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anti-Human CLASP2 antibody for Western Blotting

Recommended CLASP2 Antibody (supplied by: Log in to see )

Antigen
Cytoplasmic Linker Associated Protein 2 (CLASP2) Antibodies
  • 1500004F14Rik
  • 8030404L10Rik
  • C77448
  • CLASP2beta
  • DKFZp459M112
  • mKIAA0627
  • zgc:77279
Epitope
C-Term
24
23
8
2
2
1
1
1
1
1
1
Reactivity
Human
83
29
20
Host
Rabbit
81
8
2
Clonality
Polyclonal
Conjugate
This CLASP2 antibody is un-conjugated
7
7
7
6
6
6
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
61
49
28
11
6
6
4
2
2
1
Supplier
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Catalog No. ABIN359357
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.1347588 ABIN2789937 WB Rabbit C-Term Log in to see Polyclonal
0.1347588 ABIN652978 FACS WB Rabbit Ig AA 998-1026, pTyr1019 Log in to see Polyclonal
0.1347588 ABIN951541 EIA FACS WB Rabbit Ig AA 1005-1034 Log in to see Polyclonal
0.1347588 ABIN391268 IHC (p) WB Rabbit Ig AA 946-975, C-Term Log in to see Polyclonal
0.1347588 ABIN2786099 WB Rabbit C-Term Log in to see Polyclonal
0.1347588 ABIN439355 ICC IF IP WB Rabbit IgG Log in to see Polyclonal
0.1347588 ABIN2160138 FACS ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.1347588 ABIN2158251 IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.0011759355 ABIN1975410 IHC ELISA WB PE Rabbit IgG C-Term Log in to see Polyclonal
0.0011759355 ABIN1970829 IHC ELISA WB FITC Rabbit IgG C-Term Log in to see Polyclonal
0.0011759355 ABIN1966991 IHC ELISA WB Biotin Rabbit IgG C-Term Log in to see Polyclonal
0.0011759355 ABIN1953302 ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Log in to see Polyclonal
0.0011759355 ABIN1954170 ELISA WB APC Rabbit IgG Log in to see Polyclonal
0.0011759355 ABIN1956598 ELISA WB FITC Rabbit IgG Log in to see Polyclonal
0.0011759355 ABIN1955412 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
0.0011759355 ABIN1958941 ELISA WB PE Rabbit IgG Log in to see Polyclonal
0.0011759355 ABIN1964393 IHC ELISA WB APC Rabbit IgG C-Term Log in to see Polyclonal
0.0011759355 ABIN1962014 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term Log in to see Polyclonal
0.0011759355 ABIN1100816 WB Rabbit C-Term Log in to see Polyclonal
0.0011759355 ABIN1989536 ELISA WB Mouse IgG2a kappa AA 1-431, full length Log in to see 3E9

Similar anti-CLASP2 Antibodies

Application / Reactivity Human
ELISA 49 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 28 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 56 Antibodies

General

Antigen Cytoplasmic Linker Associated Protein 2 (CLASP2) Antibodies
Epitope C-Term
(24), (23), (8), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(83), (29), (20)
Host Rabbit
(81), (8), (2)
Clonality
Polyclonal
Conjugate This CLASP2 antibody is un-conjugated
(7), (7), (7), (6), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(61), (49), (28), (11), (6), (6), (4), (2), (2), (1)
Pubmed 3 references available
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Product Details anti-CLASP2 Antibody

Target Details CLASP2 Application Details Handling References for anti-CLASP2 Antibody (ABIN359357) Images
Purification Protein A Chromatography followed by peptide Affinity Purification.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CLASP2.
Isotype Ig

Target Details CLASP2

Product Details anti-CLASP2 Antibody Application Details Handling References for anti-CLASP2 Antibody (ABIN359357) Images back to top
Antigen
Alternative Name CLASP2 (CLASP2 Antibody Abstract)
Background Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.Synonyms: CLIP-associating protein 2, Cytoplasmic linker-associated protein 2, KIAA0627, Protein Orbit homolog 2, hOrbit2
Gene ID 9606
UniProt O75122
Pathways Microtubule Dynamics

Application Details

Product Details anti-CLASP2 Antibody Target Details CLASP2 Handling References for anti-CLASP2 Antibody (ABIN359357) Images back to top
Application Notes ELISA: 1/1,000Western blotting: 1/100-1/500Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CLASP2 Antibody Target Details CLASP2 Application Details References for anti-CLASP2 Antibody (ABIN359357) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-CLASP2 Antibody (ABIN359357)

Product Details anti-CLASP2 Antibody Target Details CLASP2 Application Details Handling Images back to top
Background publications

Pereira, Pereira, Maia, Drabek, Sayas, Hergert, Lince-Faria, Matos, Duque, Stepanova, Rieder, Earnshaw, Galjart, Maiato: "Mammalian CLASP1 and CLASP2 cooperate to ensure mitotic fidelity by regulating spindle and kinetochore function." in: Molecular biology of the cell, Vol. 17, Issue 10, pp. 4526-42, 2006

Mimori-Kiyosue, Grigoriev, Sasaki, Matsui, Akhmanova, Tsukita, Vorobjev: "Mammalian CLASPs are required for mitotic spindle organization and kinetochore alignment." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 11, Issue 8, pp. 845-57, 2006

Lansbergen, Grigoriev, Mimori-Kiyosue, Ohtsuka, Higa, Kitajima, Demmers, Galjart, Houtsmuller, Grosveld, Akhmanova: "CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta." in: Developmental cell, Vol. 11, Issue 1, pp. 21-32, 2006

Images

Product Details anti-CLASP2 Antibody Target Details CLASP2 Application Details Handling References for anti-CLASP2 Antibody (ABIN359357) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CLASP2 antibody (Cytoplasmic Linker Associated Protein 2) (C-Term) (ABIN359357) Figure 1. Formalin-fixed and paraffin-embedded human Hepatocellular ...
Western Blotting (WB) image for anti-CLASP2 antibody (Cytoplasmic Linker Associated Protein 2) (C-Term) (ABIN359357) Figure 1. Western blot analysis of anti-CLASP2 Antibody (C-term) in ...