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anti-Mouse (Murine) RHOA antibody for Western Blotting

Recommended RHOA Antibody (supplied by: Log in to see )

Antigen
Ras Homolog Gene Family, Member A (RHOA) Antibodies
  • arh12
  • ARH12
  • Arha
  • arha
  • ARHA
  • Arha1
  • Arha2
  • arha2
  • RHO1
  • RHO12
  • rho12
  • rhoa
  • Rhoa
  • RHOH12
  • rhoh12
  • wu:fa66g04
  • wu:fb26g07
  • xrhoa
  • zgc:66058
  • zgc:85805
Epitope
Middle Region
18
15
11
8
5
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
137
60
53
3
2
1
Host
Rabbit
93
39
3
2
Clonality
Polyclonal
Conjugate
This RHOA antibody is un-conjugated
9
7
7
2
2
2
2
2
2
2
2
1
1
Application
Western Blotting (WB)
101
52
43
22
21
18
10
5
4
4
3
2
1
Supplier
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Catalog No. ABIN2787722
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13398378 ABIN758242 IF (p) IHC (p) WB Rabbit IgG pSer188 Log in to see Polyclonal 1
0.13398378 ABIN1532406 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13398378 ABIN2854917 WB Rabbit IgG Center Log in to see Polyclonal
0.13398378 ABIN351036 IHC WB Rabbit AA 100-150 Log in to see Polyclonal 5
0.13398378 ABIN1874602 WB Rabbit IgG Log in to see Polyclonal
0.13398378 ABIN1585792 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.13398378 ABIN2499810 IHC ELISA WB Rabbit IgG Ser188 Log in to see Polyclonal
0.13398378 ABIN3020849 WB Rabbit IgG Log in to see Polyclonal
0.13398378 ABIN3186754 ELISA IHC WB Rabbit IgG Ser155 Log in to see Polyclonal
0.13398378 ABIN2403550 IHC WB Rabbit IgG Log in to see Polyclonal
0.13398378 ABIN2476359 IF IHC (p) WB Mouse IgG1 AA 1-194 Log in to see 1B12
0.13398378 ABIN2425541 ELISA WB Rabbit IgG Log in to see Polyclonal
0.13398378 ABIN2431007 ELISA WB Rabbit IgG Log in to see Polyclonal
0.13398378 ABIN2431031 ELISA WB Rabbit IgG Log in to see Polyclonal
0.13398378 ABIN1844954 WB Rabbit pSer188 Log in to see Polyclonal
0.13398378 ABIN1882298 IHC WB Rabbit Log in to see Polyclonal
0.13398378 ABIN1844956 IHC WB Rabbit Log in to see Polyclonal
0.0011731827 ABIN738846 IF (cc) IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
0.0011731827 ABIN272117 IHC (p) WB Rabbit Log in to see Polyclonal
0.0011731827 ABIN351038 IHC WB Sheep AA 100-150 Log in to see Polyclonal 5

Top referenced anti-RHOA antibody for Western Blotting

Similar anti-RHOA Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Chicken Cow (Bovine) Xenopus laevis Zebrafish (Danio rerio)
Western Blotting (WB) 105 Antibodies 42 Antibodies 35 Antibodies 4 Antibodies 1 Antibodies 3 Antibodies 1 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 25 Antibodies 12 Antibodies 9 Antibodies
Immunohistochemistry (IHC) 46 Antibodies 21 Antibodies 17 Antibodies 2 Antibodies 2 Antibodies

General

Antigen Ras Homolog Gene Family, Member A (RHOA) Antibodies
Epitope Middle Region
(18), (15), (11), (8), (5), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(137), (60), (53), (3), (2), (1)
Host Rabbit
(93), (39), (3), (2)
Clonality
Polyclonal
Conjugate This RHOA antibody is un-conjugated
(9), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Western Blotting (WB)
(101), (52), (43), (22), (21), (18), (10), (5), (4), (4), (3), (2), (1)
Pubmed 1 reference available
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Product Details anti-RHOA Antibody

Target Details RHOA Application Details Handling References for anti-RHOA Antibody (ABIN2787722) Images
Blocking Peptide Blocking peptide for this product available: ABIN982960
Sequence GRDMANRIGA FGYMECSAKT KDGVREVFEM ATRAALQARR GKKKSGCLVL
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Human : 100 %, Mouse : 100 %, Rat : 100 %, Sheep : 100 %, Zebrafish : 100 %
Characteristics This is a rabbit polyclonal antibody against RHOA. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human RHOA

Target Details RHOA

Product Details anti-RHOA Antibody Application Details Handling References for anti-RHOA Antibody (ABIN2787722) Images back to top
Antigen
Alternative Name RHOA (RHOA Antibody Abstract)
Background RHOA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RHOA serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. It may be an activator of PLCE1. RHOA is activated by ARHGEF2, which promotes the exchange of GDP for GTP.
Molecular Weight 21 kDa
Gene ID 387
NCBI Accession NM_001664, NP_001655
UniProt P61586
Pathways Microtubule Dynamics, WNT Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RHOA Antibody Target Details RHOA Handling References for anti-RHOA Antibody (ABIN2787722) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 193 AA

Restrictions For Research Use only

Handling

Product Details anti-RHOA Antibody Target Details RHOA Application Details References for anti-RHOA Antibody (ABIN2787722) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-RHOA Antibody (ABIN2787722)

Product Details anti-RHOA Antibody Target Details RHOA Application Details Handling Images back to top
Background publications

Kakinuma, Roy, Zhu, Wang, Kiyama: "Kank regulates RhoA-dependent formation of actin stress fibers and cell migration via 14-3-3 in PI3K-Akt signaling." in: The Journal of cell biology, Vol. 181, Issue 3, pp. 537-49, 2008

Images

Product Details anti-RHOA Antibody Target Details RHOA Application Details Handling References for anti-RHOA Antibody (ABIN2787722) back to top
Supplier Images
Western Blotting (WB) image for anti-RHOA antibody (Ras Homolog Gene Family, Member A) (Middle Region) (ABIN2787722) WB Suggested Anti-RHOA
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.2500