anti-Human ROCK2 antibody for Western Blotting

Recommended ROCK2 Antibody (supplied by: Log in to see )

Antigen
rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies
  • rocka-a
  • xROK
  • rock2
  • rok2
  • ROCK2
  • B230113H15Rik
  • ROKalpha
  • Rho-kinase
  • Rock-II
  • Rock2m
  • mKIAA0619
  • ROCK-II
  • ROK
  • Rho-associated, coiled-coil containing protein kinase 2
  • rho-associated, coiled-coil containing protein kinase 2a
  • Rho-associated coiled-coil containing protein kinase 2
  • Rho-associated coiled-coil containing protein kinase 2-like
  • rock2
  • rock2a
  • ROCK2
  • Rock2
  • LOC100346837
Epitope
C-Term
16
15
15
12
6
6
5
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
170
118
113
9
5
5
3
3
2
2
1
1
1
1
1
1
Host
Rabbit
149
21
1
Clonality
Polyclonal
Conjugate
This ROCK2 antibody is un-conjugated
5
5
4
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
Application
Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
126
45
40
38
35
27
25
11
3
3
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN152025
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3489225 ABIN152024 IP WB Rabbit AA 1-100 Log in to see Polyclonal 2
3.3489225 ABIN3043921 IHC (p) WB Rabbit IgG AA 652-923 Log in to see Polyclonal
3.3489225 ABIN968070 IF IP IHC WB Mouse IgG1 AA 567-718 Log in to see 21-ROCK 5
3.3489225 ABIN968071 IF IP IHC WB Mouse IgG1 AA 567-718 Log in to see 21-ROCK 5
3.3489225 ABIN3044353 IHC (p) WB Rabbit IgG AA 35-51, N-Term Log in to see Polyclonal 2
3.3489225 ABIN4350923 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
3.3489225 ABIN522961 IF IHC (p) ELISA WB Mouse IgG1 kappa AA 1279-1388, partial Log in to see 1E12 1
3.3489225 ABIN393426 IF WB Mouse IgG1 kappa AA 1279-1389 Log in to see 1E12
3.3489225 ABIN2705194 WB Rabbit C-Term Log in to see Polyclonal
3.3489225 ABIN614694 EIA IF IHC (p) WB Mouse IgG1 AA 1279-1389 Log in to see 1-00E-012
3.3489225 ABIN615037 IHC (p) WB Rabbit IgG Log in to see Polyclonal
3.3489225 ABIN3186830 ELISA WB Rabbit IgG Tyr466 Log in to see Polyclonal
3.3489225 ABIN3187730 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
3.3489225 ABIN2855440 ICC IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
3.3489225 ABIN4350921 ELISA ICC IF IHC WB Rabbit Center Log in to see Polyclonal
3.3489225 ABIN4350924 ICC IF IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
3.3489225 ABIN4350925 IP WB Rabbit AA 2-543 Log in to see A9W4
3.3489225 ABIN393555 IF WB Mouse IgG2b kappa AA 1279-1389 Log in to see 2A4
3.3489225 ABIN1876899 IF IHC IP WB Rabbit IgG Log in to see Polyclonal
3.3489225 ABIN233829 ELISA WB Rabbit IgG pTyr256 Log in to see Polyclonal

Top referenced anti-ROCK2 antibody for Western Blotting

Similar anti-ROCK2 Antibodies

Application / Reactivity Human
ELISA 45 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Immunocytochemistry (ICC) 11 Antibodies
Immunofluorescence (IF) 25 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies
Immunohistochemistry (IHC) 35 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 38 Antibodies
Immunoprecipitation (IP) 28 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 126 Antibodies

General

Antigen rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies
Epitope C-Term
(16), (15), (15), (12), (6), (6), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(170), (118), (113), (9), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(149), (21), (1)
Clonality
Polyclonal
Conjugate This ROCK2 antibody is un-conjugated
(5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Application Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
(126), (45), (40), (38), (35), (27), (25), (11), (3), (3), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-ROCK2 Antibody

Target Details ROCK2 Application Details Handling ProductDetails: References for anti-ROCK2 antibody (ABIN152025) Images
Specificity This antibody is specific for human Rho Kinase alpha protein.
Purification Immunogen affinity purified
Immunogen The immunogen recognized by this antibody maps to a region between residues 1320 and the C-terminus (residues 1388) of human ROCK2 using the number given in entry NP_004841.1 (GeneID 9475)

Target Details ROCK2

Product Details anti-ROCK2 Antibody Application Details Handling ProductDetails: References for anti-ROCK2 antibody (ABIN152025) Images back to top
Antigen
Alternative Name ROCK2 (ROCK2 Antibody Abstract)
Background Gene Symbol: ROCK2
Gene ID 9475
UniProt O75116
Research Area Cancer, Apoptosis/Necrosis
Pathways Microtubule Dynamics, WNT Signaling, Tube Formation

Application Details

Product Details anti-ROCK2 Antibody Target Details ROCK2 Handling ProductDetails: References for anti-ROCK2 antibody (ABIN152025) Images back to top
Application Notes Western Blot 1:10000-1:25000, Immunoprecipitation 2-5 μg/mg lysate, Proximity Ligation Assay 1:1000-1:10000Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:10,000 - 1:25,000 Immunoprecipitation: 2 to 5 μg/mg of lysate Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details ProductDetails: References for anti-ROCK2 antibody (ABIN152025) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

ProductDetails: References for anti-ROCK2 antibody (ABIN152025)

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Handling Images back to top
Product cited in:

Shum, Da Silva, Belleannée, McKee, Brown, Breton: "Regulation of V-ATPase recycling via a RhoA- and ROCKII-dependent pathway in epididymal clear cells." in: American journal of physiology. Cell physiology, Vol. 301, Issue 1, pp. C31-43, 2011

Images

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Handling ProductDetails: References for anti-ROCK2 antibody (ABIN152025) back to top
Supplier Images
Western Blotting (WB) image for anti-ROCK2 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 2) (C-Term) (ABIN152025) Detection of Human Rho Kinase Alpha by Western Blot and Immunoprecipitation. Samples:...
Proximity Ligation Assay (PLA) image for anti-ROCK2 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 2) (C-Term) (ABIN152025) Proximity Ligation Assay: ROCK2 Antibody [ABIN152025] - Secondary-conjugate Duolink I...