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anti-Human RPS6KA1 antibody for Enzyme Immunoassay

Recommended RPS6KA1 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) Antibodies
  • CLS
  • hu-1
  • HU-1
  • HU-3
  • ISPK-1
  • mapkapk1a
  • MAPKAPK1A
  • MAPKAPK1B
  • MRX19
  • p90-RSK2
  • p90rsk
  • pp90RSK2
  • RPS6KA
  • rsk
  • RSK
  • RSK1
  • rsk1
  • Rsk1
  • RSK2
  • S6K-alpha3
Epitope
pSer363
67
39
32
24
22
18
15
12
10
10
9
8
7
6
6
4
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
316
146
116
29
11
7
6
2
2
1
Host
Rabbit
314
39
Clonality
Polyclonal
Conjugate
This RPS6KA1 antibody is un-conjugated
20
19
17
12
12
12
5
5
5
5
5
5
5
5
4
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA)
249
157
98
54
53
40
35
20
11
6
2
1
1
Supplier
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Catalog No. ABIN358393
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN401473 EIA WB Rabbit IgG C-Term, pSer732 Log in to see Polyclonal 1
0.0008867729 ABIN264872 EIA IP WB Rabbit IgG C-Term Log in to see Polyclonal 4
0.0008867729 ABIN954622 EIA IHC (p) WB Rabbit Ig Log in to see Polyclonal 1
0.0008867729 ABIN500636 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008867729 ABIN1108902 EIA IHC (p) WB Mouse IgG2a Log in to see 10B1D7A9 3
0.0008867729 ABIN401354 EIA IP WB Rabbit IgG C-Term Log in to see Polyclonal

Top referenced anti-RPS6KA1 antibody for Enzyme Immunoassay

Similar anti-RPS6KA1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 20 Antibodies
ELISA 142 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 13 Antibodies
Immunofluorescence (IF) 37 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 43 Antibodies
Immunohistochemistry (IHC) 92 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 50 Antibodies
Immunoprecipitation (IP) 40 Antibodies
Western Blotting (WB) 224 Antibodies

General

Antigen Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) Antibodies
Epitope pSer363
(67), (39), (32), (24), (22), (18), (15), (12), (10), (10), (9), (8), (7), (6), (6), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(316), (146), (116), (29), (11), (7), (6), (2), (2), (1)
Host Rabbit
(314), (39)
Clonality
Polyclonal
Conjugate This RPS6KA1 antibody is un-conjugated
(20), (19), (17), (12), (12), (12), (5), (5), (5), (5), (5), (5), (5), (5), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA)
(249), (157), (98), (54), (53), (40), (35), (20), (11), (6), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-RPS6KA1 Antibody

Target Details RPS6KA1 Application Details Handling References for anti-RPS6KA1 Antibody (ABIN358393) Images
Specificity This antibody detects RPS6KA1 pSer363.
Purification Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S363 of human RPS6KA1.
Isotype Ig

Target Details RPS6KA1

Product Details anti-RPS6KA1 Antibody Application Details Handling References for anti-RPS6KA1 Antibody (ABIN358393) Images back to top
Antigen
Alternative Name RPS6KA1 (RPS6KA1 Antibody Abstract)
Background RSK1 is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation.Synonyms: 90 kDa ribosomal protein S6 kinase 1, MAPKAPK1A, RSK-1, RSK1, Ribosomal S6 kinase 1, Ribosomal protein S6 kinase alpha-1, p90RSK1
Molecular Weight 82723 Da
Gene ID 6195, 9606
UniProt Q15418
Research Area Signaling
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Handling References for anti-RPS6KA1 Antibody (ABIN358393) Images back to top
Application Notes ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details References for anti-RPS6KA1 Antibody (ABIN358393) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-RPS6KA1 Antibody (ABIN358393)

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details Handling Images back to top
Background publications

Roux, Ballif, Anjum, Gygi, Blenis: "Tumor-promoting phorbol esters and activated Ras inactivate the tuberous sclerosis tumor suppressor complex via p90 ribosomal S6 kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 37, pp. 13489-94, 2004

Fernando, Wimalasena: "Estradiol abrogates apoptosis in breast cancer cells through inactivation of BAD: Ras-dependent nongenomic pathways requiring signaling through ERK and Akt." in: Molecular biology of the cell, Vol. 15, Issue 7, pp. 3266-84, 2004

Bohuslav, Chen, Kwon, Mu, Greene: "p53 induces NF-kappaB activation by an IkappaB kinase-independent mechanism involving phosphorylation of p65 by ribosomal S6 kinase 1." in: The Journal of biological chemistry, Vol. 279, Issue 25, pp. 26115-25, 2004

Hu, Fang, Dunham, Prada, Stachowiak, Stachowiak: "90-kDa ribosomal S6 kinase is a direct target for the nuclear fibroblast growth factor receptor 1 (FGFR1): role in FGFR1 signaling." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 29325-35, 2004

Cavet, Lehoux, Berk: "14-3-3beta is a p90 ribosomal S6 kinase (RSK) isoform 1-binding protein that negatively regulates RSK kinase activity." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 18376-83, 2003

Images

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details Handling References for anti-RPS6KA1 Antibody (ABIN358393) back to top
Supplier Images
 image for anti-RPS6KA1 antibody (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1) (pSer363) (ABIN358393) anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (pSer363) antibody