anti-Dog (Canine) ROCK2 antibody for Western Blotting

Recommended ROCK2 Antibody (supplied by: Log in to see )

Antigen
rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies
  • rocka-a
  • xROK
  • rock2
  • rok2
  • ROCK2
  • B230113H15Rik
  • ROKalpha
  • Rho-kinase
  • Rock-II
  • Rock2m
  • mKIAA0619
  • ROCK-II
  • ROK
  • Rho-associated, coiled-coil containing protein kinase 2
  • rho-associated, coiled-coil containing protein kinase 2a
  • Rho-associated coiled-coil containing protein kinase 2
  • Rho-associated coiled-coil containing protein kinase 2-like
  • rock2
  • rock2a
  • ROCK2
  • Rock2
  • LOC100346837
Epitope
AA 567-718
16
15
15
12
6
6
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
169
117
112
9
5
4
3
3
2
2
1
1
1
1
1
1
Host
Mouse
149
20
1
Clonality
Monoclonal
Conjugate
This ROCK2 antibody is un-conjugated
5
5
4
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
125
44
40
39
33
27
24
11
3
3
2
Options
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Catalog No. ABIN968070
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1581392 ABIN968071 IF IP IHC WB Mouse IgG1 AA 567-718 Log in to see 21-ROCK 5
1 ABIN5551814 EIA WB Rabbit IgG pTyr256 Log in to see Polyclonal
1 ABIN2788813 IHC WB Rabbit Middle Region Log in to see Polyclonal

Top referenced anti-ROCK2 antibody for Western Blotting

Similar anti-ROCK2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 22 Antibodies 22 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 3 Antibodies
Immunofluorescence (IF) 10 Antibodies 10 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies 40 Antibodies
Immunohistochemistry (IHC) 20 Antibodies 20 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies 28 Antibodies
Immunoprecipitation (IP) 16 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 68 Antibodies

General

Antigen rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies
Epitope AA 567-718
(16), (15), (15), (12), (6), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(169), (117), (112), (9), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(149), (20), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ROCK2 antibody is un-conjugated
(5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(125), (44), (40), (39), (33), (27), (24), (11), (3), (3), (2)
Pubmed 5 references available
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Product Details anti-ROCK2 Antibody

Target Details ROCK2 Application Details Handling ProductDetails: References for anti-ROCK2 antibody (ABIN968070) Images
Cross-Reactivity Mouse (Murine), Dog (Canine), Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Rat ROKalpha aa. 567-718
Clone 21-ROCK
Isotype IgG1

Target Details ROCK2

Product Details anti-ROCK2 Antibody Application Details Handling ProductDetails: References for anti-ROCK2 antibody (ABIN968070) Images back to top
Antigen
Alternative Name ROCK-II (ROCK2 Antibody Abstract)
Background Activity of the GTP-binding proteins is regulated by GAPs, that accelerate binding, and GTPases, that enhance the rate of GTP hydrolysis (Ras, Rho, cdc42Hs, and Rac). The isoforms of Rho, a small GTP-binding protein, regulate cellular processes such as the formation of the stress fibers, lamelipodia, and filopodia. ROKalpha (RhoA-binding kinase) is a Ser/Thr protein kinase that interects with the GTP-binding form of RhoA. Like RhoA, ROKalpha localizes at the cellular margins and colocalizes with acti filaments. The ROKalpha gene encodes a protein of 1302 amino acids with homology to the human myotonic kinase. Although ROKalpha binds to GTP-RhoA, it is not yet clear whether this interaction induces the kinase activity of ROKalpha.
Synonyms: ROKalpha
Molecular Weight 180 kDa
Pathways Microtubule Dynamics, WNT Signaling, Tube Formation

Application Details

Product Details anti-ROCK2 Antibody Target Details ROCK2 Handling ProductDetails: References for anti-ROCK2 antibody (ABIN968070) Images back to top
Restrictions For Research Use only

Handling

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details ProductDetails: References for anti-ROCK2 antibody (ABIN968070) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

ProductDetails: References for anti-ROCK2 antibody (ABIN968070)

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Handling Images back to top
Product cited in:

Begum, Sandu, Ito, Lohmann, Smolenski: "Active Rho kinase (ROK-alpha ) associates with insulin receptor substrate-1 and inhibits insulin signaling in vascular smooth muscle cells." in: The Journal of biological chemistry, Vol. 277, Issue 8, pp. 6214-22, 2002

Wang, Eto, Steers, Somlyo, Somlyo: "RhoA-mediated Ca2+ sensitization in erectile function." in: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 30614-21, 2002

Adachi, Vita, Sannohe, Stafford, Alam, Kayaba, Chihara: "The functional role of rho and rho-associated coiled-coil forming protein kinase in eotaxin signaling of eosinophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 8, pp. 4609-15, 2001

Sahai, Olson, Marshall: "Cross-talk between Ras and Rho signalling pathways in transformation favours proliferation and increased motility." in: The EMBO journal, Vol. 20, Issue 4, pp. 755-66, 2001

Leung, Manser, Tan, Lim: "A novel serine/threonine kinase binding the Ras-related RhoA GTPase which translocates the kinase to peripheral membranes." in: The Journal of biological chemistry, Vol. 270, Issue 49, pp. 29051-4, 1996

Images

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Handling ProductDetails: References for anti-ROCK2 antibody (ABIN968070) back to top
Supplier Images
Western Blotting (WB) image for anti-ROCK2 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 2) (AA 567-718) (ABIN968070) Western blot analysis of ROCK-II on RSV-3T3 lysate. Lane 1: 1:1000, lane 2: 1:2000, l...
 image for anti-ROCK2 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 2) (AA 567-718) (ABIN968070) MCF7