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anti-Mouse (Murine) SIRT1 antibody for ELISA

Recommended SIRT1 Antibody (supplied by: Log in to see )

Antigen
Sirtuin 1 (SIRT1) Antibodies
  • AA673258
  • SIR2
  • Sir2
  • Sir2a
  • Sir2alpha
  • SIR2L1
  • sir2l1
  • sirtuin
  • sirtuin 1
Epitope
Internal Region
23
21
20
17
16
13
13
10
10
5
4
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
240
98
43
8
6
4
2
1
1
1
1
Host
Goat
174
54
24
6
2
Clonality
Polyclonal
Conjugate
This SIRT1 antibody is un-conjugated
14
12
9
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
199
108
48
34
32
32
25
18
9
5
4
4
2
1
1
Supplier
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Catalog No. ABIN185702
$ 318.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012145293 ABIN1450758 IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal 3
0.0012145293 ABIN2460448 ELISA WB Rabbit Log in to see Polyclonal
0.0012145293 ABIN2460449 ELISA WB Rabbit Log in to see Polyclonal
0.0012145293 ABIN3182378 ELISA WB Rabbit IgG pSer47 Log in to see Polyclonal
0.0012145293 ABIN1078528 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 236-490 Log in to see Polyclonal
0.0012145293 ABIN3186962 ELISA IHC WB Rabbit IgG Tyr631 Log in to see Polyclonal
0.0012145293 ABIN1457379 IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
0.0012145293 ABIN1031567 IF ELISA WB Chicken N-Term Log in to see Polyclonal
0.0012145293 ABIN2505459 IHC ELISA WB Rabbit IgG Ser47 Log in to see Polyclonal
0.0012145293 ABIN2571305 ELISA Biotin Rabbit IgG AA 236-490 Log in to see Polyclonal
0.0012145293 ABIN2422139 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012145293 ABIN1092423 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012145293 ABIN1092424 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012145293 ABIN2360958 ELISA WB Alkaline Phosphatase (AP) Goat Log in to see Polyclonal
0.0012145293 ABIN2360959 ELISA WB APC Goat Log in to see Polyclonal
0.0012145293 ABIN2360960 ELISA WB Biotin Goat Log in to see Polyclonal
0.0012145293 ABIN2360961 ELISA WB FITC Goat Log in to see Polyclonal
0.0012145293 ABIN2360963 ELISA WB PE Goat Log in to see Polyclonal
0.0012145293 ABIN2505457 ELISA WB Rabbit IgG pSer47 Log in to see Polyclonal
0.0012145293 ABIN2571303 ELISA Rabbit IgG AA 236-490 Log in to see Polyclonal

Top referenced anti-SIRT1 antibody for ELISA

Similar anti-SIRT1 Antibodies

Application / Reactivity Mouse (Murine)
ELISA 38 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 13 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 63 Antibodies

General

Antigen Sirtuin 1 (SIRT1) Antibodies
Epitope Internal Region
(23), (21), (20), (17), (16), (13), (13), (10), (10), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(240), (98), (43), (8), (6), (4), (2), (1), (1), (1), (1)
Host Goat
(174), (54), (24), (6), (2)
Clonality
Polyclonal
Conjugate This SIRT1 antibody is un-conjugated
(14), (12), (9), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application ELISA, Western Blotting (WB)
(199), (108), (48), (34), (32), (32), (25), (18), (9), (5), (4), (4), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-SIRT1 Antibody

Target Details SIRT1 Application Details Handling References for anti-SIRT1 Antibody (ABIN185702) Images
Blocking Peptide Blocking peptide for this product available: ABIN368993
Cross-Reactivity Human, Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen Peptide with sequence C-EITEKPPRTQKE, from the internal region of the protein sequence according to NP_036370.2.

Target Details SIRT1

Product Details anti-SIRT1 Antibody Application Details Handling References for anti-SIRT1 Antibody (ABIN185702) Images back to top
Antigen
Alternative Name SIRT1 (SIRT1 Antibody Abstract)
Background Synonyms: SIRT1, sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae), RP11-57G10.3, SIR2L1, OTTHUMP00000060745, SIR2alpha, sir2-like 1, sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1, sirtuin 1, sirtuin type 1
Gene ID 23411, 93759, 309757
NCBI Accession NP_036370
Research Area Chromatin and Nuclear Signaling, Apoptosis/Necrosis, Proteases
Pathways MAPK Signaling

Application Details

Product Details anti-SIRT1 Antibody Target Details SIRT1 Handling References for anti-SIRT1 Antibody (ABIN185702) Images back to top
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 120 kDa band observed in Mouse fetal Brain lysates (calculated MW of 80.3 kDa according to Muose NP_062786.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Acad Sci U S A. 2003 Sep 16,100(19):10794-9., PMID: 12960381). Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only

Handling

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details References for anti-SIRT1 Antibody (ABIN185702) Images back to top
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-SIRT1 Antibody (ABIN185702)

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling Images back to top
Background publications

Pruitt, Zinn, Ohm, McGarvey, Kang, Watkins, Herman, Baylin: "Inhibition of SIRT1 reactivates silenced cancer genes without loss of promoter DNA hypermethylation." in: PLoS genetics, Vol. 2, Issue 3, pp. e40, 2006

Chen, Steele, Lindquist, Guarente: "Increase in activity during calorie restriction requires Sirt1." in: Science (New York, N.Y.), Vol. 310, Issue 5754, pp. 1641, 2005

Images

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling References for anti-SIRT1 Antibody (ABIN185702) back to top
Supplier Images
 image for anti-SIRT1 antibody (Sirtuin 1) (Internal Region) (ABIN185702) ABIN185702 (0.1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Pri...