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anti-Human ROCK1 antibody for Western Blotting

Recommended ROCK1 Antibody (supplied by: Log in to see )

Antigen
rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) Antibodies
  • 1110055K06Rik
  • CG9774
  • Dmel\\CG9774
  • DRhk
  • dRok
  • drok
  • Drok
  • DROK
  • DRok
  • dROK
  • LOC100229794
  • P160ROCK
  • P160Rock
  • p160ROCK
  • Rhk
  • RhoK
  • ROCK
  • Rock
  • ROCK-1
  • ROCK-I
  • Rock-I
  • ROCK1
  • ROK
  • Rok
  • ROKalpha/beta
Epitope
N-Term
25
24
13
11
9
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
114
72
70
3
3
2
2
2
1
Host
Rabbit
102
17
Clonality
Polyclonal
Conjugate
This ROCK1 antibody is un-conjugated
4
3
3
3
3
3
3
3
3
3
3
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
81
32
29
19
14
13
9
4
2
1
1
1
Supplier
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Catalog No. ABIN783446
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN969563 ELISA WB Mouse IgG1 Log in to see 1H4 2
0.12303223 ABIN968386 IF WB Mouse IgG1 AA 906-1012 Log in to see 46-ROCK 5
0.12303223 ABIN968387 IF WB Mouse IgG1 AA 906-1012 Log in to see 46-ROCK 5
0.12303223 ABIN562671 IF ELISA WB Mouse IgG1 kappa AA 401-510, partial Log in to see 2E2 2
0.12303223 ABIN258448 ICC FACS IHC (fro) IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see EP786Y
0.12303223 ABIN2706968 IC IF WB Rabbit Center Log in to see Polyclonal
0.12303223 ABIN393382 IF WB Mouse IgG1 kappa AA 401-511 Log in to see 2E2
0.12303223 ABIN152055 ICC IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal
0.12303223 ABIN1874629 WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN948412 ELISA WB Mouse IgG1 kappa AA 401-510, partial Log in to see 2E2
0.12303223 ABIN2499974 ELISA WB Rabbit N-Term Log in to see Polyclonal
0.12303223 ABIN2499976 IHC (fp) IF WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2856887 ICC IF IP IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.12303223 ABIN1031544 ELISA WB Rabbit N-Term Log in to see Polyclonal
0.12303223 ABIN3186829 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
0.12303223 ABIN1846125 ELISA WB Mouse IgG1 Log in to see
0.12303223 ABIN1844979 WB Rabbit Log in to see Polyclonal
0.12303223 ABIN3015089 WB Rabbit Log in to see Polyclonal
0.0011806369 ABIN738636 IF (p) IHC (p) WB Rabbit IgG AA 980-1030 Log in to see Polyclonal 1
0.0011806369 ABIN707771 IF (p) IHC (p) WB Rabbit IgG pThr455, pSer456 Log in to see Polyclonal 2

Top referenced anti-ROCK1 antibody for Western Blotting

Similar anti-ROCK1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 81 Antibodies 38 Antibodies 37 Antibodies
Immunoprecipitation (IP) 11 Antibodies 3 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies 13 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 17 Antibodies 7 Antibodies 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 32 Antibodies 32 Antibodies

General

Antigen rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) Antibodies
Epitope N-Term
(25), (24), (13), (11), (9), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(114), (72), (70), (3), (3), (2), (2), (2), (1)
Host Rabbit
(102), (17)
Clonality
Polyclonal
Conjugate This ROCK1 antibody is un-conjugated
(4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(81), (32), (29), (19), (14), (13), (9), (4), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-ROCK1 Antibody

Target Details ROCK1 Application Details Handling References for anti-ROCK1 Antibody (ABIN783446) Images
Specificity This antibody detects ROCK1 at N-term. ROCK1 antibody is predicted to not cross-react with other ROCK protein family members.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Affinity chromatography purified via peptide column
Immunogen 15 amino acid peptide near the amino terminus of human ROCK1

Target Details ROCK1

Product Details anti-ROCK1 Antibody Application Details Handling References for anti-ROCK1 Antibody (ABIN783446) Images back to top
Antigen
Alternative Name ROCK1 (ROCK1 Antibody Abstract)
Background ROCK1 is a member of the AGC serine/threonine protein kinase family that regulates the assembly of the actin cytoskeleton. ROCK1 contains a protein kinase domain, a coiled-coil region and a zinc finger region and appears to be present as a dimer. ROCK1, like its isozyme ROCK2, is a downstream target of the small G-protein Rho and plays a role in smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis, and mediates the cellular morphological changes during apoptosis.Synonyms: NY-REN-35, ROCK-1, Renal carcinoma antigen NY-REN-35, Rho-associated, Rho-associated protein kinase 1, coiled-coil-containing protein kinase 1, p160ROCK
Gene ID 6093
NCBI Accession NP_005397
UniProt Q13464
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments, Apoptosis/Necrosis, Signaling
Pathways Microtubule Dynamics, WNT Signaling

Application Details

Product Details anti-ROCK1 Antibody Target Details ROCK1 Handling References for anti-ROCK1 Antibody (ABIN783446) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details References for anti-ROCK1 Antibody (ABIN783446) Images back to top
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer.

References for anti-ROCK1 Antibody (ABIN783446)

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Handling Images back to top
Background publications

Takahashi, Tuiki, Saya, Kaibuchi: "Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24." in: Genomics, Vol. 55, Issue 2, pp. 235-7, 1999

Ishizaki, Maekawa, Fujisawa, Okawa, Iwamatsu, Fujita, Watanabe, Saito, Kakizuka, Morii, Narumiya: "The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase." in: The EMBO journal, Vol. 15, Issue 8, pp. 1885-93, 1996

Images

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Handling References for anti-ROCK1 Antibody (ABIN783446) back to top
Supplier Images
Western Blotting (WB) image for anti-ROCK1 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 1) (N-Term) (ABIN783446) Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 antibody at 1 μg/ml in (...