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anti-Human SIRT1 antibody for Immunohistochemistry (Frozen Sections)

Recommended SIRT1 Antibody (supplied by: Log in to see )

Antigen
Sirtuin 1 (SIRT1) Antibodies
  • AA673258
  • SIR2
  • Sir2
  • Sir2a
  • Sir2alpha
  • SIR2L1
  • sir2l1
  • sirtuin
  • sirtuin 1
Epitope
C-Term
30
21
20
17
17
16
13
13
10
5
4
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
253
104
46
8
8
6
4
4
3
3
2
2
2
2
1
1
Host
Rabbit
183
57
25
7
2
Clonality
Monoclonal
Conjugate
This SIRT1 antibody is un-conjugated
14
12
9
5
5
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
213
112
57
42
41
34
28
25
11
5
4
4
4
2
1
1
Supplier
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Available images

Catalog No. ABIN258470
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088254584 ABIN3201829 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 244-498 Log in to see
0.00088254584 ABIN1176381 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 244-498 Log in to see Polyclonal

Similar anti-SIRT1 Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 4 Antibodies
ELISA 95 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 11 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 27 Antibodies
Immunofluorescence (IF) 43 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 58 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 40 Antibodies
Immunoprecipitation (IP) 29 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 204 Antibodies

General

Antigen Sirtuin 1 (SIRT1) Antibodies
Epitope C-Term
(30), (21), (20), (17), (17), (16), (13), (13), (10), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(253), (104), (46), (8), (8), (6), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1)
Host Rabbit
(183), (57), (25), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This SIRT1 antibody is un-conjugated
(14), (12), (9), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(213), (112), (57), (42), (41), (34), (28), (25), (11), (5), (4), (4), (4), (2), (1), (1)
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Product Details anti-SIRT1 Antibody

Target Details SIRT1 Application Details Handling Images
Specificity The antibody does not cross-react with other sirtuin family members.
No Cross-Reactivity Rat (Rattus)
Purification IgG purified
Immunogen A synthetic peptide corresponding to residues in C-terminus of human Sir2/SIRT1 was used as immunogen.
Clone E104
Isotype IgG

Target Details SIRT1

Product Details anti-SIRT1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name SIRT1 (SIRT1 Antibody Abstract)
Background The S. cerevisiae silent information regulator 2 (Sir2) protein is an NAD+ -dependenthistone deacetylase (HDACs) that plays a critical role in transcriptional silencing,genome stability, and longevity (1-2). Part of the Sirtuin family (Sir2, Sir3, Sir3), yeastSir2 is the founding member of Class III HDACs. A human homologue of Sir2, SIRT1,regulates the activity of the p53 tumor suppressor and inhibits apoptosis (3-4). Alternate Names: anti-BA57G10.4 antibody, anti-hSIR2 antibody, anti-hSIRT1 antibody, anti-NADdependent deacetylase SIRT1 antibody, anti-NAD dependent deacetylase sirtuin 1antibody, anti-SIR2 like protein 1 antibody, anti-SIR2ALPHA antibody, anti-SIR2alphaprotein antibody, anti-SIR2L1 antibody, anti-SIRT 1 antibody, anti-SIRT1 Sir2 likeproteins (siruitins) type 1 antibody, anti-SIRT1: sirtuin (silent mating type informationregulation 2 homolog) 1 (S. cerevisiae) antibody, anti-sirtuin (silent mating typeinformation regulation 2 homolog) 1 (S. cerevisiae) antibody, anti-sirtuin antibodySirtuin type 1 antibody.
Gene Symbol: SIRT1
Gene ID 23411, 93759, 309757
Research Area Chromatin and Nuclear Signaling, Apoptosis/Necrosis, Proteases
Pathways MAPK Signaling

Application Details

Product Details anti-SIRT1 Antibody Target Details SIRT1 Handling Images back to top
Application Notes In Western blot this antibody detects a band at approximately 110 kDa. Use in IHC-F was reported in the scientific literature (PMID: 21098725).
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:100-250, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Frozen 1:10-1:2000, Immunohistochemistry-Paraffin 1:100-250, Immunoprecipitation 1:50, Western Blot 1:1000-10000
Protocol Protocol specific for SIRT1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-SIRT1 antibody (Sirtuin 1) (C-Term) (ABIN258470) Western blot analysis on Jurkat cell lysate using anti-Sir2 RabMAb (catalog #110-5757...
Immunohistochemistry (IHC) image for anti-SIRT1 antibody (Sirtuin 1) (C-Term) (ABIN258470) Immunohistochemical analysis of paraffin-embedded human colon carcinoma using anti-Si...
Immunohistochemistry (IHC) image for anti-SIRT1 antibody (Sirtuin 1) (C-Term) (ABIN258470) Immunohistochemical analysis of paraffin-embedded human lung squamous carcinoma using...