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|Antigen||Cytoplasmic Linker Associated Protein 2 (CLASP2) Antibodies|
|Conjugate||This CLASP2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-CLASP2 AntibodyTarget Details CLASP2 Application Details Handling References for anti-CLASP2 antibody (ABIN359357) Images
|Purification||Protein A Chromatography followed by peptide Affinity Purification.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CLASP2.|
Target Details CLASP2Product Details anti-CLASP2 Antibody Application Details Handling References for anti-CLASP2 antibody (ABIN359357) Images back to top
|Alternative Name||CLASP2 (CLASP2 Antibody Abstract)|
|Background||Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.Synonyms: CLIP-associating protein 2, Cytoplasmic linker-associated protein 2, KIAA0627, Protein Orbit homolog 2, hOrbit2|
|Pathways||Microtubule Dynamics, Maintenance of Protein Location|
Application DetailsProduct Details anti-CLASP2 Antibody Target Details CLASP2 Handling References for anti-CLASP2 antibody (ABIN359357) Images back to top
ELISA: 1/1,000Western blotting: 1/100-1/500Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CLASP2 Antibody Target Details CLASP2 Application Details References for anti-CLASP2 antibody (ABIN359357) Images back to top
|Buffer||PBS containing 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-CLASP2 antibody (ABIN359357)Product Details anti-CLASP2 Antibody Target Details CLASP2 Application Details Handling Images back to top
Pereira, Pereira, Maia, Drabek, Sayas, Hergert, Lince-Faria, Matos, Duque, Stepanova, Rieder, Earnshaw, Galjart, Maiato: "Mammalian CLASP1 and CLASP2 cooperate to ensure mitotic fidelity by regulating spindle and kinetochore function." in: Molecular biology of the cell, Vol. 17, Issue 10, pp. 4526-42, 2006
Mimori-Kiyosue, Grigoriev, Sasaki, Matsui, Akhmanova, Tsukita, Vorobjev: "Mammalian CLASPs are required for mitotic spindle organization and kinetochore alignment." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 11, Issue 8, pp. 845-57, 2006
Lansbergen, Grigoriev, Mimori-Kiyosue, Ohtsuka, Higa, Kitajima, Demmers, Galjart, Houtsmuller, Grosveld, Akhmanova: "CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta." in: Developmental cell, Vol. 11, Issue 1, pp. 21-32, 2006
ImagesProduct Details anti-CLASP2 Antibody Target Details CLASP2 Application Details Handling References for anti-CLASP2 antibody (ABIN359357) back to top