anti-Human ROCK2 antibody for Western Blotting

Recommended ROCK2 Antibody (supplied by: Log in to see )

Antigen
rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies
  • rocka-a
  • xROK
  • rock2
  • rok2
  • ROCK2
  • B230113H15Rik
  • ROKalpha
  • Rho-kinase
  • Rock-II
  • Rock2m
  • mKIAA0619
  • ROCK-II
  • ROK
  • Rho-associated, coiled-coil containing protein kinase 2
  • rho-associated, coiled-coil containing protein kinase 2a
  • Rho-associated coiled-coil containing protein kinase 2
  • Rho-associated coiled-coil containing protein kinase 2-like
  • rock2
  • rock2a
  • ROCK2
  • Rock2
  • LOC100346837
Epitope
AA 652-923
16
15
15
12
6
6
5
5
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
173
119
113
9
5
5
3
3
2
2
1
1
1
1
1
1
Host
Rabbit
152
21
1
Clonality
Polyclonal
Conjugate
This ROCK2 antibody is un-conjugated
5
5
4
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
129
46
40
37
36
28
25
11
3
3
2
Options
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Catalog No. ABIN3043921
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2206945 ABIN968070 IF IP IHC WB Mouse IgG1 AA 567-718 Log in to see 21-ROCK 5
3.2206945 ABIN968071 IF IP IHC WB Mouse IgG1 AA 567-718 Log in to see 21-ROCK 5
3.2206945 ABIN152024 IP WB Rabbit AA 1-100 Log in to see Polyclonal 2
3.2206945 ABIN152025 IP PLA WB Rabbit C-Term Log in to see Polyclonal 1
3.2206945 ABIN3044353 IHC (p) WB Rabbit IgG AA 35-51, N-Term Log in to see Polyclonal 2
3.2206945 ABIN522961 IF IHC (p) ELISA WB Mouse IgG1 kappa AA 1279-1388, partial Log in to see 1E12 1
3.2206945 ABIN4350923 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
3.2206945 ABIN393426 IF WB Mouse IgG1 kappa AA 1279-1389 Log in to see 1E12
3.2206945 ABIN3186830 ELISA WB Rabbit IgG Tyr466 Log in to see Polyclonal
3.2206945 ABIN3187730 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
3.2206945 ABIN2705194 WB Rabbit C-Term Log in to see Polyclonal
3.2206945 ABIN614694 EIA IF IHC (p) WB Mouse IgG1 AA 1279-1389 Log in to see 1-00E-012
3.2206945 ABIN615037 IHC (p) WB Rabbit IgG Log in to see Polyclonal
3.2206945 ABIN2855440 ICC IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
3.2206945 ABIN393555 IF WB Mouse IgG2b kappa AA 1279-1389 Log in to see 2A4
3.2206945 ABIN4350921 ELISA ICC IF IHC WB Rabbit Center Log in to see Polyclonal
3.2206945 ABIN4350925 IP WB Rabbit AA 2-543 Log in to see A9W4
3.2206945 ABIN4350924 ICC IF IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
3.2206945 ABIN1876899 IF IHC IP WB Rabbit IgG Log in to see Polyclonal
3.2206945 ABIN233829 ELISA WB Rabbit IgG pTyr256 Log in to see Polyclonal

General

Antigen rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies
Epitope AA 652-923
(16), (15), (15), (12), (6), (6), (5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(173), (119), (113), (9), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(152), (21), (1)
Clonality
Polyclonal
Conjugate This ROCK2 antibody is un-conjugated
(5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(129), (46), (40), (37), (36), (28), (25), (11), (3), (3), (2)
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Product Details anti-ROCK2 Antibody

Target Details ROCK2 Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for Rho-associated protein kinase 2(ROCK2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Rho-associated protein kinase 2(ROCK2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: Rho-associated, coiled-coil containing protein kinase 2
Protein Name: Rho-associated protein kinase 2
Purification Immunogen affinity purified.
Immunogen E.coli-derived human ROCK2 recombinant protein (Position: E652-D923). Human ROCK2 shares 94.9% and 95.2% amino acid (aa) sequence identity with mouse and rat ROCK2, respectively.
Isotype IgG

Target Details ROCK2

Product Details anti-ROCK2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ROCK2 (ROCK2 Antibody Abstract)
Background Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. Serine/threonine kinase ROCK II/Rho kinase, which is an isozyme of ROCK I, is one of the targets for the small GTPase Rho. ROCK II regulates the formation of actin stress fibers and focal adhesions, cytokinesis, smooth muscle contraction, and the activation of c-fos serum response element. Sequencing analysis has shown that human ROCK II contains 1388 amino acid residues with a calculated molecular mass of approximately 161 kDa. Fluorescence in situ hybridization analysis showed that the human ROCK II gene is located on chromosome 2p24. Thumkeo et al. concluded that ROCK-II is essential in inhibiting blood coagulation and maintaining blood flow in the endothelium-free labyrinth layer and that loss of ROCK-II leads to thrombus formation, placental dysfunction, intrauterine growth retardation, and fetal death.

Synonyms: coiled-coil-containing protein kinase 2 antibody|KIAA0619 antibody|p164 ROCK 2 antibody|p164 ROCK-2 antibody|Rho associated coiled coil containing protein kinase 2 antibody|Rho associated protein kinase 2 antibody|Rho associated, coiled coil containing protein kinase II antibody|Rho kinase 2 antibody|Rho-associated antibody|Rho-associated protein kinase 2 antibody|ROCK 2 antibody|Rock II antibody|Rock2 antibody|ROCK2_HUMAN antibody|Rock2m antibody|ROK alpha antibody|ROKalpha antibody
Gene ID 9475
UniProt O75116
Research Area Cancer, Apoptosis/Necrosis
Pathways Microtubule Dynamics, WNT Signaling, Tube Formation

Application Details

Product Details anti-ROCK2 Antibody Target Details ROCK2 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for ROCK2 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (AA 652-923) antibody (ABIN3043921) Anti- ROCK2 Picoband antibody,IHC(P) IHC(P): Human Mammary Cancer Tissue
Western Blotting (WB) image for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (AA 652-923) antibody (ABIN3043921) anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (AA 652-923) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (AA 652-923) antibody (ABIN3043921) Anti- ROCK2 Picoband antibody,IHC(P) IHC(P): Mouse Cardiac Muscle Tissue
Immunohistochemistry (IHC) image for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (AA 652-923) antibody (ABIN3043921) Anti- ROCK2 Picoband antibody,IHC(P) IHC(P): Rat Brain Tissue