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anti-Rat (Rattus) RPS6KA1 antibody for Immunofluorescence

Recommended RPS6KA1 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) Antibodies
  • CLS
  • hu-1
  • HU-1
  • HU-3
  • ISPK-1
  • mapkapk1a
  • MAPKAPK1A
  • MAPKAPK1B
  • MRX19
  • p90-RSK2
  • p90rsk
  • pp90RSK2
  • RPS6KA
  • rsk
  • RSK
  • RSK1
  • rsk1
  • Rsk1
  • RSK2
  • S6K-alpha3
Epitope
AA 1-184
64
40
32
24
22
18
15
12
10
9
9
8
7
6
6
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
308
141
111
28
10
6
5
2
2
1
Host
Mouse
308
37
Clonality
Monoclonal
Conjugate
This RPS6KA1 antibody is un-conjugated
20
19
17
12
12
12
5
5
5
5
5
5
5
5
4
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
240
154
92
54
48
38
30
20
7
7
1
1
1
Supplier
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Catalog No. ABIN967837
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3140218 ABIN967838 IF IP IHC WB Mouse IgG2a AA 1-184 Log in to see 78-RSK 5
0.3140218 ABIN1870594 IF IHC WB Rabbit IgG pThr348 Log in to see Polyclonal
0.3140218 ABIN2505003 IF IHC ELISA WB Rabbit IgG Thr573 Log in to see Polyclonal
0.3140218 ABIN2505004 IF IHC ELISA WB Rabbit IgG pThr573 Log in to see Polyclonal
0.3140218 ABIN2424865 IF IHC WB Rabbit IgG pThr348 Log in to see Polyclonal
0.0010908465 ABIN197328 IF WB Rabbit Thr348 Log in to see Polyclonal 3
0.0010908465 ABIN196796 IF IHC (p) WB Rabbit pThr348 Log in to see Polyclonal 3
0.0010908465 ABIN498221 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0010908465 ABIN258452 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see E4
0.0010908465 ABIN446802 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal
0.0010908465 ABIN3182764 ELISA IF IHC WB Rabbit IgG pThr573 Log in to see Polyclonal
0.0010908465 ABIN3186844 ELISA IF IHC WB Rabbit IgG Thr853 Log in to see Polyclonal
0.0010908465 ABIN2425163 IF WB Rabbit IgG Log in to see Polyclonal
0.0010908465 ABIN2987490 ICC IF IHC WB Rabbit IgG pThr348 Log in to see Polyclonal

Top referenced anti-RPS6KA1 antibody for Immunofluorescence

Similar anti-RPS6KA1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Chicken
Western Blotting (WB) 218 Antibodies 101 Antibodies 74 Antibodies 10 Antibodies 5 Antibodies
Immunoprecipitation (IP) 39 Antibodies 24 Antibodies 25 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 46 Antibodies 20 Antibodies 19 Antibodies
Immunohistochemistry (IHC) 88 Antibodies 22 Antibodies 19 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies

General

Antigen Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) Antibodies
Epitope AA 1-184
(64), (40), (32), (24), (22), (18), (15), (12), (10), (9), (9), (8), (7), (6), (6), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(308), (141), (111), (28), (10), (6), (5), (2), (2), (1)
Host Mouse
(308), (37)
Clonality (Clone)
Monoclonal   ( )
Conjugate This RPS6KA1 antibody is un-conjugated
(20), (19), (17), (12), (12), (12), (5), (5), (5), (5), (5), (5), (5), (5), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(240), (154), (92), (54), (48), (38), (30), (20), (7), (7), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-RPS6KA1 Antibody

Target Details RPS6KA1 Application Details Handling References for anti-RPS6KA1 Antibody (ABIN967837) Images
Cross-Reactivity Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human p90[rsk] aa. 1-184
Clone 78-RSK
Isotype IgG2a

Target Details RPS6KA1

Product Details anti-RPS6KA1 Antibody Application Details Handling References for anti-RPS6KA1 Antibody (ABIN967837) Images back to top
Antigen
Alternative Name Rsk (RPS6KA1 Antibody Abstract)
Background The p90[rsk] (Rsk) and p70[s6k] kinases were first identified based on their ability to phosphorylate the 40S ribosomal protein S6 in vitro. Both of these enzymes are differentially regulated by serine/threonine phosphorylation in response to mitogenic stimulation. ERK1 and ERK2 have been shown to regulate Rsk activity. Once activated by this phosphorylation, a significant amount of Rsk can be found in the nucleus, suggesting that it has a role in nuclear signaling events. The regulation of nuclear Rsk and ERK activities by growth factors is coordinated with the induction of several early response genes. Rsk has also been shown to be activated by ionizing radiation, presumably through an activated MAP kinase. Studies in Xenopus oocytes and mouse NIH/3T3 cells indicate that inactive Rsk and ERK2 exist in a complex of approximately 110kDa. Upon phosphorylation of Rsk and ERK2, the heterodimer dissociates and at least a portion of these activated kinases translocate to the nucleus.
Molecular Weight 90 kDa
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Handling References for anti-RPS6KA1 Antibody (ABIN967837) Images back to top
Comment

Related Products: ABIN967389, ABIN968533

Restrictions For Research Use only

Handling

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details References for anti-RPS6KA1 Antibody (ABIN967837) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-RPS6KA1 Antibody (ABIN967837)

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details Handling Images back to top
Product cited in:

Moor, Gan, Karmazyn, Fliegel: "Activation of Na+/H+ exchanger-directed protein kinases in the ischemic and ischemic-reperfused rat myocardium." in: The Journal of biological chemistry, Vol. 276, Issue 19, pp. 16113-22, 2001

Morrione, Navarro, Romano, Dews, Reiss, Valentinis, Belletti, Baserga: "The role of the insulin receptor substrate-1 in the differentiation of rat hippocampal neuronal cells." in: Oncogene, Vol. 20, Issue 35, pp. 4842-52, 2001

Majka, Janowska-Wieczorek, Ratajczak, Kowalska, Vilaire, Pan, Honczarenko, Marquez, Poncz, Ratajczak: "Stromal-derived factor 1 and thrombopoietin regulate distinct aspects of human megakaryopoiesis." in: Blood, Vol. 96, Issue 13, pp. 4142-51, 2000

Hsiao, Chou, Shih, Ferrell: "Evidence that inactive p42 mitogen-activated protein kinase and inactive Rsk exist as a heterodimer in vivo." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 12, pp. 5480-4, 1994

Chung, Pelech, Blenis: "Mitogen-activated Swiss mouse 3T3 RSK kinases I and II are related to pp44mpk from sea star oocytes and participate in the regulation of pp90rsk activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 11, pp. 4981-5, 1991

Images

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details Handling References for anti-RPS6KA1 Antibody (ABIN967837) back to top
Supplier Images
Western Blotting (WB) image for anti-RPS6KA1 antibody (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1) (AA 1-184) (ABIN967837) Western blot analysis of Rsk on A431 cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lan...
Immunofluorescence (IF) image for anti-RPS6KA1 antibody (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1) (AA 1-184) (ABIN967837) Immunofluorescent staining of Hs 766T cells.