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|Application / Reactivity||Human|
|Dot Blot (DB)||20 Antibodies|
|Enzyme Immunoassay (EIA)||7 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||10 Antibodies|
|Immunofluorescence (IF)||34 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||43 Antibodies|
|Immunohistochemistry (IHC)||88 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||46 Antibodies|
|Immunoprecipitation (IP)||39 Antibodies|
|Western Blotting (WB)||218 Antibodies|
|Antigen||Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) Antibodies|
|Conjugate||This RPS6KA1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA)
|5 references available|
|Supplier||Log in to see|
Product Details anti-RPS6KA1 AntibodyTarget Details RPS6KA1 Application Details Handling References for anti-RPS6KA1 Antibody (ABIN358393) Images
|Specificity||This antibody detects RPS6KA1 pSer363.|
|Purification||Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S363 of human RPS6KA1.|
Target Details RPS6KA1Product Details anti-RPS6KA1 Antibody Application Details Handling References for anti-RPS6KA1 Antibody (ABIN358393) Images back to top
|Alternative Name||RPS6KA1 (RPS6KA1 Antibody Abstract)|
|Background||RSK1 is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation.Synonyms: 90 kDa ribosomal protein S6 kinase 1, MAPKAPK1A, RSK-1, RSK1, Ribosomal S6 kinase 1, Ribosomal protein S6 kinase alpha-1, p90RSK1|
|Molecular Weight||82723 Da|
|Gene ID||6195, 9606|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Handling References for anti-RPS6KA1 Antibody (ABIN358393) Images back to top
ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details References for anti-RPS6KA1 Antibody (ABIN358393) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-RPS6KA1 Antibody (ABIN358393)Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details Handling Images back to top
Roux, Ballif, Anjum et al.: "Tumor-promoting phorbol esters and activated Ras inactivate the tuberous sclerosis tumor suppressor complex via p90 ribosomal S6 kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 37, pp. 13489-94, 2004 (PubMed).
Fernando, Wimalasena: "Estradiol abrogates apoptosis in breast cancer cells through inactivation of BAD: Ras-dependent nongenomic pathways requiring signaling through ERK and Akt." in: Molecular biology of the cell, Vol. 15, Issue 7, pp. 3266-84, 2004 (PubMed).
Hu, Fang, Dunham et al.: "90-kDa ribosomal S6 kinase is a direct target for the nuclear fibroblast growth factor receptor 1 (FGFR1): role in FGFR1 signaling." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 29325-35, 2004 (PubMed).
Bohuslav, Chen, Kwon et al.: "p53 induces NF-kappaB activation by an IkappaB kinase-independent mechanism involving phosphorylation of p65 by ribosomal S6 kinase 1." in: The Journal of biological chemistry, Vol. 279, Issue 25, pp. 26115-25, 2004 (PubMed).
Cavet, Lehoux, Berk: "14-3-3beta is a p90 ribosomal S6 kinase (RSK) isoform 1-binding protein that negatively regulates RSK kinase activity." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 18376-83, 2003 (PubMed).
ImagesProduct Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details Handling References for anti-RPS6KA1 Antibody (ABIN358393) back to top