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|Antigen||Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) ELISA Kits|
Kits with alternative reactivity to:
|Method Type||Sandwich ELISA|
|Detection Range||0.31-20 ng/mL|
|Minimum Detection Limit||0.31 ng/mL|
|Supplier||Log in to see|
Product Details RPS6KA1 ELISA KitTarget details Application Details Handling Images
|Sample Type||Tissue Homogenate, Cell Lysate, Biological Fluids|
This assay has high sensitivity and excellent specificity for detection of Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1).
|Cross-Reactivity (Details)||No significant cross-reactivity or interference between Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1) and analogues was observed.|
|Material not included||
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|Alternative Name||Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1) (RPS6KA1 ELISA Kit Abstract)|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades|
Application DetailsProduct Details RPS6KA1 ELISA Kit Target details Handling Images back to top
Information on standard material:
|Sample Volume||100 μL|
|Assay Time||3 h|
|Protocol||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.|
1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 2 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1) were tested 20 times on one plate, respectively.
|Restrictions||For Research Use only|
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|Precaution of Use||The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.|
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
|Expiry Date||6 months|
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