We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||10 Antibodies||52 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies||4 Antibodies|
|Immunofluorescence (IF)||2 Antibodies||17 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||10 Antibodies||10 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||21 Antibodies||21 Antibodies|
|Immunohistochemistry (IHC)||20 Antibodies||45 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||9 Antibodies|
|Western Blotting (WB)||39 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Flow Cytometry (FACS)||4 Antibodies|
|Antigen||Ras Homolog Gene Family, Member A (RHOA) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This RHOA antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-RHOA AntibodyTarget Details RHOA Application Details Handling Images
|Specificity||This antibody detects endogenous levels of Rho A protein. (region surrounding Arg182)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details RHOAProduct Details anti-RHOA Antibody Application Details Handling Images back to top
|Alternative Name||RhoA (RHOA Antibody Abstract)|
|Background||The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1 (A and B), Rap 2, R-Ras and Ral (A and B), exhibit approximately 50 % amino acid homology to Ras. The three mammalian Rho proteins (A, B and C) are approximately 30 % homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy terminal CAAX sequence (C, cysteine, A, aliphatic amino acid, X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.Synonyms: ARH12, ARHA, RHO12, Rho cDNA clone 12, Transforming protein RhoA|
|Molecular Weight||approx. 22 kDa|
|Research Area||Cancer, Cytoskeleton, Microfilaments|
|Pathways||Microtubule Dynamics, WNT Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-RHOA Antibody Target Details RHOA Handling Images back to top
ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-RHOA Antibody Target Details RHOA Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
ImagesProduct Details anti-RHOA Antibody Target Details RHOA Application Details Handling back to top