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|Application / Reactivity||Human|
|Dot Blot (DB)||18 Antibodies|
|Enzyme Immunoassay (EIA)||3 Antibodies|
|Flow Cytometry (FACS)||4 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies|
|Immunofluorescence (IF)||11 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||26 Antibodies|
|Immunohistochemistry (IHC)||20 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||16 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Western Blotting (WB)||80 Antibodies|
|Antigen||Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3) Antibodies|
|Conjugate||This RPS6KA3 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-RPS6KA3 AntibodyTarget Details RPS6KA3 Application Details Handling References for anti-RPS6KA3 Antibody (ABIN360345) Images
|Specificity||This antibody reacts to RSK2 (RPS6KA3).|
|Purification||Protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human RSK3.|
Target Details RPS6KA3Product Details anti-RPS6KA3 Antibody Application Details Handling References for anti-RPS6KA3 Antibody (ABIN360345) Images back to top
|Alternative Name||RPS6KA3 / RSK2 (RPS6KA3 Antibody Abstract)|
|Background||RSK3 is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in the gene have been associated with Coffin-Lowry syndrome (CLS).Synonyms: ISPK-1, ISPK1, MAPKAPK-1b, MAPKAPK1, RSK-2, Ribosomal S6 kinase 2, Ribosomal protein S6 kinase alpha-3, S6K-alpha-3, p90RSK3|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-RPS6KA3 Antibody Target Details RPS6KA3 Handling References for anti-RPS6KA3 Antibody (ABIN360345) Images back to top
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-RPS6KA3 Antibody Target Details RPS6KA3 Application Details References for anti-RPS6KA3 Antibody (ABIN360345) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-RPS6KA3 Antibody (ABIN360345)Product Details anti-RPS6KA3 Antibody Target Details RPS6KA3 Application Details Handling Images back to top
Yang, Matsuda, Bialek et al.: "ATF4 is a substrate of RSK2 and an essential regulator of osteoblast biology; implication for Coffin-Lowry Syndrome." in: Cell, Vol. 117, Issue 3, pp. 387-98, 2004 (PubMed).
Zeniou, Gattoni, Hanauer et al.: "Delineation of the mechanisms of aberrant splicing caused by two unusual intronic mutations in the RSK2 gene involved in Coffin-Lowry syndrome." in: Nucleic acids research, Vol. 32, Issue 3, pp. 1214-23, 2004 (PubMed).
Guimiot, Delezoide, Hanauer et al.: "Expression of the RSK2 gene during early human development." in: Gene expression patterns : GEP, Vol. 4, Issue 1, pp. 111-4, 2003 (PubMed).
Vaidyanathan, Ramos: "RSK2 activity is regulated by its interaction with PEA-15." in: The Journal of biological chemistry, Vol. 278, Issue 34, pp. 32367-72, 2003 (PubMed).
Zhang, Cho, Petersen et al.: "Ataxia telangiectasia mutated proteins, MAPKs, and RSK2 are involved in the phosphorylation of STAT3." in: The Journal of biological chemistry, Vol. 278, Issue 15, pp. 12650-9, 2003 (PubMed).
ImagesProduct Details anti-RPS6KA3 Antibody Target Details RPS6KA3 Application Details Handling References for anti-RPS6KA3 Antibody (ABIN360345) back to top