|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||5 Antibodies||5 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies||2 Antibodies|
|Immunofluorescence (IF)||10 Antibodies||6 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||6 Antibodies||4 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||8 Antibodies||7 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies||3 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Microarray (MA)||1 Antibodies|
|Western Blotting (WB)||15 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies|
|Antigen||Microtubule-Associated Protein 1B (MAP1B) Antibodies|
|Reactivity||Cow (Bovine), Chicken, Cat (Feline), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAP1B antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC), Microarray (MA), Western Blotting (WB)
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Product Details anti-MAP1B AntibodyTarget Details MAP1B Application Details Handling Images
|Specificity||The antibody does not cross-react with other MAPs or tubulin.|
|Cross-Reactivity (Details)||May react with Hamster.|
|Immunogen||Rat brain microtubule associated proteins.|
Target Details MAP1BProduct Details anti-MAP1B Antibody Application Details Handling Images back to top
|Alternative Name||MAP1B (MAP1B Antibody Abstract)|
Alternate Names: Anti-DKFZp686E1099 antibody, Anti-DKFZp686F1345 antibody, Anti-FLJ38954antibody, Anti-FUTSCH antibody, Anti-MAP 1B antibody, Anti-MAP5 antibody,Anti-Microtubule-associated protein 1B antibody.
Gene Symbol: MAP1B
|Gene ID||4131, 17755, 29456|
Application DetailsProduct Details anti-MAP1B Antibody Target Details MAP1B Handling Images back to top
|Application Notes||Recommended dilutions: Microarray, Western Blot 1:500, Immunohistochemistry-Frozen 1:10-1:2000, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunohistochemistry 1:10-1:2000|
Protocol specific for MAP1B Antibody IHC protocol for cryostat sections (Protocol generously provided by Dr. Yimin Wang, Tissue Regeneration Therapeutics Inc )
1. Take out the cryostat section slides from -80C. Dry for few minutes at RT.
. Rinse slides with PBS to get rid of OCT. 3 min x 2, shake
. Fix slides with 1:1 methanol:acetone for 3 min
. Wash slides with PBS, 3 min x
. Circle tissue sections with PAP-Pen,
. Incubation with Normal Horse Serum: 5 % in 2 %BSA (in PBS-Tween20) for 20 minutes.
. MAP1B anti human antibody (1:200 in 2 % BSA 0.1 % Tween20). Incubate for 1.5 hours at 37C in a humid chamber.
. Wash slides with PBS-Tween (0.1 %), 5min x2The following steps are for IHC only
. Blocking of endogenous peroxidase: Incubation with 0.3 % H2O2 for 10 minutes.
. Wash PBS-Tween (0.1 %), 3 min x
. Incubation with secondary antibody (1:200 with PBS, anti mouse-bio, in AK-5002 kit) for 45 minutes at 37C in a humid chamber.
. Wash slides with PBS-Tween (0.1 %) 5min x
. Incubate with VECTAINABC reagent solution (bottle C, Cat No: PK6100, contains Avidin-HRP) for 30 minutes at RT in the dark.(For solution C: 10ml PBS + 2 drops of A and 2 drops of B, kept in 4C with dark)Prewarm at RT for 10min before use and drop 2 drops to the sections.
. Wash slides with PBS-Tween (0.1 %) 5min x
. Incubation with peroxidase substrate (Vector Nova Red, SK-4800) until desired stain intensity develops. Usually 1 to 2 minutes.Prepare the substrate immediately before use: can be used for > 1week.- Add 3 drops of Reagent 1 to 5 mL of DDW, mix well- Add 2 drops of Reagent 2 and mix well- Add 2 drops of Reagent 3 and mix well- Add 2 drops of Hydrogen Peroxide Solution and mix well
. Wash slides with tap water for 5 minutes.
. Hematoxylin nuclear staining: (Filter solution when first use, HE staining Jar,2nd one). Stain slides for 5 seconds at room temperature, then carefully rinse for 3 minutes under running cold tap water,
. Mount the slide with cc mount (Sigma, C9368, 30ml)
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP1B Antibody Target Details MAP1B Application Details Images back to top
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|