50bp DNA Ladder Plus

Details for Product No. ABIN1540466, Supplier: Log in to see
Detection Range
50-1000 bp
Minimum Detection Limit
50 bp
Application
Agarose Gel Electrophoresis (AGE)
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Purpose 50bp DNA Ladder Plus (Quantitative) is ideal for determining the size of double-stranded DNA from 50 to 1000 base pairs.
Detection Method Fluorometric
Characteristics The ladder consists of 13 linear double-stranded DNA fragments, chromatography-purified. The intensity of the 250bp and 500bp has been increased to serve as a reference for easy identification. All fragments are precisely quantified and mixed during the production. For 5 µL loading, all fragments except 250bp and 500bp are at 40ng. The 250bp and 500bp fragments are at 100ng. This ladder is pre-mixed with loading dye and is ready to use.
13 DNA Fragments (bp): 50, 100, 150, 200, 250, 300, 400, 500, 600, 700, 800, 900, and 1000
250bp and 500bp Bands: 100ng/5 µL
Other Bands: 40ng/5 µL
Range: 50bp-1kb
Bands: 13
Application Notes Recommended Loading: 5 µL/Lane.
Add 5 µL ladder directly to each well. You may need more or less than 5 µL of ladder, depending on well size and level of intensity needed to visualize the bands.
Recommended Loading: 5 µL/Lane, 8 cm 3 % Agarose Gel, 0.5×TBE, 5V/cm, 1h.
Comment

Do not heat before loading.
For quantification, adjust the concentration of the sample to equalize.
Visualize DNA by staining with ethidium bromide or with SYBR Green I.

Restrictions For Research Use only
Format Liquid
Storage -20 °C
Storage Comment 1 year at room temperature, for long term storage, please keep at -20°C.
Expiry Date 12 months
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Agarose Gel Electrophoresis (AGE) image for 50bp DNA Ladder Plus (ABIN1540466) 50bp DNA Ladder Plus