PiNK Plus Prestained Protein Ladder

Details for Product No. ABIN2868256, Supplier: Log in to see
Detection Range
10-175 kDa
Minimum Detection Limit
10 kDa
Application
SDS-PAGE (SDS), Western Blotting (WB)
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Purpose The PiNK Plus Prestained Protein Ladder contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kDa.
Detection Method Colorimetric
Characteristics Broad range: 10-175 kDa. Easy to identify: includes the -10, -40 and 90 kDa reference bands coupled with an blue dye.
Application Notes Can be used for monitoring of protein migration during SDS-polyacrylamide gel electrophoresis and monitoring of protein transfer onto membranes during Western blotting.
Tested in SDS-polyacrylamide gel electrophoresis and Western blotting.
Comment

The PiNK Plus Prestained Protein Ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency.

Assay Procedure

Recommendations for Loading
1.Thaw the ladder either at room temperature or at 37-40 °C for a few minutes to dissolve precipitated solids. Do not boil.
2.Mix thoroughly to ensure the solution is homogeneous.
3.Load the following volumes of the ladder on SDS-polyacrylamide gel:
- 5 μL per well for mini-gels, 2.5 μL per well for blots
- 10 μL per well for large gels, 5 μL per well for blots

Restrictions For Research Use only
Format Liquid
Buffer Approximately 0.2~0.4 mg/mL of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25 °C), 2 % SDS, 1 mM 2-Mercaptoethanol, 3.6 M Urea, and 15 % (v/v) glycerol).
Storage RT
Storage Comment Store at 25°C for 6 months, Store at -20°C for 24 months
Expiry Date 24 months
Supplier Images
 image for PiNK Plus Prestained Protein Ladder (ABIN2868256) PiNK Plus Prestained Protein Ladder