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BlueRAY Prestained Protein Ladder

10-180 kDa Colorimetric
Catalog No. ABIN2868254
  • Detection Method
    Colorimetric
    Detection Range
    10-180 kDa
    Minimum Detection Limit
    10 kDa
    Application
    SDS-PAGE (SDS), Western Blotting (WB)
  • Application Notes
    Use 3 μL or 5 μL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2-3 μL per well for general Western transfering. Apply more for thicker (>1.5mm) or larger gel.
    5 μL of BlueRAY Prestained Protein Ladder resolves 10 bands in 4-20 % SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.
    Comment

    The BlueRAY Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BlueRAY Prestained Protein Ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use.Do not heat, dilute, add reducing agent before loading.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Approximately 0.1~0.4 mg/mL of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25 °C), 2 % SDS, 1 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol). Tris-Glycine Bis-Tris 12 % Bis-Tris 10 % 4~20 % MOPS buffer MES buffer
    Storage
    RT
    Storage Comment
    Store at 25°C for 6 months, Store at -20°C for 24 months
    Expiry Date
    24 months
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