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anti-Human RPS6KA3 antibody for Flow Cytometry

Recommended RPS6KA3 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3) Antibodies
  • CLS
  • HU-3
  • ISPK-1
  • MAPKAPK1B
  • MPK-9
  • MRX19
  • p90-RSK2
  • pp90RSK2
  • RSK
  • Rsk2
  • RSK2
  • S6K-alpha3
Reactivity
Human
126
53
47
6
6
5
3
3
2
1
1
1
1
1
1
1
1
1
1
Host
Mouse
115
23
Clonality
Monoclonal
Conjugate
This RPS6KA3 antibody is un-conjugated
6
6
6
4
4
4
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
90
42
24
22
21
18
11
7
4
3
3
2
2
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Catalog No. ABIN969386
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3068567 ABIN1846061 ICC FACS IF ELISA WB Mouse IgG1 Log in to see
0.0010666476 ABIN1959892 ICC FACS IF IHC (p) ELISA WB Mouse IgG1 Log in to see 4E10
0.0010666476 ABIN2354578 FACS ELISA WB Mouse IgG1 Log in to see 13B685

Top referenced anti-RPS6KA3 antibody for Flow Cytometry

Similar anti-RPS6KA3 Antibodies

Application / Reactivity Human
Dot Blot (DB) 18 Antibodies
ELISA 43 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 24 Antibodies
Immunohistochemistry (IHC) 20 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 80 Antibodies

General

Antigen Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3) Antibodies
Reactivity Human
(126), (53), (47), (6), (6), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(115), (23)
Clonality (Clone)
Monoclonal   ( )
Conjugate This RPS6KA3 antibody is un-conjugated
(6), (6), (6), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(90), (42), (24), (22), (21), (18), (11), (7), (4), (3), (3), (2), (2)
Pubmed 2 references available
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Product Details anti-RPS6KA3 Antibody

Target Details RPS6KA3 Application Details Handling References for anti-RPS6KA3 Antibody (ABIN969386) Images
Purification Ascites
Immunogen Purified recombinant fragment of human RSK2 expressed in E. coli.
Clone 4-00E-10
Isotype IgG1

Target Details RPS6KA3

Product Details anti-RPS6KA3 Antibody Application Details Handling References for anti-RPS6KA3 Antibody (ABIN969386) Images back to top
Antigen
Alternative Name RSK2 (RPS6KA3 Antibody Abstract)
Background This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS).
Molecular Weight 80 kDa
Gene ID 6197
HGNC 6197
Research Area Signaling
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RPS6KA3 Antibody Target Details RPS6KA3 Handling References for anti-RPS6KA3 Antibody (ABIN969386) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-RPS6KA3 Antibody Target Details RPS6KA3 Application Details References for anti-RPS6KA3 Antibody (ABIN969386) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-RPS6KA3 Antibody (ABIN969386)

Product Details anti-RPS6KA3 Antibody Target Details RPS6KA3 Application Details Handling Images back to top
Product cited in:

Dehan, Bassermann, Guardavaccaro, Vasiliver-Shamis, Cohen, Lowes, Dustin, Huang, Taunton, Pagano: "betaTrCP- and Rsk1/2-mediated degradation of BimEL inhibits apoptosis." in: Molecular cell, Vol. 33, Issue 1, pp. 109-16, 2009

Cho, Yao, Pugliese, Malakhova, Bode, Dong: "A regulatory mechanism for RSK2 NH(2)-terminal kinase activity." in: Cancer research, Vol. 69, Issue 10, pp. 4398-406, 2009

Images

Product Details anti-RPS6KA3 Antibody Target Details RPS6KA3 Application Details Handling References for anti-RPS6KA3 Antibody (ABIN969386) back to top
Supplier Images
ELISA image for anti-RPS6KA3 antibody (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3) (ABIN969386) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-RPS6KA3 antibody (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3) (ABIN969386) Figure 2: Flow cytometric analysis of HepG2 cells using RSK2 mouse mAb (green) and ne...
Immunocytochemistry (ICC) image for anti-RPS6KA3 antibody (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3) (ABIN969386) Figure 3: Immunofluorescence analysis of HepG2 cells using RSK2 mouse mAb (green) . B...
Western Blotting (WB) image for anti-RPS6KA3 antibody (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3) (ABIN969386) Figure 4: Western blot analysis using RSK2 mouse mAb against Hela (1), MCF-7 (2), and...
Western Blotting (WB) image for anti-RPS6KA3 antibody (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3) (ABIN969386) Figure 5: Western blot analysis using RSK2 mAb against human RSK2 (AA: 1-212) recombi...