20bp DNA Ladder

Details for Product No. ABIN1540464, Supplier: Log in to see
Detection Range
60-300 bp
Minimum Detection Limit
60 bp
Application
Agarose Gel Electrophoresis (AGE)
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Purpose 20bp Ladder is ideal for determining the size of double-stranded DNA from 60 to 300 base pairs.
Detection Method Fluorometric
Characteristics The ladder consists of 13 linear double-stranded fragments. The 100bp and 200bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µL loading, all fragments except 100bp and 200bp are 40ng. The 100bp and 200bp fragment is 100ng. This ladder is pre-mixed with loading dye and is ready to use.
13 DNA Fragments (bp): 60, 80, 100, 120, 140, 160, 180, 200, 220, 240, 260, 280, and 300
100bp and 200bp Bands: 100ng/5 µL
Other Bands: 40ng/5 µL
Range: 60bp-300bp
Bands: 13
Application Notes Recommended Loading: 5 µL/Lane.
Add 5 µL ladder directly to each well. You may need more or less than 5 µL of ladder, depending on well size and level of intensity needed to visualize the bands.
Recommended Loading: 5 µL/Lane, 8cm, 4% Agarose Gel, 1×TBE, 5V/cm, 1h.
Comment

Do not heat before loading.
For quantification, adjust the concentration of the sample to equalize.
Visualize DNA by staining with ethidium bromide or with SYBR Green I.

Restrictions For Research Use only
Format Liquid
Storage -20 °C
Storage Comment 1 year at room temperature, for long term storage, please keep at -20°C.
Expiry Date 12 months
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Agarose Gel Electrophoresis (AGE) image for 20bp DNA Ladder (ABIN1540464) 20bp DNA Ladder