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anti-Human ROCK2 antibody for Immunoprecipitation

Recommended ROCK2 Antibody (supplied by: Log in to see )

Antigen
rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies
  • B230113H15Rik
  • mKIAA0619
  • Rho-kinase
  • Rock-II
  • ROCK-II
  • rock2
  • ROCK2
  • Rock2m
  • rocka-a
  • ROK
  • rok2
  • ROKalpha
  • xROK
Epitope
Center
15
14
13
13
6
6
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
158
90
88
4
3
2
2
1
1
1
1
Host
Rabbit
137
21
1
Clonality
Polyclonal
Conjugate
This ROCK2 antibody is un-conjugated
5
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
118
45
38
34
30
23
19
11
3
1
1
Supplier
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Catalog No. ABIN2855440
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089556724 ABIN968071 IF IP IHC WB Mouse IgG1 AA 567-718 Log in to see 21-ROCK 5
0.00089556724 ABIN968070 IF IP IHC WB Mouse IgG1 AA 567-718 Log in to see 21-ROCK 5
0.00089556724 ABIN152024 IP WB Rabbit AA 1-100 Log in to see Polyclonal
0.00089556724 ABIN152025 IP PLA WB Rabbit C-Term Log in to see Polyclonal
0.00089556724 ABIN1585149 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.00089556724 ABIN1495153 IP Rabbit N-Term Log in to see Polyclonal
0.00089556724 ABIN1495152 IP Rabbit N-Term Log in to see Polyclonal
0.00089556724 ABIN223459 IP WB Rabbit IgG Log in to see Polyclonal
0.00089556724 ABIN242129 IP WB Rabbit Log in to see Polyclonal
0.00089556724 ABIN1931399 IP PLA WB Rabbit IgG C-Term Log in to see Polyclonal
0.00089556724 ABIN1931392 IP WB Rabbit IgG AA 1-100 Log in to see Polyclonal
0.00089556724 ABIN2353163 IP WB Rabbit IgG Log in to see Polyclonal
0.00089556724 ABIN4350925 IP WB Rabbit AA 2-543 Log in to see A9W4
0.00089556724 ABIN355818 IP WB Rabbit AA 1-100 Log in to see Polyclonal
0.00089556724 ABIN355819 IP WB Rabbit C-Term Log in to see Polyclonal
0.00089556724 ABIN1003088 IP WB Rabbit IgG Log in to see Polyclonal
0.00089556724 ABIN2976230 ICC IF IP IHC WB Rabbit IgG Log in to see Polyclonal
0.00089556724 ABIN2150708 IP WB Rabbit IgG Log in to see Polyclonal
0.00089556724 ABIN2150587 IP WB Rabbit IgG Log in to see Polyclonal
0.00089556724 ABIN1690168 IP Magnetic Particles Rabbit Log in to see Polyclonal

Top referenced anti-ROCK2 antibody for Immunoprecipitation

Similar anti-ROCK2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 19 Antibodies 19 Antibodies 45 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 5 Antibodies
Immunofluorescence (IF) 10 Antibodies 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies 34 Antibodies
Immunohistochemistry (IHC) 15 Antibodies 15 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies 21 Antibodies
Immunoprecipitation (IP) 8 Antibodies 10 Antibodies
Western Blotting (WB) 54 Antibodies 56 Antibodies

General

Antigen rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies
Epitope Center
(15), (14), (13), (13), (6), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(158), (90), (88), (4), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(137), (21), (1)
Clonality
Polyclonal
Conjugate This ROCK2 antibody is un-conjugated
(5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(118), (45), (38), (34), (30), (23), (19), (11), (3), (1), (1)
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Product Details anti-ROCK2 Antibody

Target Details ROCK2 Application Details Handling Images
Characteristics Rabbit Polyclonal antibody to ROCK2 (Rho-associated, coiled-coil containing protein kinase 2)
ROCK2 antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human ROCK2. The exact sequence is proprietary.
Isotype IgG

Target Details ROCK2

Product Details anti-ROCK2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ROCK2 (ROCK2 Antibody Abstract)
Background The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Cellular Localization: Cytoplasm (By similarity) , Cell membrane, Peripheral membrane protein (By similarity)
Molecular Weight 161 kDa
Gene ID 9475
Research Area Cancer, Apoptosis/Necrosis
Pathways Microtubule Dynamics, WNT Signaling

Application Details

Product Details anti-ROCK2 Antibody Target Details ROCK2 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , HeLa , HepG2 , Rat2 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , A549 , H1299 , HCT116

Restrictions For Research Use only

Handling

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Images back to top
Format Liquid
Concentration 0.67 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-ROCK2 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 2) (Center) (ABIN2855440) WB Image ROCK2 antibody detects ROCK2 protein by western blot analysis. A. 30 μg A549...
Immunohistochemistry (IHC) image for anti-ROCK2 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 2) (Center) (ABIN2855440) IHC-P Image ROCK2 antibody detects ROCK2 protein at cytosol on mouse kidney by immuno...
Immunofluorescence (IF) image for anti-ROCK2 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 2) (Center) (ABIN2855440) ICC/IF Image ROCK2 antibody detects ROCK2 protein at cytoplasm by immunofluorescent a...
Western Blotting (WB) image for anti-ROCK2 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 2) (Center) (ABIN2855440) WB Image ROCK2 antibody detects ROCK2 protein by western blot analysis. A. 30 μg Rat2...
Western Blotting (WB) image for anti-ROCK2 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 2) (Center) (ABIN2855440) WB Image ROCK2 antibody detects ROCK2 protein by western blot analysis. A. 30 μg Neur...
Immunoprecipitation (IP) image for anti-ROCK2 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 2) (Center) (ABIN2855440) IP Image Immunoprecipitation of ROCK2 protein from HeLa whole cell extracts using 5 μ...
Western Blotting (WB) image for anti-ROCK2 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 2) (Center) (ABIN2855440) WB Image ROCK2 antibody detects ROCK2 protein by western blot analysis. Various whole...