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anti-Mouse (Murine) RPS6KA1 antibody for Flow Cytometry

Recommended RPS6KA1 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) Antibodies
  • CLS
  • hu-1
  • HU-1
  • HU-3
  • ISPK-1
  • mapkapk1a
  • MAPKAPK1A
  • MAPKAPK1B
  • MRX19
  • p90-RSK2
  • p90rsk
  • pp90RSK2
  • RPS6KA
  • rsk
  • RSK
  • RSK1
  • rsk1
  • Rsk1
  • RSK2
  • S6K-alpha3
Epitope
N-Term
67
40
32
24
22
18
15
12
10
10
9
8
7
6
6
4
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
317
146
116
29
11
7
6
2
2
1
Host
Rabbit
315
39
Clonality
Monoclonal
Conjugate
This RPS6KA1 antibody is un-conjugated
20
19
17
12
12
12
5
5
5
5
5
5
5
5
4
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
249
157
98
54
53
40
35
20
11
7
2
1
1
Supplier
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Available images

Catalog No. ABIN258452
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Similar anti-RPS6KA1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 39 Antibodies 55 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 3 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 3 Antibodies
Immunofluorescence (IF) 16 Antibodies 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies 44 Antibodies
Immunohistochemistry (IHC) 22 Antibodies 24 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies 22 Antibodies
Immunoprecipitation (IP) 26 Antibodies 25 Antibodies
Western Blotting (WB) 76 Antibodies

General

Antigen Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) Antibodies
Epitope N-Term
(67), (40), (32), (24), (22), (18), (15), (12), (10), (10), (9), (8), (7), (6), (6), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(317), (146), (116), (29), (11), (7), (6), (2), (2), (1)
Host Rabbit
(315), (39)
Clonality (Clone)
Monoclonal   ( )
Conjugate This RPS6KA1 antibody is un-conjugated
(20), (19), (17), (12), (12), (12), (5), (5), (5), (5), (5), (5), (5), (5), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(249), (157), (98), (54), (53), (40), (35), (20), (11), (7), (2), (1), (1)
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Product Details anti-RPS6KA1 Antibody

Target Details RPS6KA1 Application Details Handling Images
Purification IgG purified
Immunogen A synthetic peptide corresponding to residues in N-terminus of human p90-RSK1 was used as immunogen.
Clone E4
Isotype IgG

Target Details RPS6KA1

Product Details anti-RPS6KA1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name RPS6KA1 (RPS6KA1 Antibody Abstract)
Background Rsk-1 (p90-Rsk-1) is a member of a family of 90 kDa ribosomal protein S6 kinases,which includes Rsk-1, Rsk-2 and Rsk-3. These are broadly expressed serine/threonineprotein kinases (1) activated in response to mitogenic stimuli, including extracellularsignal-regulated protein kinases Erk1 and Erk2 (2). p90-Rsk-1 is activated by MAPK invitro and in vivo via phosphorylation (3). Active Rsks appear to play a major role intranscriptional regulation by translocating to the nucleus and phosphorylating c-Fos andCREB (2,4). Alternate Names: anti-90 kDa ribosomal protein S6 kinase 1 antibody, anti-dJ590P13.1 antibody, anti-HU1 antibody, anti-HU1 antibody, anti-MAP kinase activated protein kinase 1a antibody,anti-MAPKAPK1A antibody, anti-p90 RSK1 antibody, anti-p90S6K antibody,anti-pp90RSK1 antibody, anti-Ribosomal protein S6 kinase 90kD 1 antibody,anti-Ribosomal protein S6 kinase 90kD polypeptide 1 antibody, anti-Ribosomal proteinS6 kinase 90kDa polypeptide 1 antibody, anti-Ribosomal protein S6 kinase alpha 1antibody, anti-Ribosomal S6 kinase 1 antibody, anti-Rps6ka1 antibody, anti-RSK 1antibody, anti-RSK 1 p90 antibody, anti-RSK antibody, anti-RSK1 antibody, anti-S6Kalpha 1 antibody.
Gene Symbol: RPS6KA1
Gene ID 6195, 20111, 81771
Research Area Signaling
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 83kDa.
Recommended dilutions: Flow Cytometry 1:50, Immunocytochemistry/Immunofluorescence 1:200, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:100, Immunoprecipitation 1:10, Western Blot 1:1000
Protocol Protocol specific for RSK1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-RPS6KA1 antibody (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1) (N-Term) (ABIN258452) Western blot. EGF treated A431 cell line lysate probed with anti-RSK1.
Immunohistochemistry (IHC) image for anti-RPS6KA1 antibody (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1) (N-Term) (ABIN258452) IHC ? Colon tissue section stained for RSK-1 subcellular location using anti-RSK-1.
Flow Cytometry (FACS) image for anti-RPS6KA1 antibody (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1) (N-Term) (ABIN258452) Flow cytometric analysis of permeabilized Jurkat cells using anti-Rsk-1 RabMAb (red) ...