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anti-Human ROCK2 antibody for Western Blotting

Recommended ROCK2 Antibody (supplied by: Log in to see )

Antigen
rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies
  • B230113H15Rik
  • mKIAA0619
  • Rho-kinase
  • Rock-II
  • ROCK-II
  • rock2
  • ROCK2
  • Rock2m
  • rocka-a
  • ROK
  • rok2
  • ROKalpha
  • xROK
Epitope
Center
15
14
13
13
6
6
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
158
90
88
4
3
2
2
1
1
1
1
Host
Rabbit
137
21
1
Clonality
Polyclonal
Conjugate
This ROCK2 antibody is un-conjugated
5
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
118
45
39
34
30
24
20
12
2
1
1
Supplier
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Catalog No. ABIN783448
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13476625 ABIN968071 IF IP IHC WB Mouse IgG1 AA 567-718 Log in to see 21-ROCK 5
0.13476625 ABIN968070 IF IP IHC WB Mouse IgG1 AA 567-718 Log in to see 21-ROCK 5
0.13476625 ABIN522961 IF IHC (p) ELISA WB Mouse IgG1 kappa AA 1279-1388, partial Log in to see 1E12 1
0.13476625 ABIN3043921 IHC (p) WB Rabbit IgG AA 652-923 Log in to see Polyclonal
0.13476625 ABIN614694 EIA IF IHC (p) WB Mouse IgG1 AA 1279-1389 Log in to see 1-00E-012
0.13476625 ABIN615037 IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.13476625 ABIN2705194 WB Rabbit C-Term Log in to see Polyclonal
0.13476625 ABIN393426 IF WB Mouse IgG1 kappa AA 1279-1389 Log in to see 1E12
0.13476625 ABIN3044353 IHC (p) WB Rabbit IgG AA 35-51, N-Term Log in to see Polyclonal
0.13476625 ABIN393555 IF WB Mouse IgG2b kappa AA 1279-1389 Log in to see 2A4
0.13476625 ABIN2855440 ICC IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.13476625 ABIN2788813 IHC WB Rabbit Middle Region Log in to see Polyclonal
0.13476625 ABIN233829 ELISA WB Rabbit IgG pTyr256 Log in to see Polyclonal
0.13476625 ABIN1876899 IHC WB Rabbit IgG Log in to see Polyclonal
0.13476625 ABIN152024 IP WB Rabbit AA 1-100 Log in to see Polyclonal
0.13476625 ABIN152025 IP PLA WB Rabbit C-Term Log in to see Polyclonal
0.13476625 ABIN1585149 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.13476625 ABIN564096 IF ELISA WB Mouse IgG2b kappa AA 1279-1388, partial Log in to see 2A4
0.13476625 ABIN1031072 ELISA WB Rabbit Middle Region Log in to see Polyclonal
0.13476625 ABIN3186830 ELISA WB Rabbit IgG Tyr466 Log in to see Polyclonal

Top referenced anti-ROCK2 antibody for Western Blotting

Similar anti-ROCK2 Antibodies

Application / Reactivity Human Rat (Rattus) Mouse (Murine)
Western Blotting (WB) 120 Antibodies 54 Antibodies 56 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 22 Antibodies 8 Antibodies 10 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 39 Antibodies 21 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 30 Antibodies 15 Antibodies 15 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies 34 Antibodies 34 Antibodies
Immunofluorescence (IF) 24 Antibodies 10 Antibodies

General

Antigen rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies
Epitope Center
(15), (14), (13), (13), (6), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(158), (90), (88), (4), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(137), (21), (1)
Clonality
Polyclonal
Conjugate This ROCK2 antibody is un-conjugated
(5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(118), (45), (39), (34), (30), (24), (20), (12), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-ROCK2 Antibody

Target Details ROCK2 Application Details Handling References for anti-ROCK2 Antibody (ABIN783448) Images
Specificity This antibody detects ROCK2 at Center. Three isoforms of ROCK2 are known to exist, this antibody recognizes all three isoforms. ROCK2 antibody is predicted to not cross-react with other ROCK protein family members.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Affinity chromatography purified via peptide column
Immunogen 15 amino acid peptide near the center of human ROC

Target Details ROCK2

Product Details anti-ROCK2 Antibody Application Details Handling References for anti-ROCK2 Antibody (ABIN783448) Images back to top
Antigen
Alternative Name ROCK2 (ROCK2 Antibody Abstract)
Background ROCK2 is a member of the AGC serine/threonine protein kinase family that regulates the assembly of the actin cytoskeleton. ROCK2 contains a protein kinase domain, a coiled-coil region and a zinc finger region and appears to be present as a dimer. ROCK2, like its isozyme ROCK1, is a downstream target of the small G-protein Rho and plays a role in smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis, and mediates the cellular morphological changes during apoptosis.Synonyms: KIAA0619, ROCK-2, Rho kinase 2, Rho-associated, Rho-associated protein kinase 2, coiled-coil-containing protein kinase 2, p164ROCK-2
Gene ID 9475
NCBI Accession NP_004841
UniProt O75116
Research Area Cancer, Apoptosis/Necrosis
Pathways Microtubule Dynamics, WNT Signaling

Application Details

Product Details anti-ROCK2 Antibody Target Details ROCK2 Handling References for anti-ROCK2 Antibody (ABIN783448) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details References for anti-ROCK2 Antibody (ABIN783448) Images back to top
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer.

References for anti-ROCK2 Antibody (ABIN783448)

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Handling Images back to top
Background publications

Takahashi, Tuiki, Saya, Kaibuchi: "Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24." in: Genomics, Vol. 55, Issue 2, pp. 235-7, 1999

Leung, Chen, Manser, Lim: "The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton." in: Molecular and cellular biology, Vol. 16, Issue 10, pp. 5313-27, 1996

Images

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Handling References for anti-ROCK2 Antibody (ABIN783448) back to top
Supplier Images
Western Blotting (WB) image for anti-ROCK2 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 2) (Center) (ABIN783448) Western blot analysis of ROCK2 in mouse brain tissue lysate with ROCK2 antibody at (A...