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Human RPS6KA1 ELISA Kit

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Antigen
Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) ELISA Kits
  • Rsk1
  • p90rsk
  • rsk
  • HU-1
  • MAPKAPK1A
  • RSK
  • RSK1
  • RPS6KA
  • hu-1
  • mapkapk1a
  • rsk1
  • CLS
  • HU-3
  • ISPK-1
  • MAPKAPK1B
  • MRX19
  • RSK2
  • S6K-alpha3
  • p90-RSK2
  • pp90RSK2
  • ribosomal protein S6 kinase polypeptide 1
  • ribosomal protein S6 kinase, 90kDa, polypeptide 1
  • ribosomal protein S6 kinase, 90kDa, polypeptide 3
  • Rps6ka1
  • RPS6KA1
  • rps6ka1
  • RPS6KA3
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
8
1
1
Method Type
Sandwich ELISA
Detection Range
0.31-20 ng/mL
Minimum Detection Limit
0.31 ng/mL
Application
ELISA
Options
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Catalog No. ABIN1450246
$ 648.42
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN2851505 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.312-20 ng/mL 0.312 ng/mL Log in to see
1 ABIN2748547 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
1 ABIN2748625 Cell ELISA Cell Culture Cells Log in to see
1 ABIN2748624 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
1 ABIN2685392 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see
1 ABIN2538684 Sandwich ELISA Tissue Homogenate, Cell Lysate, Biological Fluids 0.313-20 ng/mL 0.313 ng/mL Log in to see
1 ABIN4972580 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 0.312-20 ng/mL 0.312 ng/mL Log in to see
1 ABIN2044471 Sandwich ELISA 0.31-20 ng/mL 0.31 ng/mL Log in to see

Similar RPS6KA1 ELISA Kits

Application / Reactivity Human
ELISA 9 ELISA Kits

General

Antigen Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(8), (1), (1)
Method Type Sandwich ELISA
Detection Range 0.31-20 ng/mL
Minimum Detection Limit 0.31 ng/mL
Application ELISA
Supplier Log in to see

Product Details RPS6KA1 ELISA Kit

Target details Application Details Handling Images
Sample Type Tissue Homogenate, Cell Lysate, Biological Fluids
Detection Method Colorimetric
Specificity

This assay has high sensitivity and excellent specificity for detection of Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1).
No significant cross-reactivity or interference between Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1) and analogues was observed.

Cross-Reactivity (Details) No significant cross-reactivity or interference between Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1) and analogues was observed.
Sensitivity 0.117 ng/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Plate sealer for 96 wells
  • Standard Diluent
  • Assay Diluent A
  • Assay Diluent B
  • Stop Solution
  • Standard
  • Detection Reagent A
  • Detection Reagent B
  • Substrate A
  • Wash Buffer (30 × concentrate)
  • Instruction manual
Material not included
  • Microplate reader with 450 nm filter.
  • Precision single or multi-channel pipettes and disposable tips.
  • Eppendorf Tubes for diluting samples.
  • Deionized or distilled water.
  • Absorbent paper for blotting the microtiter plate.
  • Container for Wash Solution

Target details

Product Details RPS6KA1 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1) (RPS6KA1 ELISA Kit Abstract)
UniProt Q15418
Pathways MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades

Application Details

Product Details RPS6KA1 ELISA Kit Target details Handling Images back to top
Application Notes
  • Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
  • The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
  • Kits from different batches may be a little different in detection range, sensitivity and color developing time.
  • Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
  • Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
  • There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
  • Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
  • Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
  • Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
  • Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.
Comment

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Sample Volume 100 μL
Assay Time 3 h
Plate Pre-coated,Strips (12 x 8)
Protocol The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Procedure 1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 2 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.
Assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions For Research Use only

Handling

Product Details RPS6KA1 ELISA Kit Target details Application Details Images back to top
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Storage 4 °C
Storage Comment
  • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon receipt while the others should be at 0 °C.
  • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
    Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
  • For ELISA kit, 1 day storage at 30 °C can be considered as 2 months at 0 °C, which means 3 days at 30 °C equaling 6 months at 0 °C.
Expiry Date 6 months

Images

Product Details RPS6KA1 ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for RPS6KA1 ELISA Kit (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1) (ABIN1450246) Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) ELISA Kit