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|Antigen||Sirtuin 1 (SIRT1) Antibodies|
|Epitope||AA 207-256 Alternatives|
|Reactivity||Bat, Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Zebrafish Alternatives|
|Conjugate||This SIRT1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-SIRT1 AntibodyTarget Details SIRT1 Application Details Handling Images
|Predicted Reactivity||Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Xenopus (92%) Chicken (85%) Zebrafish (84%).|
Synthetic peptide located between aa207-256 of human SIRT1 (Q96EB6, NP_036370). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Turkey, Xenopus (92%), Chicken (85%), Zebrafish (84%).
Type of Immunogen: Synthetic peptide
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|Alternative Name||SIRT1 / Sirtuin 1 (SIRT1 Antibody Abstract)|
Name/Gene ID: SIRT1
Synonyms: SIRT1, SIR2-like protein 1, SIR2alpha, SIR2L1, Sirtuin 1, Sir2-like 1, Sirtuin type 1, HSIR2, HSIRT1
|Pathways||MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling|
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Approved: IHC-P (5 μg/mL), WB (0.5 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.
|Restrictions||For Research Use only|
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|Buffer||Lyophilized from PBS with 2 % sucrose|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C,-20 °C|
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
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