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anti-Rat (Rattus) SIRT1 antibody for Western Blotting

Recommended SIRT1 Antibody (supplied by: Log in to see )

Antigen
Sirtuin 1 (SIRT1) Antibodies
  • sirtuin 1
  • AA673258
  • SIR2L1
  • Sir2
  • Sir2a
  • Sir2alpha
  • sir2l1
  • sirtuin
  • SIR2
  • sirtuin 1
  • SIRT1
  • SRT1
  • LOC100349271
  • Sirt1
  • sirt1
Epitope
N-Term
29
23
20
18
16
15
15
14
10
5
5
3
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis
288
139
71
21
14
10
6
6
6
5
5
3
3
2
2
2
2
1
Host
Rabbit
192
81
27
7
3
Clonality
Polyclonal
Conjugate
This SIRT1 antibody is un-conjugated
15
13
10
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
Application
Western Blotting (WB)
251
133
83
62
59
48
40
31
30
5
5
4
4
2
2
2
1
Options
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Catalog No. ABIN2779607
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1003003 ABIN2779608 WB Rabbit N-Term Log in to see Polyclonal 2
3.1003003 ABIN729853 IF (p) IHC (p) WB Rabbit IgG AA 192-242 Log in to see Polyclonal 1
3.1003003 ABIN4353901 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 1F3 1
3.1003003 ABIN3198318 ChIP IC IF IHC IP WB Rabbit Log in to see Polyclonal
3.1003003 ABIN2460449 ELISA WB Rabbit Log in to see Polyclonal
3.1003003 ABIN2460448 ELISA WB Rabbit Log in to see Polyclonal
3.1003003 ABIN1525672 IHC WB Rabbit IgG C-Term Log in to see Polyclonal
3.1003003 ABIN3020731 IHC WB Rabbit IgG C-Term Log in to see Polyclonal
3.1003003 ABIN1874783 IHC IP WB Rabbit IgG Log in to see Polyclonal
3.1003003 ABIN1860560 ICC IHC IP WB Rabbit IgG AA 238-482 Log in to see Polyclonal
3.1003003 ABIN1526120 WB Goat AA 577-590 Log in to see Polyclonal
3.1003003 ABIN4353902 ELISA ICC IF WB Chicken N-Term Log in to see Polyclonal
3.1003003 ABIN2559927 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
3.1003003 ABIN2422139 ELISA WB Rabbit IgG Log in to see Polyclonal
3.1003003 ABIN2403466 ChIP IF IP IHC WB Rabbit IgG Log in to see Polyclonal
3.1003003 ABIN1031567 IF ELISA WB Chicken N-Term Log in to see Polyclonal
3.1003003 ABIN2505456 IF ELISA WB Chicken N-Term Log in to see Polyclonal
3.1003003 ABIN2502803 WB Goat AA 577-590 Log in to see Polyclonal
1 ABIN783610 EIA WB Chicken N-Term Log in to see Polyclonal 1
1 ABIN493460 WB Rabbit IgG AA 33-55 Log in to see Polyclonal 4

Top referenced anti-SIRT1 antibody for Western Blotting

  • Chicken Polyclonal SIRT1 Primary Antibody for WB - ABIN2779607 (4 Publications): Solomon, Pasupuleti, Xu, McDonagh, Curtis, DiStefano, Huber: Inhibition of SIRT1 catalytic activity increases p53 acetylation but does not alter cell survival following DNA damage. in Molecular and cellular biology 2005 (PubMed)

  • Dog (Canine) Polyclonal SIRT1 Primary Antibody for WB - ABIN2779608 (2 Publications): Anavekar, Mirza, Skali, Plappert, St John Sutton, Pfeffer, Solomon,: Risk assessment in patients with depressed left ventricular function after myocardial infarction using the myocardial performance index--Survival and Ventricular Enlargement (SAVE) experience. in Journal of the American Society of Echocardiography : official publication of the American Society of Echocardiography 2006 (PubMed)

  • Human Polyclonal SIRT1 Primary Antibody for IF (p), IHC (p) - ABIN729853 (1 Publications): Mohan, Kumar, Lackner, Alvarez: Dysregulated miR-34a-SIRT1-acetyl p65 axis is a potential mediator of immune activation in the colon during chronic simian immunodeficiency virus infection of rhesus macaques. in Journal of immunology (Baltimore, Md. : 1950) 2014 (PubMed)

