anti-Monkey SIRT1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended SIRT1 Antibody (supplied by: Log in to see )

Antigen
Sirtuin 1 (SIRT1) Antibodies
  • sirtuin 1
  • AA673258
  • SIR2L1
  • Sir2
  • Sir2a
  • Sir2alpha
  • sir2l1
  • sirtuin
  • SIR2
  • sirtuin 1
  • SIRT1
  • SRT1
  • LOC100349271
  • Sirt1
  • sirt1
Epitope
AA 207-256
29
24
21
18
16
15
15
14
10
6
6
5
4
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Bat, Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Zebrafish
306
137
69
21
13
10
6
6
6
6
4
3
3
2
2
2
1
1
Host
Rabbit
205
87
27
6
3
Clonality
Polyclonal
Conjugate
This SIRT1 antibody is un-conjugated
17
14
10
7
7
6
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
255
132
88
67
60
51
42
40
36
7
6
5
4
2
2
2
2
2
1
1
Supplier
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Catalog No. ABIN214144
$ 676.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1109014 EIA FACS IF IHC (p) WB Mouse IgG1 Log in to see 1F3 2
1 ABIN1493902 IHC (p) ELISA WB Goat AA 577-590 Log in to see Polyclonal
1 ABIN1500927 ELISA FACS ICC IF IHC (p) WB Mouse IgG1 Log in to see 1F3
1 ABIN1739276 IHC (p) ELISA WB Goat Internal Region Log in to see Polyclonal

General

Antigen Sirtuin 1 (SIRT1) Antibodies
Epitope AA 207-256
(29), (24), (21), (18), (16), (15), (15), (14), (10), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bat, Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Zebrafish
(306), (137), (69), (21), (13), (10), (6), (6), (6), (6), (4), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(205), (87), (27), (6), (3)
Clonality
Polyclonal
Conjugate This SIRT1 antibody is un-conjugated
(17), (14), (10), (7), (7), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(255), (132), (88), (67), (60), (51), (42), (40), (36), (7), (6), (5), (4), (2), (2), (2), (2), (2), (1), (1)
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Product Details anti-SIRT1 Antibody

Target Details SIRT1 Application Details Handling Images
Brand IHC-plus™
Specificity Human SIRT1
Predicted Reactivity Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Xenopus (92%) Chicken (85%) Zebrafish (84%).
Purification Immunoaffinity purified
Immunogen Synthetic peptide located between aa207-256 of human SIRT1 (Q96EB6, NP_036370). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Turkey, Xenopus (92%), Chicken (85%), Zebrafish (84%).

Type of Immunogen: Synthetic peptide
Isotype IgG

Target Details SIRT1

Product Details anti-SIRT1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name SIRT1 / Sirtuin 1 (SIRT1 Antibody Abstract)
Background Name/Gene ID: SIRT1
Family: ADP-Ribosyltransferase

Synonyms: SIRT1, SIR2-like protein 1, SIR2alpha, SIR2L1, Sirtuin 1, Sir2-like 1, Sirtuin type 1, HSIR2, HSIRT1
Gene ID 23411
NCBI Accession NP_036370
UniProt Q96EB6
Pathways MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-SIRT1 Antibody Target Details SIRT1 Handling Images back to top
Application Notes Approved: IHC-P (5 μg/mL), WB (0.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.
Restrictions For Research Use only

Handling

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Images back to top
Format Lyophilized
Reconstitution Distilled water
Concentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.

Images

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling back to top
Supplier Images
 image for anti-Sirtuin 1 (SIRT1) (AA 207-256) antibody (ABIN214144) Liver