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anti-Human RHOA antibody for Immunoprecipitation

Recommended RHOA Antibody (supplied by: Log in to see )

Antigen
Ras Homolog Gene Family, Member A (RHOA) Antibodies
  • arh12
  • ARH12
  • Arha
  • arha
  • ARHA
  • Arha1
  • Arha2
  • arha2
  • RHO1
  • RHO12
  • rho12
  • rhoa
  • Rhoa
  • RHOH12
  • rhoh12
  • wu:fa66g04
  • wu:fb26g07
  • xrhoa
  • zgc:66058
  • zgc:85805
Epitope
AA 1-193
17
15
11
8
5
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
133
58
53
4
3
1
1
1
1
Host
Rabbit
91
37
3
2
Clonality
Polyclonal
Conjugate
This RHOA antibody is un-conjugated
9
7
7
2
2
2
2
2
2
2
2
1
1
Minimum Detection Limit
0.00975 ng/well
Application
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
97
51
42
21
19
15
10
4
3
2
2
1
1
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN1999717
$ 218.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.5013809 ABIN2649374 IP WB Rabbit Log in to see

Similar anti-RHOA Antibodies

Application / Reactivity Human
ELISA 52 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 21 Antibodies
Immunohistochemistry (IHC) 45 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
Western Blotting (WB) 101 Antibodies

General

Antigen Ras Homolog Gene Family, Member A (RHOA) Antibodies
Epitope AA 1-193
(17), (15), (11), (8), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(133), (58), (53), (4), (3), (1), (1), (1), (1)
Host Rabbit
(91), (37), (3), (2)
Clonality
Polyclonal
Conjugate This RHOA antibody is un-conjugated
(9), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Minimum Detection Limit 0.00975 ng/well
Application Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(97), (51), (42), (21), (19), (15), (10), (4), (3), (2), (2), (1), (1)
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Product Details anti-RHOA Antibody

Target Details RHOA Application Details Handling Images
Specificity Human RhoA
Characteristics Produced in rabbits immunized with purified, recombinant Human RhoA (rh RhoA, P61586-1, Met 1-Leu 193). RhoA specific IgG was purified by Human RhoA affinity chromatography.
Purification Affinity Purified
Sterility 0.2 μm filtered
Immunogen Recombinant Human RhoA protein
Isotype IgG

Target Details RHOA

Product Details anti-RHOA Antibody Application Details Handling Images back to top
Antigen
Alternative Name RHOA (RHOA Antibody Abstract)
Background Transforming protein RhoA, also known as Rho cDNA clone 12, Ras homolog gene family member A, RHOA and ARH12, is a cell membrane and cytoplasm protein which belongs to thesmall GTPase superfamily and Rho family. The Rho family of small GTPases plays a key role in the dynamic regulation of the actin cytoskeleton that underlies various important cellular functions such as shape changes, migration, and polarity. RHOA / ARH12 is part of a larger family of related proteins known as the Ras superfamily, proteins involved in the regulation and timing of cell division. RHOA / ARH12 is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila)). RHOA / ARH12 regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RHOA / ARH12 serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RHOA / ARH12 may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP.
UniProt P61586-1
Pathways Microtubule Dynamics, WNT Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RHOA Antibody Target Details RHOA Handling Images back to top
Application Notes Western blot - WB: 5-10 μg/mL Direct ELISA - This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human RHOA. The detection limit for Human RHOA is approximately 0.00975 ng/well.
Restrictions For Research Use only

Handling

Product Details anti-RHOA Antibody Target Details RHOA Application Details Images back to top
Buffer PBS with 5% trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C/-20 °C/-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -70°C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.
Expiry Date 12 months

Images

Product Details anti-RHOA Antibody Target Details RHOA Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-RHOA antibody (Ras Homolog Gene Family, Member A) (AA 1-193) (ABIN1999717) anti-Ras Homolog Gene Family, Member A (RHOA) (AA 1-193) antibody
Immunoprecipitation (IP) image for anti-RHOA antibody (Ras Homolog Gene Family, Member A) (AA 1-193) (ABIN1999717) anti-Ras Homolog Gene Family, Member A (RHOA) (AA 1-193) antibody (Image 2)