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anti-Monkey SIRT1 antibody for Flow Cytometry

Recommended SIRT1 Antibody (supplied by: Log in to see )

Antigen
Sirtuin 1 (SIRT1) Antibodies
  • AA673258
  • SIR2
  • Sir2
  • Sir2a
  • Sir2alpha
  • SIR2L1
  • sir2l1
  • sirtuin
  • sirtuin 1
Reactivity
Human, Monkey
216
99
43
7
6
4
2
1
1
1
1
Host
Mouse
151
53
25
6
2
Clonality
Monoclonal
Conjugate
This SIRT1 antibody is un-conjugated
12
11
7
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
176
108
42
34
32
32
25
17
8
5
4
4
2
1
1
Supplier
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Catalog No. ABIN969396
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30754197 ABIN1109014 EIA FACS IF IHC (p) WB Mouse IgG1 Log in to see 1F3 2
0.30754197 ABIN1846081 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see
0.0010660065 ABIN1579514 FACS IF IHC ELISA WB Mouse Log in to see

Top referenced anti-SIRT1 antibody for Flow Cytometry

Similar anti-SIRT1 Antibodies

Application / Reactivity Monkey Human
ELISA 6 Antibodies 92 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 4 Antibodies
Flow Cytometry (FACS) 4 Antibodies 9 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 17 Antibodies
Immunofluorescence (IF) 4 Antibodies 33 Antibodies
Immunohistochemistry (IHC) 4 Antibodies 44 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies 31 Antibodies
Western Blotting (WB) 8 Antibodies
Chromatin Immunoprecipitation (ChIP) 4 Antibodies
ELISA (Capture) 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies

General

Antigen Sirtuin 1 (SIRT1) Antibodies
Reactivity Human, Monkey
(216), (99), (43), (7), (6), (4), (2), (1), (1), (1), (1)
Host Mouse
(151), (53), (25), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This SIRT1 antibody is un-conjugated
(12), (11), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(176), (108), (42), (34), (32), (32), (25), (17), (8), (5), (4), (4), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-SIRT1 Antibody

Target Details SIRT1 Application Details Handling References for anti-SIRT1 Antibody (ABIN969396) Images
Purification Ascites
Immunogen Purified recombinant fragment of human SIRT1 expressed in E. coli.
Clone 1F3
Isotype IgG1

Target Details SIRT1

Product Details anti-SIRT1 Antibody Application Details Handling References for anti-SIRT1 Antibody (ABIN969396) Images back to top
Antigen
Alternative Name SIRT1 (SIRT1 Antibody Abstract)
Background The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53.SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress.SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues, with high expression in heart, brain and skeletal muscle and low expression in lung and placenta. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.
Molecular Weight 120 kDa
Gene ID 23411
HGNC 23411
Research Area Chromatin and Nuclear Signaling, Apoptosis/Necrosis, Proteases
Pathways MAPK Signaling

Application Details

Product Details anti-SIRT1 Antibody Target Details SIRT1 Handling References for anti-SIRT1 Antibody (ABIN969396) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000,
WB: 1/500 - 1/2000,
IHC: 1/200 - 1/1000,
ICC: 1/200 - 1/1000,
FCM: 1/200 - 1/400

Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details References for anti-SIRT1 Antibody (ABIN969396) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-SIRT1 Antibody (ABIN969396)

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling Images back to top
Product cited in:

Xie, Park, Kim, Hwang, Oh, Park, Shong, Lee, Choi: "Transcriptional corepressor SMILE recruits SIRT1 to inhibit nuclear receptor estrogen receptor-related receptor gamma transactivation." in: The Journal of biological chemistry, Vol. 284, Issue 42, pp. 28762-74, 2009

Cha, Noh, Kwon, Kim, Park, Park, Lee, Chung, Kang, Lee, Moon, Jang: "Expression of DBC1 and SIRT1 is associated with poor prognosis of gastric carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 13, pp. 4453-9, 2009

Images

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling References for anti-SIRT1 Antibody (ABIN969396) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN969396) Figure 1: Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and ne...
Immunocytochemistry (ICC) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN969396) Figure 2: Immunofluorescence analysis of NTERA-2 cells using SIRT1 mouse mAb (green) ...
Immunohistochemistry (IHC) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN969396) Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left...
Western Blotting (WB) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN969396) Figure 4: Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), ...