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anti-Mouse (Murine) ROCK1 antibody for Western Blotting

Recommended ROCK1 Antibody (supplied by: Log in to see )

Antigen
rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) Antibodies
  • LOC100229794
  • CG9774
  • DROK
  • DRhk
  • DRok
  • Dmel\\CG9774
  • Drok
  • ROCK
  • ROCK-1
  • ROCK1
  • ROK
  • ROKalpha/beta
  • Rhk
  • RhoK
  • Rock
  • Rok
  • dROK
  • dRok
  • drok
  • 1110055K06Rik
  • Rock-I
  • P160Rock
  • ROCK-I
  • P160ROCK
  • p160ROCK
  • Rho-associated, coiled-coil containing protein kinase 1
  • Rho-kinase
  • Rho-associated coiled-coil containing protein kinase 1
  • ROCK1
  • LOC100229794
  • rok
  • Rock1
  • rock1
Epitope
N-Term
27
25
24
20
13
13
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
144
99
95
21
19
19
3
2
2
1
1
Host
Rabbit
107
40
Clonality
Polyclonal
Conjugate
This ROCK1 antibody is un-conjugated
4
4
4
4
4
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
108
32
32
31
23
22
19
11
2
1
1
1
1
Supplier
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Catalog No. ABIN783446
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9590793 ABIN252549 ICC IF WB Mouse IgG C-Term, cleaved Log in to see 154C1465 4
2.9590793 ABIN152055 ICC IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal 2
2.9590793 ABIN968387 IF WB Mouse IgG1 AA 906-1012 Log in to see 46-ROCK 5
2.9590793 ABIN968386 IF WB Mouse IgG1 AA 906-1012 Log in to see 46-ROCK 5
2.9590793 ABIN2706968 IC IF WB Rabbit Center Log in to see Polyclonal
2.9590793 ABIN3186829 ELISA ICC IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
2.9590793 ABIN4350895 WB Rabbit IgG Log in to see 12M03
2.9590793 ABIN1874629 IHC WB Rabbit IgG Log in to see Polyclonal
2.9590793 ABIN4350897 ELISA ICC IF WB Rabbit N-Term Log in to see Polyclonal
2.9590793 ABIN2499974 ELISA WB Rabbit N-Term Log in to see Polyclonal
2.9590793 ABIN4350919 WB Mouse IgG1 N-Term Log in to see 261
2.9590793 ABIN4350920 WB Mouse IgG2b N-Term Log in to see 464
2.9590793 ABIN4350918 ICC IF WB Mouse IgG C-Term, cleaved Log in to see 154C1465
2.9590793 ABIN2856887 ICC IF IP IHC (p) WB Rabbit N-Term Log in to see Polyclonal
2.9590793 ABIN4350898 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
2.9590793 ABIN1031544 ELISA WB Rabbit N-Term Log in to see Polyclonal
2.9590793 ABIN2737481 WB Rabbit IgG Log in to see Polyclonal
2.9590793 ABIN1844979 WB Rabbit Log in to see Polyclonal
1 ABIN738636 IF (p) IHC (p) WB Rabbit IgG AA 980-1030 Log in to see Polyclonal 1
1 ABIN707771 IF (p) IHC (p) WB Rabbit IgG pThr455, pSer456 Log in to see Polyclonal 2

Top referenced anti-ROCK1 antibody for Western Blotting

Similar anti-ROCK1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 107 Antibodies 64 Antibodies 61 Antibodies
Immunoprecipitation (IP) 11 Antibodies 4 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies 14 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 19 Antibodies 10 Antibodies 9 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 32 Antibodies 32 Antibodies

General

Antigen rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) Antibodies
Epitope N-Term
(27), (25), (24), (20), (13), (13), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(144), (99), (95), (21), (19), (19), (3), (2), (2), (1), (1)
Host Rabbit
(107), (40)
Clonality
Polyclonal
Conjugate This ROCK1 antibody is un-conjugated
(4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(108), (32), (32), (31), (23), (22), (19), (11), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-ROCK1 Antibody

Target Details ROCK1 Application Details Handling References for anti-ROCK1 Antibody (ABIN783446) Images
Specificity This antibody detects ROCK1 at N-term. ROCK1 antibody is predicted to not cross-react with other ROCK protein family members.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Affinity chromatography purified via peptide column
Immunogen 15 amino acid peptide near the amino terminus of human ROCK1

Target Details ROCK1

Product Details anti-ROCK1 Antibody Application Details Handling References for anti-ROCK1 Antibody (ABIN783446) Images back to top
Antigen
Alternative Name ROCK1 (ROCK1 Antibody Abstract)
Background ROCK1 is a member of the AGC serine/threonine protein kinase family that regulates the assembly of the actin cytoskeleton. ROCK1 contains a protein kinase domain, a coiled-coil region and a zinc finger region and appears to be present as a dimer. ROCK1, like its isozyme ROCK2, is a downstream target of the small G-protein Rho and plays a role in smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis, and mediates the cellular morphological changes during apoptosis.Synonyms: NY-REN-35, ROCK-1, Renal carcinoma antigen NY-REN-35, Rho-associated, Rho-associated protein kinase 1, coiled-coil-containing protein kinase 1, p160ROCK
Gene ID 6093
NCBI Accession NP_005397
UniProt Q13464
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments, Apoptosis/Necrosis, Signaling
Pathways Microtubule Dynamics, WNT Signaling

Application Details

Product Details anti-ROCK1 Antibody Target Details ROCK1 Handling References for anti-ROCK1 Antibody (ABIN783446) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details References for anti-ROCK1 Antibody (ABIN783446) Images back to top
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer.

References for anti-ROCK1 Antibody (ABIN783446)

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Handling Images back to top
Background publications

Takahashi, Tuiki, Saya, Kaibuchi: "Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24." in: Genomics, Vol. 55, Issue 2, pp. 235-7, 1999

Ishizaki, Maekawa, Fujisawa, Okawa, Iwamatsu, Fujita, Watanabe, Saito, Kakizuka, Morii, Narumiya: "The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase." in: The EMBO journal, Vol. 15, Issue 8, pp. 1885-93, 1996

Images

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Handling References for anti-ROCK1 Antibody (ABIN783446) back to top
Supplier Images
Western Blotting (WB) image for anti-ROCK1 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 1) (N-Term) (ABIN783446) Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 antibody at 1 μg/ml in (...