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anti-Human ROCK2 antibody for Proximity Ligation Assay

Recommended ROCK2 Antibody (supplied by: Log in to see )

Antigen
rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies
  • rocka-a
  • xROK
  • rock2
  • rok2
  • ROCK2
  • B230113H15Rik
  • ROKalpha
  • Rho-kinase
  • Rock-II
  • Rock2m
  • mKIAA0619
  • ROCK-II
  • ROK
  • Rho-associated, coiled-coil containing protein kinase 2
  • rho-associated, coiled-coil containing protein kinase 2a
  • Rho-associated coiled-coil containing protein kinase 2
  • Rho-associated coiled-coil containing protein kinase 2-like
  • rock2
  • rock2a
  • ROCK2
  • Rock2
  • LOC100346837
Epitope
C-Term
17
15
14
13
6
6
6
5
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human
168
99
97
4
3
2
2
1
1
1
1
Host
Rabbit
147
21
1
Clonality
Polyclonal
Conjugate
This ROCK2 antibody is un-conjugated
5
4
4
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
Application
Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
121
44
40
40
31
24
23
11
3
1
Supplier
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Available images

Catalog No. ABIN152025
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1931399 IP PLA WB Rabbit IgG C-Term Log in to see Polyclonal

Top referenced anti-ROCK2 antibody for Proximity Ligation Assay

Similar anti-ROCK2 Antibodies

Application / Reactivity Human
ELISA 44 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Immunocytochemistry (ICC) 11 Antibodies
Immunofluorescence (IF) 24 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies
Immunohistochemistry (IHC) 31 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 40 Antibodies
Immunoprecipitation (IP) 24 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 121 Antibodies

General

Antigen rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies
Epitope C-Term
(17), (15), (14), (13), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(168), (99), (97), (4), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(147), (21), (1)
Clonality
Polyclonal
Conjugate This ROCK2 antibody is un-conjugated
(5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
(121), (44), (40), (40), (31), (24), (23), (11), (3), (1)
Pubmed 1 reference available
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Product Details anti-ROCK2 Antibody

Target Details ROCK2 Application Details Handling References for anti-ROCK2 Antibody (ABIN152025) Images
Specificity This antibody is specific for human Rho Kinase alpha protein.
Purification Immunogen affinity purified
Immunogen The immunogen recognized by this antibody maps to a region between residues 1320 and the C-terminus (residues 1388) of human ROCK2 using the number given in entry NP_004841.1 (GeneID 9475)

Target Details ROCK2

Product Details anti-ROCK2 Antibody Application Details Handling References for anti-ROCK2 Antibody (ABIN152025) Images back to top
Antigen
Alternative Name ROCK2 (ROCK2 Antibody Abstract)
Background Gene Symbol: ROCK2
Gene ID 9475
UniProt O75116
Research Area Cancer, Apoptosis/Necrosis
Pathways Microtubule Dynamics, WNT Signaling

Application Details

Product Details anti-ROCK2 Antibody Target Details ROCK2 Handling References for anti-ROCK2 Antibody (ABIN152025) Images back to top
Application Notes Western Blot 1:10000-1:25000, Immunoprecipitation 2-5 μg/mg lysate, Proximity Ligation Assay 1:1000-1:10000Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:10,000 - 1:25,000 Immunoprecipitation: 2 to 5 μg/mg of lysate Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details References for anti-ROCK2 Antibody (ABIN152025) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-ROCK2 Antibody (ABIN152025)

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Handling Images back to top
Product cited in:

Shum, Da Silva, Belleannée, McKee, Brown, Breton: "Regulation of V-ATPase recycling via a RhoA- and ROCKII-dependent pathway in epididymal clear cells." in: American journal of physiology. Cell physiology, Vol. 301, Issue 1, pp. C31-43, 2011

Images

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Handling References for anti-ROCK2 Antibody (ABIN152025) back to top
Supplier Images
Western Blotting (WB) image for anti-ROCK2 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 2) (C-Term) (ABIN152025) Detection of Human Rho Kinase Alpha by Western Blot and Immunoprecipitation. Samples:...
Proximity Ligation Assay (PLA) image for anti-ROCK2 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 2) (C-Term) (ABIN152025) Proximity Ligation Assay: ROCK2 Antibody [ABIN152025] - Secondary-conjugate Duolink I...