anti-Human YAP1 antibody for Dot Blot

Recommended YAP1 Antibody (supplied by: Log in to see )

Antigen
Yes-Associated Protein 1 (YAP1) Antibodies
  • YAP1
  • YAP
  • YAP2
  • YAP65
  • YKI
  • AI325207
  • Yap
  • Yap65
  • Yki
  • Yorkie
  • cb194
  • sb:cb194
  • si:ch211-181p1.5
  • si:dkey-3b8.3
  • wu:fc18c04
  • zgc:158380
  • xyap
  • yap
  • yap2
  • yap65
  • yki
  • HCCA1
  • HCCA2
  • YY1AP
  • Yes-associated protein 1
  • Yes-associated protein 1, 65kDa
  • yes-associated protein 1
  • YY1 associated protein 1
  • YAP1
  • LOC787360
  • LOC100219556
  • Yap1
  • yap1
  • YY1AP1
Epitope
pSer127
21
21
19
15
15
14
14
12
9
9
7
7
7
6
4
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
244
93
80
9
6
6
4
3
3
3
3
3
2
1
1
Host
Rabbit
164
65
16
1
Clonality
Polyclonal
Conjugate
This YAP1 antibody is un-conjugated
9
9
9
7
6
6
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB), ELISA
190
110
67
55
42
38
31
26
15
13
6
3
3
3
1
1
Supplier
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Catalog No. ABIN3029660
$ 411.13
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2391012 DB ELISA Rabbit IgG AA 119-133, Ser127 Log in to see Polyclonal
1 ABIN2391002 DB ELISA APC Rabbit IgG Ser127, AA 119-133 Log in to see Polyclonal
1 ABIN2391004 DB ELISA FITC Rabbit IgG Ser127, AA 119-133 Log in to see Polyclonal
1 ABIN2391005 DB ELISA HRP Rabbit IgG Ser127, AA 119-133 Log in to see Polyclonal
1 ABIN2391006 DB ELISA PE Rabbit IgG Ser127, AA 119-133 Log in to see Polyclonal
1 ABIN2391001 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG Ser127, AA 119-133 Log in to see Polyclonal
1 ABIN2391003 DB ELISA Biotin Rabbit IgG Ser127, AA 119-133 Log in to see Polyclonal
1 ABIN1952746 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG Ser127, AA 119-133 Log in to see Polyclonal
1 ABIN1958391 DB ELISA PE Rabbit IgG Ser127, AA 119-133 Log in to see Polyclonal
1 ABIN1953618 DB ELISA APC Rabbit IgG Ser127, AA 119-133 Log in to see Polyclonal
1 ABIN1956065 DB ELISA FITC Rabbit IgG Ser127, AA 119-133 Log in to see Polyclonal
1 ABIN2568987 DB ELISA Rabbit IgG AA 119-133, pSer127 Log in to see Polyclonal
1 ABIN4955226 DB Rabbit Ig Fraction pSer127 Log in to see Polyclonal
1 ABIN1811022 DB Rabbit pSer127 Log in to see Polyclonal
1 ABIN2794342 DB Rabbit Ig Fraction pSer127 Log in to see Polyclonal

General

Antigen Yes-Associated Protein 1 (YAP1) Antibodies
Epitope pSer127
(21), (21), (19), (15), (15), (14), (14), (12), (9), (9), (7), (7), (7), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(244), (93), (80), (9), (6), (6), (4), (3), (3), (3), (3), (3), (2), (1), (1)
Host Rabbit
(164), (65), (16), (1)
Clonality
Polyclonal
Conjugate This YAP1 antibody is un-conjugated
(9), (9), (9), (7), (6), (6), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Dot Blot (DB), ELISA
(190), (110), (67), (55), (42), (38), (31), (26), (15), (13), (6), (3), (3), (3), (1), (1)
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Product Details anti-YAP1 Antibody

Target Details YAP1 Application Details Handling Images
Cross-Reactivity (Details) Expected species reactivity: Mouse,Rat,Chicken
Purification Antigen affinity purified
Immunogen This phospho-YAP antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS127 of human YAP1.
Isotype Ig Fraction

Target Details YAP1

Product Details anti-YAP1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name YAP (YAP1 Antibody Abstract)
Background Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).
UniProt P46937
Pathways MAPK Signaling, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha

Application Details

Product Details anti-YAP1 Antibody Target Details YAP1 Handling Images back to top
Application Notes Titration of the phospho-YAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500
Restrictions For Research Use only

Handling

Product Details anti-YAP1 Antibody Target Details YAP1 Application Details Images back to top
Format Liquid
Buffer In 1X PBS, pH 7.4, with 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Aliquot the phospho-YAP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

Product Details anti-YAP1 Antibody Target Details YAP1 Application Details Handling back to top
Supplier Images
Dot Blot (DB) image for anti-Yes-Associated Protein 1 (YAP1) (pSer127) antibody (ABIN3029660) Dot blot analysis of phospho-YAP antibody. 50ng of phos-peptide or nonphos-peptide pe...