BLUeye Prestained Protein Ladder

Details for Product No. ABIN2868252, Supplier: Log in to see
Detection Range
10-245 kDa
Minimum Detection Limit
10 kDa
Application
SDS-PAGE (SDS), Western Blotting (WB)
Options
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Detection Method Colorimetric
Application Notes Use 3 μL or 5 μL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 1.5-2.5 μL per well for general Western transfering.
BLUeye Prestained Protein Ladder resolves 12 major bands in 15 % SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane.
Comment

The BLUeye Prestained Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BLUeye Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.

Reagent Preparation

Quality Control:
Under suggested conditions, BLUeye Prestained Protein Ladder resolves 12 major bands in 15 % SDS-PAGE (Trisglycine buffer) and after Western blotting to nitrocellulose membrane.

Restrictions For Research Use only
Format Liquid
Buffer Approximately 0.1~0.4 mg/mL of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25 °C), 2 % SDS, 1 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol). Bis-Tris 10 % Tris-Glycine Bis-Tris 10 % MES buffer 4~20 % MOPS buffer
Storage RT
Storage Comment Store at 25°C for 6 months, Store at -20°C for 24 months
Expiry Date 24 months
Supplier Images
 image for BLUeye Prestained Protein Ladder (ABIN2868252) BLUeye Prestained Protein Ladder