anti-Human BHMT antibody for Western Blotting

Recommended BHMT Antibody (supplied by: Log in to see )

Antigen
Betaine--Homocysteine S-Methyltransferase (BHMT) Antibodies
  • BHMT1
  • hm:zehn2153
  • wu:fb53h01
  • wu:fb63c08
  • wu:fj64d01
  • zgc:123027
  • betaine--homocysteine S-methyltransferase
  • betaine-homocysteine methyltransferase
  • betaine-homocysteine S-methyltransferase
  • BHMT
  • bhmt
  • Bhmt
Epitope
N-Term
28
18
3
3
3
2
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
111
24
23
18
11
4
3
3
3
3
2
2
2
2
1
1
Host
Rabbit
78
36
20
Clonality
Polyclonal
Conjugate
This BHMT antibody is un-conjugated
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
124
65
52
25
25
14
8
3
1
1
1
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Catalog No. ABIN2773785
$ 229.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9060245 ABIN314263 ELISA IHC WB Goat C-Term Log in to see Polyclonal 4
2.9060245 ABIN968978 IHC ELISA WB Mouse IgG1 Log in to see 8C11H5 2
2.9060245 ABIN614951 EIA IHC (p) WB Goat C-Term Log in to see Polyclonal
2.9060245 ABIN306415 ELISA WB Mouse TBD, kappa chain Log in to see 3D6 1
2.9060245 ABIN2855528 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
2.9060245 ABIN316049 ELISA WB Mouse IgG2a AA 1-406 Log in to see 3D6
2.9060245 ABIN2855542 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
2.9060245 ABIN2466485 IHC ELISA WB Goat C-Term Log in to see Polyclonal
2.9060245 ABIN2462418 IHC ELISA WB Rabbit Log in to see Polyclonal
2.9060245 ABIN2462417 IHC ELISA WB Rabbit Log in to see Polyclonal
2.9060245 ABIN2452808 FACS IF WB Mouse IgG2b Log in to see 10B3
2.9060245 ABIN1871286 IHC WB Rabbit IgG Log in to see Polyclonal
2.9060245 ABIN4284583 FACS ICC IF IHC IHC (p) WB Mouse IgG2a Log in to see 3E11
2.9060245 ABIN442411 ICC IF IHC (p) IHC WB Rabbit IgG Center Log in to see Polyclonal
2.9060245 ABIN3021750 IHC WB Rabbit IgG Log in to see Polyclonal
2.9060245 ABIN4284580 IHC IHC (p) WB Rabbit N-Term Log in to see Polyclonal
2.9060245 ABIN4284582 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
2.9060245 ABIN4284579 ELISA IHC (p) WB Mouse IgG1 Log in to see 8C11H5
2.9060245 ABIN629748 IHC WB Rabbit C-Term Log in to see Polyclonal
2.9060245 ABIN629793 IHC WB Rabbit N-Term Log in to see Polyclonal

General

Antigen Betaine--Homocysteine S-Methyltransferase (BHMT) Antibodies
Epitope N-Term
(28), (18), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
(111), (24), (23), (18), (11), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Host Rabbit
(78), (36), (20)
Clonality
Polyclonal
Conjugate This BHMT antibody is un-conjugated
(4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(124), (65), (52), (25), (25), (14), (8), (3), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-BHMT Antibody

Target Details BHMT Application Details Handling ProductDetails: References for anti-BHMT antibody (ABIN2773785) Images
Sequence AVEHPEAVRQ LHREFLRAGS NVMQTFTFYA SEDKLENRGN YVLEKISGQE
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%
Characteristics This is a rabbit polyclonal antibody against BHMT. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human BHMT

Target Details BHMT

Product Details anti-BHMT Antibody Application Details Handling ProductDetails: References for anti-BHMT antibody (ABIN2773785) Images back to top
Antigen
Alternative Name BHMT (BHMT Antibody Abstract)
Background BHMT is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in its gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.Betaine-homocysteine methyltransferase is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in BHMT could lead to hyperhomocyst(e)inemia,but such a defect has not yet been observed.
Alias Symbols: BHMT1
Protein Interaction Partner: BHMT, BBS4, BBS2, BBS1, ALG9, LAMTOR3,
Protein Size: 406
Molecular Weight 45 kDa
Gene ID 635
NCBI Accession NM_001713, NP_001704
UniProt Q93088
Research Area Signaling, Metabolism, Amino Acids, Enzymes
Pathways Methionine Biosynthetic Process

Application Details

Product Details anti-BHMT Antibody Target Details BHMT Handling ProductDetails: References for anti-BHMT antibody (ABIN2773785) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 406 AA

Restrictions For Research Use only

Handling

Product Details anti-BHMT Antibody Target Details BHMT Application Details ProductDetails: References for anti-BHMT antibody (ABIN2773785) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-BHMT antibody (ABIN2773785)

Product Details anti-BHMT Antibody Target Details BHMT Application Details Handling Images back to top
Product cited in:

Jiang, Hurt, Breen, Stabler, Allen, Orlicky, Maclean: "Sex-specific dysregulation of cysteine oxidation and the methionine and folate cycles in female cystathionine gamma-lyase null mice: a serendipitous model of the methylfolate trap." in: Biology open, Vol. 4, Issue 9, pp. 1154-62, 2015

Hu, Lausted, Yoo, Yan, Brightman, Chen, Wang, Bu, Hood: "Quantitative liver-specific protein fingerprint in blood: a signature for hepatotoxicity." in: Theranostics, Vol. 4, Issue 2, pp. 215-28, 2014

Maclean, Jiang, Greiner, Allen, Stabler et al.: "Long-term betaine therapy in a murine model of cystathionine beta-synthase deficient homocystinuria: decreased efficacy over time reveals a significant threshold effect between elevated homocysteine ..." in: Molecular genetics and metabolism, Vol. 105, Issue 3, pp. 395-403, 2012

Background publications

Castro, Gratson, Evans, Jiracek, Collinsová, Ludwig, Garrow: "Dissecting the catalytic mechanism of betaine-homocysteine S-methyltransferase by use of intrinsic tryptophan fluorescence and site-directed mutagenesis." in: Biochemistry, Vol. 43, Issue 18, pp. 5341-51, 2004

Images

Product Details anti-BHMT Antibody Target Details BHMT Application Details Handling ProductDetails: References for anti-BHMT antibody (ABIN2773785) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Betaine--Homocysteine S-Methyltransferase (BHMT) (N-Term) antibody (ABIN2773785) anti-Betaine--Homocysteine S-Methyltransferase (BHMT) (N-Term) antibody
Western Blotting (WB) image for anti-Betaine--Homocysteine S-Methyltransferase (BHMT) (N-Term) antibody (ABIN2773785) Host: Rabbit
Target Name: BHMT
Sample Tissue: 293T
Antibody Dilu...