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anti-Human RPS6KA3 antibody for Enzyme Immunoassay

Recommended RPS6KA3 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3) Antibodies
  • CLS
  • HU-3
  • ISPK-1
  • MAPKAPK1B
  • MPK-9
  • MRX19
  • p90-RSK2
  • pp90RSK2
  • RSK
  • Rsk2
  • RSK2
  • S6K-alpha3
Epitope
N-Term
21
14
13
13
12
7
6
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
126
53
47
6
6
5
3
3
2
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
114
24
Clonality
Polyclonal
Conjugate
This RPS6KA3 antibody is un-conjugated
6
6
6
4
4
4
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
90
43
24
22
21
18
11
7
5
4
2
2
2
Supplier
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Catalog No. ABIN360345
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008871923 ABIN358394 EIA Rabbit Ig pSer369 Log in to see Polyclonal 5
0.0008871923 ABIN1108903 EIA IF WB Mouse IgG1 Log in to see 4-00E-010 1

Top referenced anti-RPS6KA3 antibody for Enzyme Immunoassay

Similar anti-RPS6KA3 Antibodies

Application / Reactivity Human
Dot Blot (DB) 18 Antibodies
ELISA 43 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 24 Antibodies
Immunohistochemistry (IHC) 20 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 80 Antibodies

General

Antigen Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3) Antibodies
Epitope N-Term
(21), (14), (13), (13), (12), (7), (6), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(126), (53), (47), (6), (6), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(114), (24)
Clonality
Polyclonal
Conjugate This RPS6KA3 antibody is un-conjugated
(6), (6), (6), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(90), (43), (24), (22), (21), (18), (11), (7), (5), (4), (2), (2), (2)
Pubmed 5 references available
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Product Details anti-RPS6KA3 Antibody

Target Details RPS6KA3 Application Details Handling References for anti-RPS6KA3 Antibody (ABIN360345) Images
Specificity This antibody reacts to RSK2 (RPS6KA3).
Purification Protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human RSK3.
Isotype Ig

Target Details RPS6KA3

Product Details anti-RPS6KA3 Antibody Application Details Handling References for anti-RPS6KA3 Antibody (ABIN360345) Images back to top
Antigen
Alternative Name RPS6KA3 / RSK2 (RPS6KA3 Antibody Abstract)
Background RSK3 is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in the gene have been associated with Coffin-Lowry syndrome (CLS).Synonyms: ISPK-1, ISPK1, MAPKAPK-1b, MAPKAPK1, RSK-2, Ribosomal S6 kinase 2, Ribosomal protein S6 kinase alpha-3, S6K-alpha-3, p90RSK3
Gene ID 6197
NCBI Accession NP_004577
UniProt P51812
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RPS6KA3 Antibody Target Details RPS6KA3 Handling References for anti-RPS6KA3 Antibody (ABIN360345) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-RPS6KA3 Antibody Target Details RPS6KA3 Application Details References for anti-RPS6KA3 Antibody (ABIN360345) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-RPS6KA3 Antibody (ABIN360345)

Product Details anti-RPS6KA3 Antibody Target Details RPS6KA3 Application Details Handling Images back to top
Background publications

Yang, Matsuda, Bialek, Jacquot, Masuoka, Schinke, Li, Brancorsini, Sassone-Corsi, Townes, Hanauer, Karsenty: "ATF4 is a substrate of RSK2 and an essential regulator of osteoblast biology; implication for Coffin-Lowry Syndrome." in: Cell, Vol. 117, Issue 3, pp. 387-98, 2004

Zeniou, Gattoni, Hanauer, Stévenin: "Delineation of the mechanisms of aberrant splicing caused by two unusual intronic mutations in the RSK2 gene involved in Coffin-Lowry syndrome." in: Nucleic acids research, Vol. 32, Issue 3, pp. 1214-23, 2004

Guimiot, Delezoide, Hanauer, Simonneau: "Expression of the RSK2 gene during early human development." in: Gene expression patterns : GEP, Vol. 4, Issue 1, pp. 111-4, 2003

Vaidyanathan, Ramos: "RSK2 activity is regulated by its interaction with PEA-15." in: The Journal of biological chemistry, Vol. 278, Issue 34, pp. 32367-72, 2003

Zhang, Cho, Petersen, Bode, Zhu, Dong: "Ataxia telangiectasia mutated proteins, MAPKs, and RSK2 are involved in the phosphorylation of STAT3." in: The Journal of biological chemistry, Vol. 278, Issue 15, pp. 12650-9, 2003

Images

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Supplier Images
 image for anti-RPS6KA3 antibody (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3) (N-Term) (ABIN360345) anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3) (N-Term) antibody
 image for anti-RPS6KA3 antibody (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3) (N-Term) (ABIN360345) anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3) (N-Term) antibody (Image 2)