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anti-Human SIRT1 antibody for Immunofluorescence

Recommended SIRT1 Antibody (supplied by: Log in to see )

Antigen
Sirtuin 1 (SIRT1) Antibodies
  • sirtuin 1
  • AA673258
  • SIR2L1
  • Sir2
  • Sir2a
  • Sir2alpha
  • sir2l1
  • sirtuin
  • SIR2
  • sirtuin 1
  • SIRT1
  • SRT1
  • LOC100349271
  • Sirt1
  • sirt1
Reactivity
Human, Mouse (Murine), Primate, Rat (Rattus)
284
135
69
20
14
11
8
7
7
6
5
4
4
3
2
2
2
1
Host
Mouse
193
78
26
6
3
Clonality
Monoclonal
Conjugate
This SIRT1 antibody is un-conjugated
15
13
10
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
247
128
81
62
59
47
40
33
29
7
5
4
4
2
2
2
1
Supplier
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Catalog No. ABIN4353901
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.813965 ABIN565071 IF IHC (p) ELISA WB Mouse IgG1 kappa AA 456-555, partial Log in to see 7B7 3
11.813965 ABIN1109014 EIA FACS IF IHC (p) WB Mouse IgG1 Log in to see 1F3 2
11.813965 ABIN4353903 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 3
11.813965 ABIN614713 EIA IF IHC (p) WB Mouse IgG1 AA 456-555 Log in to see 7B7
11.813965 ABIN3198318 ChIP IC IF IHC IP WB Rabbit Log in to see Polyclonal
11.813965 ABIN1874784 ChIP IF IHC IP WB Rabbit IgG C-Term Log in to see Polyclonal
11.813965 ABIN4353902 ELISA ICC IF WB Chicken N-Term Log in to see Polyclonal
11.813965 ABIN3032583 IF IHC ELISA WB Rabbit Ig Fraction AA 566-601 Log in to see Polyclonal
11.813965 ABIN2403466 ChIP IF IP IHC WB Rabbit IgG Log in to see Polyclonal
11.813965 ABIN1031567 IF ELISA WB Chicken N-Term Log in to see Polyclonal
11.813965 ABIN5079642 ICC IF Rabbit IgG Log in to see Polyclonal
1 ABIN2668278 ICC IF IP WB Mouse IgG1 AA 581-630 Log in to see 2G1-F7 2
1 ABIN1500927 ELISA FACS ICC IF IHC (p) WB Mouse IgG1 Log in to see 1F3
1 ABIN396774 IF WB Mouse IgG1 kappa AA 456-555 Log in to see 7B7
1 ABIN2471078 IF IHC ELISA WB Mouse IgG1 kappa AA 456-555 Log in to see 7B7
1 ABIN1723581 IF IHC (p) ELISA WB Mouse IgG1 kappa AA 456-555 Log in to see 7B7
1 ABIN574445 IF IHC (p) ELISA WB Mouse IgG1, kappa AA 456-555 Log in to see 7B7
1 ABIN4353899 ICC IF IHC IHC (p) SimWes WB Rabbit AA 400-550, Internal Region Log in to see Polyclonal
1 ABIN1999249 ELISA IF IF/ICC IHC (p) Mouse IgG1 AA 193-747 Log in to see 3H10A10
1 ABIN2476505 IF IHC (p) WB Mouse IgG1 AA 456-555 Log in to see 7B7

Top referenced anti-SIRT1 antibody for Immunofluorescence

Similar anti-SIRT1 Antibodies

Application / Reactivity Rat (Rattus) Primate
Chromatin Immunoprecipitation (ChIP) 3 Antibodies
ELISA 34 Antibodies 17 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 17 Antibodies 17 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 19 Antibodies 16 Antibodies
Immunofluorescence (IF) 25 Antibodies 16 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 27 Antibodies 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies 17 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Western Blotting (WB) 52 Antibodies

General

Antigen Sirtuin 1 (SIRT1) Antibodies
Reactivity Human, Mouse (Murine), Primate, Rat (Rattus)
(284), (135), (69), (20), (14), (11), (8), (7), (7), (6), (5), (4), (4), (3), (2), (2), (2), (1)
Host Mouse
(193), (78), (26), (6), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This SIRT1 antibody is un-conjugated
(15), (13), (10), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(247), (128), (81), (62), (59), (47), (40), (33), (29), (7), (5), (4), (4), (2), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-SIRT1 Antibody

Target Details SIRT1 Application Details Handling References for anti-SIRT1 Antibody (ABIN4353901) Images
Purification Ammonium sulfate precipitation
Immunogen Purified recombinant fragment of human SIRT1 (amino acids: 265-452) expressed in E. coli. [UniProt# Q96EB6]
Clone 1F3
Isotype IgG1

Target Details SIRT1

Product Details anti-SIRT1 Antibody Application Details Handling References for anti-SIRT1 Antibody (ABIN4353901) Images back to top
Antigen
Alternative Name Sirtuin 1/SIRT1 (SIRT1 Antibody Abstract)
Background Gene Symbol: SIRT1
Molecular Weight Theoretical MW: 120 kDa
Gene ID 23411
UniProt Q96EB6
Pathways MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-SIRT1 Antibody Target Details SIRT1 Handling References for anti-SIRT1 Antibody (ABIN4353901) Images back to top
Application Notes Western Blot 1:500-1:2000, Flow Cytometry 1 μg per million cells, ELISA 1:10000, Immunohistochemistry 1:200-1:1000, Immunocytochemistry/Immunofluorescence 1:10-1:1000, Immunohistochemistry-Paraffin 1:200-1:1000This SIRT1 (1F3) antibody is useful for Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details References for anti-SIRT1 Antibody (ABIN4353901) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.03 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-SIRT1 Antibody (ABIN4353901)

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling Images back to top
Product cited in:

Lo Iacono, Visco-Comandini, Valzania, Viscomi, Coviello, Giampà, Roscini, Bisicchia, Siracusano, Troisi, Puglisi-Allegra, Carola: "Adversity in childhood and depression: linked through SIRT1." in: Translational psychiatry, Vol. 5, pp. e629, 2015 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Free Floating)

Images

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling References for anti-SIRT1 Antibody (ABIN4353901) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN4353901) Flow Cytometry: SIRT1 Antibody (1F3) [NBP1-51641] - Flow cytometric analysis of K562 ...
Western Blotting (WB) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN4353901) Western Blot: SIRT1 Antibody (1F3) [NBP1-51641] - Western blot analysis using SIRT1 m...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN4353901) Immunohistochemistry-Paraffin: SIRT1 Antibody (1F3) [NBP1-51641] - Immunohistochemica...
Immunofluorescence (IF) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN4353901) Immunocytochemistry/Immunofluorescence: SIRT1 Antibody (1F3) [NBP1-51641] - Analysis ...
Immunofluorescence (IF) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN4353901) Immunocytochemistry/Immunofluorescence: SIRT1 Antibody (1F3) [NBP1-51641] - SIRT1 ant...
Flow Cytometry (FACS) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN4353901) Flow Cytometry: SIRT1 Antibody (1F3) [NBP1-51641] - Intracellular flow cytometric sta...