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Human RPS6KA1 ELISA Kit

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Antigen
Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) ELISA Kits
  • CLS
  • hu-1
  • HU-1
  • HU-3
  • ISPK-1
  • mapkapk1a
  • MAPKAPK1A
  • MAPKAPK1B
  • MRX19
  • p90-RSK2
  • p90rsk
  • pp90RSK2
  • RPS6KA
  • rsk
  • RSK
  • RSK1
  • rsk1
  • Rsk1
  • RSK2
  • S6K-alpha3
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
7
1
1
Detection Range
0.31-20 ng/mL
Minimum Detection Limit
0.31 ng/mL
Application
ELISA
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Catalog No. ABIN1450246
$ 810.53
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.0012117105 ABIN2748547 Sandwich ELISA Plasma, Serum, Cell Culture Supernatant, Cell and Tissue Culture Lysate, Body Fluids Log in to see
0.0012117105 ABIN2748624 Sandwich ELISA Cell Lysate Log in to see
0.0012117105 ABIN2851505 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.313-20 ng/mL 0.313 ng/mL Log in to see
0.0012117105 ABIN2748625 Cell ELISA Log in to see
0.0012117105 ABIN2685392 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see
0.0012117105 ABIN2044471 Sandwich ELISA 0.31-20 ng/mL 0.31 ng/mL Log in to see
0.0012117105 ABIN2538684 Sandwich ELISA Tissue Homogenate, Cell Lysate, Biological Fluids 0.313-20 ng/mL 0.313 ng/mL Log in to see

Similar RPS6KA1 ELISA Kits

Application / Reactivity Human
ELISA 8 ELISA Kits

General

Antigen Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(7), (1), (1)
Detection Range 0.31-20 ng/mL
Minimum Detection Limit 0.31 ng/mL
Application ELISA
Supplier Log in to see

Product Details RPS6KA1 ELISA Kit

Target details Application Details Handling Images
Sample Type Tissue Homogenate, Cell Lysate, Biological Fluids
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1).
Cross-Reactivity (Details) No significant cross-reactivity or interference between Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1) and analogues was observed.
Sensitivity 0.117 ng/mL
Components
  • Pre-coated, ready to use 96-well strip plate - 1
  • Plate sealer for 96 wells - 4
  • Standard (lyophilized) - 2
  • Standard Diluent - 1×20mL
  • Detection Reagent A (green) - 1×120µL
  • Assay Diluent A (2 × concentrate) - 1×6mL
  • Detection Reagent B (red) - 1×120µL
  • Assay Diluent B (2 × concentrate) - 1×6mL
  • TMB Substrate - 1×9mL
  • Stop Solution - 1×6mL
  • Wash Buffer (30 × concentrate) - 1×20mL
  • Instruction manual - 1
Material not included
  • Microplate reader with 450nm filter.
  • Precision single or multi-channel pipettes and disposable tips.
  • Eppendorf Tubes for diluting samples.
  • Deionized or distilled water.
  • Absorbent paper for blotting the microtiter plate.
  • Container for Wash Solution

Target details

Product Details RPS6KA1 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1) (RPS6KA1 ELISA Kit Abstract)
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details RPS6KA1 ELISA Kit Target details Handling Images back to top
Application Notes
  • Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
  • The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
  • Kits from different batches may be a little different in detection range, sensitivity and color developing time.
  • Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
  • Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
  • There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
  • Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
  • Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
  • Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
  • Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.
Comment

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Sample Volume 100 μL
Assay Time 4.5 h
Plate Pre-coated,Strips (12 x 8)
Assay Precision Intra-assay Precision (precision within an assay): Three samples with low, medium and high levels of the target antigen were tested twenty times on one plate, respectively.
Inter-assay Precision (precision between assays): Three samples with low, medium and high levels of the target antigen were tested on three different plates, eight replicates in each plate.
CV (%) = SD/mean X 100
  • Intra-assay: CV less than 10 %
  • Inter-assay: CV less than 12 %
Restrictions For Research Use only

Handling

Product Details RPS6KA1 ELISA Kit Target details Application Details Images back to top
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Storage 4 °C/-20 °C
Storage Comment
  • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon receipt while the others should be at 0 °C.
  • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
    Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
  • For ELISA kit, 1 day storage at 30 °C can be considered as 2 months at 0 °C, which means 3 days at 30 °C equaling 6 months at 0 °C.
Expiry Date 6 months

Images

Product Details RPS6KA1 ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for RPS6KA1 ELISA Kit (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1) (ABIN1450246) Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) ELISA Kit