Prestained Dual Color Protein Molecular Weight Marker

Details for Product No. ABIN1113047, Supplier: Log in to see
Detection Range
10-250 kDa
Minimum Detection Limit
10 kDa
Application
SDS-PAGE (SDS), Western Blotting (WB)
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Purpose Prestained Protein Molecular Weight Marker is a three-color molecular weight standard, contains 9 prestained natural proteins with molecular weights from 10 kDa to 250 kDa. It has two orange reference bands-70 kDa band and 25 kDa band.
Detection Method Colorimetric
Characteristics Prestained protein ladders / marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verifying of Western transfer efficiency on PVDF, nylon and nitrocellulose membranes and approximate sizing of proteins. It is composed of prestained recombinant prokaryotic proteins.
Plate Strips (12 x 8)
Assay Procedure
  1. Thaw the ladder either at room temperature or at 37°C for a few minutes to dissolve precipitated solids thoroughly. Do not boil! 2. Mix gently, but thoroughly, to ensure that the solution is homogeneous. 3. Load the following volumes of the ladder on Min SDS-polyacrylamide gel: 5 myl per well, with a thickness of 0.75-1.0 mm Large SDS-polyacrylamide gel: 10 myl per well, with a thickness of 0.75-1.0 mm
Restrictions For Research Use only
Format Liquid
Buffer 62.5 mM Tris-H3PO4 (pH 7.5, 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol
Preservative Sodium azide, Thimerosal (Merthiolate)
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C for 3 months, and at -20 °C for long term.