|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Cow (Bovine)||Dog (Canine)||Chicken||Fruit Fly (Drosophila melanogaster)||Xenopus laevis|
|Immunofluorescence (fixed cells) (IF/ICC)||3 Antibodies||2 Antibodies||2 Antibodies|
|Western Blotting (WB)||125 Antibodies||68 Antibodies||73 Antibodies||6 Antibodies||4 Antibodies||1 Antibodies||1 Antibodies||1 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies||1 Antibodies||1 Antibodies||1 Antibodies|
|Immunoprecipitation (IP)||28 Antibodies||16 Antibodies||20 Antibodies||2 Antibodies||2 Antibodies|
|Antigen||rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies|
|Reactivity||Chicken, Cow (Bovine), Dog (Canine), Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis Alternatives|
|Conjugate||This ROCK2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Product Details anti-ROCK2 AntibodyTarget Details ROCK2 Application Details Handling Images
|Specificity||This phospho-specific polyclonal antibody reacts with Human ROCK-2 pTyr256 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Expect a band approximately 164 kDa in size corresponding to phosphorylated ROCK-2|
Species reactivity (expected):Mouse, Rat, Chicken, Canine, Bovine, Xenopus, Drosophila.
Species reactivity (tested):Human.
|Characteristics||Concentration: 1.1 mg/mL (by UV absorbance at 280 nm)|
|Immunogen||Synthetic peptide corresponding to residues surrounding Y256 of human ROCK-2|
Target Details ROCK2Product Details anti-ROCK2 Antibody Application Details Handling Images back to top
|Alternative Name||ROCK2 (ROCK2 Antibody Abstract)|
|Background||Progression of tumors to invasive and metastatic forms requires that tumor cells undergo dramatic morphological changes, a process regulated by Rho GTPases. Increased levels of RhoA or Rh °C, as well as the Rho effector proteins ROCK-1 or ROCK-2, were observed in various human carcinomas. In vivo studies have also shown that ROCK inhibition reduced the invasiveness of several tumor cell lines. It is suggested that Rho and ROCK signaling contribute to the morphological changes and metastatic behavior of some tumor cells. Thus, elevated Rho and ROCK expression is associated with tumorigenesis, particularly during the progression to invasive and metastatic phenotypes. Phosphorylation of certain residues in ROCK-2 may be critical for its kinase activity.Synonyms: KIAA0619, ROCK-2, Rho kinase 2, Rho-associated, Rho-associated protein kinase 2, coiled-coil-containing protein kinase 2, p164ROCK-2|
|Research Area||Apoptosis/Necrosis, Cancer|
|Pathways||Microtubule Dynamics, WNT Signaling, Tube Formation|
Application DetailsProduct Details anti-ROCK2 Antibody Target Details ROCK2 Handling Images back to top
ELISA: 1/2,000-1/10,000. Western Blot: 1/200-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Images back to top
|Buffer||0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-28 °C for longer.|