC ELISA WB Rabbit IgG pTyr341 Log in to see Polyclonal
3.0877867 ABIN1531386 IF IHC ELISA WB Rabbit IgG pSer621 Log in to see Polyclonal
3.0877867 ABIN1531428 IHC ELISA WB Rabbit IgG pSer296 Log in to see Polyclonal
3.0877867 ABIN1531959 ELISA WB Rabbit IgG pSer338 Log in to see Polyclonal
3.0877867 ABIN1870562 IHC WB Rabbit IgG pSer259 Log in to see Polyclonal
3.0877867 ABIN3186668 ELISA WB Rabbit IgG Thr560 Log in to see Polyclonal
3.0877867 ABIN3186672 ELISA IHC WB Rabbit IgG Thr58 Log in to see Polyclonal
3.0877867 ABIN3186674 ELISA IHC WB Rabbit IgG Thr596 Log in to see Polyclonal
3.0877867 ABIN3182138 ELISA IHC WB Rabbit IgG pSer259 Log in to see Polyclonal
3.0877867 ABIN3182575 ELISA IHC WB Rabbit IgG pSer296 Log in to see Polyclonal
3.0877867 ABIN3182404 ELISA WB Rabbit IgG pSer338 Log in to see Polyclonal
3.0877867 ABIN3182330 ELISA WB Rabbit IgG pSer301 Log in to see Polyclonal
3.0877867 ABIN3186667 ELISA IF IHC WB Rabbit IgG Thr56 Log in to see Polyclonal
3.0877867 ABIN3186669 ELISA IF WB Rabbit IgG Thr566 Log in to see Polyclonal
3.0877867 ABIN3186671 ELISA WB Rabbit IgG Thr573 Log in to see Polyclonal

Top referenced anti-RAF1 antibody for Western Blotting

Similar anti-RAF1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 1 Antibodies 1 Antibodies
ELISA 108 Antibodies 114 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 4 Antibodies
Immunofluorescence (IF) 25 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 143 Antibodies
Immunohistochemistry (IHC) 87 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 69 Antibodies
Immunoprecipitation (IP) 11 Antibodies
RNA Interference (RNAi) 1 Antibodies
Western Blotting (WB) 248 Antibodies
Flow Cytometry (FACS) 11 Antibodies

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope C-Term
(75), (71), (49), (39), (37), (36), (29), (29), (28), (28), (23), (22), (22), (21), (20), (19), (17), (15), (15), (15), (15), (15), (14), (14), (11), (9), (9), (8), (8), (6), (6), (6), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(819), (425), (401), (26), (25), (17), (16), (13), (9), (7), (7), (5), (4), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(783), (41), (14), (7), (2)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(44), (44), (43), (29), (29), (29), (13), (13), (12), (12), (12), (12), (12), (12), (12), (12), (12), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(493), (365), (215), (182), (156), (90), (52), (34), (19), (18), (16), (7), (2), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN222938) Images
Blocking Peptide Blocking peptide for this product available: ABIN412894
Specificity The antibody detects a 74 kDa Raf-1 protein on SDS-PAGE immunoblots.
Cross-Reactivity (Details) It is non cross-reactive with Raf-A and Raf-B.
Purification Affinity purified
Immunogen Synthetic peptide mapping to the C-terminus of human Raf-1
Isotype IgG

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling References for anti-RAF1 Antibody (ABIN222938) Images back to top
Antigen
Alternative Name Raf1 (RAF1 Antibody Abstract)
Background Human Raf1 is a proto-oncogene-encoded 74 kDa kinase that serves as an upstream activator of Mek1 by phosphorylating Ser-218 and Ser-222 in the MAP kinase kinase (Mek1). It has also been implicated in the phosphorylation and activation of the cdc2 tyrosine phosphatase cdc25. Raf1 binds to the GTP-bound form of the Ras G-protein via an N-terminal domain in Raf-1. Raf-1 is activated upon phosphorylation by protein kinase C, Src and ceramide-activated protein kinase. Raf-1 is highly related to the A-Raf and B-Raf isoforms within the N-terminal G-protein binding domain (CR1), phosphorylation site domain (CR2) and the catalytic domain (CR3). Raf-1 physically interacts with Ras, RapIB, MEK1, the 14-3-3 family of proteins and the molecular chaperones hsp90 and p50.
Synonyms: RAF1, cRaf, c-Raf, RAF , CRAF , C-RAF
Gene ID 5894
UniProt P04049
Research Area Cancer, Apoptosis/Necrosis, Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling References for anti-RAF1 Antibody (ABIN222938) Images back to top
Application Notes Western blot analysis (1-4 μg/mL), immunoprecipitation (15-25 μg/mL) and Immunohistochemistry (10-20 μg/mL). However, the optimal conditions should be determined individually. The antibody detects a 74 kDa Raf-1 protein on SDS-PAGE immunoblots. It is non cross-reactive with Raf-A and Raf-B.
Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details References for anti-RAF1 Antibody (ABIN222938) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.2 mg/mL) immunoaffinity purified rabbit anti-Raf-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-RAF1 Antibody (ABIN222938)

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
Product cited in:

Ito, Nagayasu, Okumura-Noji, Lu, Nishida, Miura, Asai, Kheirollah, Nakaya, Yokoyama: "Mechanism for FGF-1 to regulate biogenesis of apoE-HDL in astrocytes." in: Journal of lipid research, Vol. 48, Issue 9, pp. 2020-7, 2007

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN222938) back to top
Supplier Images
Western Blotting (WB) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (C-Term) (ABIN222938) Western blot With Raf-l antibody, using rat kidney lysate (60 µg).