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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||57 Antibodies||15 Antibodies||8 Antibodies|
|RNA Interference (RNAi)||1 Antibodies|
|Immunoprecipitation (IP)||11 Antibodies||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||6 Antibodies||4 Antibodies||2 Antibodies|
|Immunohistochemistry (IHC)||7 Antibodies||4 Antibodies||4 Antibodies|
|Immunofluorescence (IF)||23 Antibodies||11 Antibodies||3 Antibodies|
|Immunocytochemistry (ICC)||8 Antibodies||1 Antibodies||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Antigen||Kinesin Family Member 2C (KIF2C) Antibodies|
|Epitope||AA 531-725 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This KIF2C antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-KIF2C AntibodyTarget Details KIF2C Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Kinesin-like protein KIF2C(KIF2C) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: kinesin family member 2C
Protein Name: Kinesin-like protein KIF2C
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived human MCAK recombinant protein (Position: G531-Q725). Human MCAK shares 87% amino acid (aa) sequence identity with both mouse and rat MCAK.|
Target Details KIF2CProduct Details anti-KIF2C Antibody Application Details Handling Images back to top
|Alternative Name||KIF2C (KIF2C Antibody Abstract)|
|Background||Kinesin-like protein KIF2C is a protein that in humans is encoded by the KIF2C gene. It is mapped to 1p34.1. The protein encoded by this gene is a member of kinesin-like protein family. Most proteins of this family are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein acts to regulate microtubule dynamics in cells and is important for anaphase chromosome segregation and may be required to coordinate the onset of sister centromere separation. KIF2C uses microtubule depolymerizing activity to correct improper microtubule attachments at kinetochores.|
Application DetailsProduct Details anti-KIF2C Antibody Target Details KIF2C Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, The detection limit for MCAK is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-KIF2C Antibody Target Details KIF2C Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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