  • Human Monoclonal SIRT1 Primary Antibody for ELISA, FACS - ABIN4353901 (1 Publications): Lo Iacono, Visco-Comandini, Valzania, Viscomi, Coviello, Giampà, Roscini, Bisicchia, Siracusano, Troisi, Puglisi-Allegra, Carola: Adversity in childhood and depression: linked through SIRT1. in Translational psychiatry 2015 (PubMed)

Similar anti-SIRT1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken Dog (Canine) Pig (Porcine) Xenopus laevis
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies 1 Antibodies 1 Antibodies
Simple Western (SimWes) 4 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 232 Antibodies 6 Antibodies 10 Antibodies 95 Antibodies 6 Antibodies 54 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 31 Antibodies 3 Antibodies 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 58 Antibodies

General

Antigen Sirtuin 1 (SIRT1) Antibodies
Epitope N-Term
(29), (23), (20), (18), (16), (15), (15), (14), (10), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis
(288), (139), (71), (21), (14), (10), (6), (6), (6), (5), (5), (3), (3), (2), (2), (2), (2), (1)
Host Rabbit
(192), (81), (27), (7), (3)
Clonality
Polyclonal
Conjugate This SIRT1 antibody is un-conjugated
(15), (13), (10), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Application Western Blotting (WB)
(251), (133), (83), (62), (59), (48), (40), (31), (30), (5), (5), (4), (4), (2), (2), (2), (1)
Pubmed 4 references available
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Product Details anti-SIRT1 Antibody

Target Details SIRT1 Application Details Handling References for anti-SIRT1 Antibody (ABIN2779607) Images
Blocking Peptide Blocking peptide for this product available: ABIN983762
Sequence PETIPPPELD DMTLWQIVIN ILSEPPKRKK RKDINTIEDA VKLLQECKKI
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, African clawed frog : 90 %, Chicken : 80 %, Zebrafish : 80 %
Characteristics This is a rabbit polyclonal antibody against SIRT1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human SIRT1

Target Details SIRT1

Product Details anti-SIRT1 Antibody Application Details Handling References for anti-SIRT1 Antibody (ABIN2779607) Images back to top
Antigen
Alternative Name SIRT1 (SIRT1 Antibody Abstract)
Background SIRT1 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined,
Molecular Weight 82 kDa
Gene ID 23411
NCBI Accession NM_012238, NP_036370
UniProt Q96EB6
Research Area Chromatin and Nuclear Signaling, Apoptosis/Necrosis, Proteases
Pathways MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis

Application Details

Product Details anti-SIRT1 Antibody Target Details SIRT1 Handling References for anti-SIRT1 Antibody (ABIN2779607) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 747 AA

Restrictions For Research Use only

Handling

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details References for anti-SIRT1 Antibody (ABIN2779607) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-SIRT1 Antibody (ABIN2779607)

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling Images back to top
Product cited in:

Cueno, Imai, Tamura, Ochiai: "Butyric acid-induced rat jugular blood cytosolic oxidative stress is associated with SIRT1 decrease." in: Cell stress & chaperones, Vol. 19, Issue 2, pp. 295-8, 2014

Hsu, Chuang: "Changes in energy-regulated molecules in the trophocytes and fat cells of young and old worker honeybees (Apis mellifera)." in: The journals of gerontology. Series A, Biological sciences and medical sciences, Vol. 69, Issue 8, pp. 955-64, 2014

Anavekar, Mirza, Skali, Plappert, St John Sutton, Pfeffer, Solomon,: "Risk assessment in patients with depressed left ventricular function after myocardial infarction using the myocardial performance index--Survival and Ventricular Enlargement (SAVE) experience." in: Journal of the American Society of Echocardiography : official publication of the American Society of Echocardiography, Vol. 19, Issue 1, pp. 28-33, 2006

Background publications

Solomon, Pasupuleti, Xu, McDonagh, Curtis, DiStefano, Huber: "Inhibition of SIRT1 catalytic activity increases p53 acetylation but does not alter cell survival following DNA damage." in: Molecular and cellular biology, Vol. 26, Issue 1, pp. 28-38, 2005

Images

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling References for anti-SIRT1 Antibody (ABIN2779607) back to top
Supplier Images
Western Blotting (WB) image for anti-SIRT1 antibody (Sirtuin 1) (N-Term) (ABIN2779607) anti-Sirtuin 1 (SIRT1) (N-Term) antibody