anti-Human ROCK2 antibody for Immunocytochemistry

Recommended ROCK2 Antibody (supplied by: Log in to see )

Antigen
rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies
  • rocka-a
  • xROK
  • rock2
  • rok2
  • ROCK2
  • B230113H15Rik
  • ROKalpha
  • Rho-kinase
  • Rock-II
  • Rock2m
  • mKIAA0619
  • ROCK-II
  • ROK
  • Rho-associated, coiled-coil containing protein kinase 2
  • rho-associated, coiled-coil containing protein kinase 2a
  • Rho-associated coiled-coil containing protein kinase 2
  • Rho-associated coiled-coil containing protein kinase 2-like
  • rock2
  • rock2a
  • ROCK2
  • Rock2
  • LOC100346837
Epitope
Center
16
15
15
12
6
6
5
5
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
172
118
112
9
5
5
3
3
2
2
1
1
1
1
1
1
Host
Rabbit
151
21
1
Clonality
Polyclonal
Conjugate
This ROCK2 antibody is un-conjugated
5
5
4
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
128
45
40
37
36
27
24
10
3
3
2
Options
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Catalog No. ABIN2855440
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4350921 ELISA ICC IF IHC WB Rabbit Center Log in to see Polyclonal
1 ABIN4350924 ICC IF IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN1931396 ICC ELISA WB FITC Mouse IgG2b AA 1279-1389 Log in to see
1 ABIN1931393 ICC ELISA WB Alkaline Phosphatase (AP) Mouse IgG2b AA 1279-1389 Log in to see
1 ABIN1931394 ICC ELISA WB APC Mouse IgG2b AA 1279-1389 Log in to see
1 ABIN1931395 ICC ELISA WB Biotin Mouse IgG2b AA 1279-1389 Log in to see
1 ABIN1931397 ICC ELISA WB PE Mouse IgG2b AA 1279-1389 Log in to see
1 ABIN5079377 ICC IF WB Rabbit IgG Log in to see Polyclonal
1 ABIN1931398 ICC ELISA WB HRP Mouse IgG2b AA 1279-1389 Log in to see
1 ABIN1870404 ICC IHC IP WB Rabbit IgG AA 904-1140 Log in to see Polyclonal

General

Antigen rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies
Epitope Center
(16), (15), (15), (12), (6), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(172), (118), (112), (9), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(151), (21), (1)
Clonality
Polyclonal
Conjugate This ROCK2 antibody is un-conjugated
(5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(128), (45), (40), (37), (36), (27), (24), (10), (3), (3), (2)
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Product Details anti-ROCK2 Antibody

Target Details ROCK2 Application Details Handling Images
Characteristics Rabbit Polyclonal antibody to ROCK2 (Rho-associated, coiled-coil containing protein kinase 2)
ROCK2 antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human ROCK2. The exact sequence is proprietary.
Isotype IgG

Target Details ROCK2

Product Details anti-ROCK2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ROCK2 (ROCK2 Antibody Abstract)
Background The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Cellular Localization: Cytoplasm (By similarity) , Cell membrane, Peripheral membrane protein (By similarity)
Molecular Weight 161 kDa
Gene ID 9475
Research Area Cancer, Apoptosis/Necrosis
Pathways Microtubule Dynamics, WNT Signaling, Tube Formation

Application Details

Product Details anti-ROCK2 Antibody Target Details ROCK2 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , HeLa , HepG2 , Rat2 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , A549 , H1299 , HCT116

Restrictions For Research Use only

Handling

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Images back to top
Format Liquid
Concentration 0.67 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (Center) antibody (ABIN2855440) WB Image ROCK2 antibody detects ROCK2 protein by western blot analysis. A. 30 μg A549...
Immunohistochemistry (IHC) image for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (Center) antibody (ABIN2855440) IHC-P Image ROCK2 antibody detects ROCK2 protein at cytosol on mouse kidney by immuno...
Immunofluorescence (IF) image for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (Center) antibody (ABIN2855440) ICC/IF Image ROCK2 antibody detects ROCK2 protein at cytoplasm by immunofluorescent a...
Western Blotting (WB) image for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (Center) antibody (ABIN2855440) WB Image ROCK2 antibody detects ROCK2 protein by western blot analysis. A. 30 μg Rat2...
Western Blotting (WB) image for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (Center) antibody (ABIN2855440) WB Image ROCK2 antibody detects ROCK2 protein by western blot analysis. A. 30 μg Neur...
Immunoprecipitation (IP) image for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (Center) antibody (ABIN2855440) IP Image Immunoprecipitation of ROCK2 protein from HeLa whole cell extracts using 5 μ...
Western Blotting (WB) image for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (Center) antibody (ABIN2855440) WB Image ROCK2 antibody detects ROCK2 protein by western blot analysis. Various whole...