Lambda DNA/Hind III Marker

Details for Product No. ABIN1540476, Supplier: Log in to see
Detection Range
564-23130 bp
Minimum Detection Limit
564 bp
Application
Agarose Gel Electrophoresis (AGE)
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Purpose Lambda DNA/ Hind III Marker is valuable for various DNA sizing purposes.
Detection Method Fluorometric
Characteristics Lambda DNA/Hind III Marker was digested with restriction enzyme Hind III, followed by phenol/chloroform extraction to inactivate the enzyme and dialyzed in TE buffer. The final DNA concentration is measured at OD260 and compared to other DNA standards for accuracy. This ladder is pre-mixed with loading dye and is ready to use.
7 DNA Fragments (bp): 564, 2027, 2322, 4361, 6557, 9416, and 23130

Range: 564bp-23130bp
Bands: 7
Application Notes Recommended Loading: 6µL/Lane.
Add 6µL ladder directly to each well. You may need more or less than 6µL of ladder, depending on well size and level of intensity needed to visualize the bands.
Recommended Loading: 6µL/Lane, 8cm, 0.8% Agarose Gel, 0.5xTAE, 7V/cm, 45min.
Comment

Do not heat before loading.
Visualize DNA by staining with ethidium bromide or with SYBR Green I.

Restrictions For Research Use only
Format Liquid
Storage -20 °C
Storage Comment 3 months at room temperature, for long term storage, please keep at -20°C.
Expiry Date 3 months
Supplier Images
Agarose Gel Electrophoresis (AGE) image for Lambda DNA/Hind III Marker (ABIN1540476) Lambda DNA/Hind III Marker