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BLUelf Prestained Protein Ladder

5 kDa - 245 kDa Colorimetric
Catalog No. ABIN2868250
  • Detection Method
    Colorimetric
    Detection Range
    5 kDa - 245 kDa
    Minimum Detection Limit
    5 kDa
    Application
    SDS-PAGE (SDS), Western Blotting (WB)
  • Application Notes
    Use 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
    Comment

    The BLUelf Prestained Protein Ladder is a combination of 13 pre-stained proteins with molecular weights from 5 to 245 kDa. The 13 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa serve as reference bands. The BLUelf Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

    Reagent Preparation

    Quality Control:
    Under suggested conditions, BLUelf Prestained Protein Ladder resolves 13 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to nitrocellulose membrane.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Approximately 0.1~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25°C), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.
    Storage
    4 °C/-20 °C
    Storage Comment
    Stable for up to 2 weeks at 25°C.
    Stable for up to 3 months at 4°C.
    Stable for up to 24 months at -20°C.
    Expiry Date
    24 months
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