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anti-Xenopus laevis ROCK2 antibody for Enzyme Immunoassay

Recommended ROCK2 Antibody (supplied by: Log in to see )

Antigen
rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies
  • B230113H15Rik
  • mKIAA0619
  • Rho-kinase
  • Rock-II
  • ROCK-II
  • rock2
  • ROCK2
  • Rock2m
  • rocka-a
  • ROK
  • rok2
  • ROKalpha
  • xROK
Epitope
pTyr256
15
14
13
13
6
6
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Chicken, Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
153
85
83
2
2
2
2
1
Host
Rabbit
132
21
1
Clonality
Polyclonal
Conjugate
This ROCK2 antibody is un-conjugated
5
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
113
43
36
34
26
21
19
9
2
1
1
Supplier
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Catalog No. ABIN401393
$ 500.50
Plus shipping costs $45.00

Top referenced anti-ROCK2 antibody for Enzyme Immunoassay

Similar anti-ROCK2 Antibodies

Application / Reactivity Human Rat (Rattus) Mouse (Murine) Cow (Bovine) Dog (Canine) Chicken Fruit Fly (Drosophila melanogaster) Xenopus laevis
Western Blotting (WB) 115 Antibodies 49 Antibodies 51 Antibodies 2 Antibodies 3 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 21 Antibodies 7 Antibodies 9 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies 18 Antibodies 19 Antibodies
Immunohistochemistry (IHC) 26 Antibodies 2 Antibodies 11 Antibodies

General

Antigen rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies
Epitope pTyr256
(15), (14), (13), (13), (6), (6), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Chicken, Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(153), (85), (83), (2), (2), (2), (2), (1)
Host Rabbit
(132), (21), (1)
Clonality
Polyclonal
Conjugate This ROCK2 antibody is un-conjugated
(5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(113), (43), (36), (34), (26), (21), (19), (9), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-ROCK2 Antibody

Target Details ROCK2 Application Details Handling References for anti-ROCK2 Antibody (ABIN401393) Images
Specificity This phospho-specific polyclonal antibody reacts with Human ROCK-2 pTyr256 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Expect a band approximately 164 kDa in size corresponding to phosphorylated ROCK-2
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Chicken, Canine, Bovine, Xenopus, Drosophila.
Species reactivity (tested):Human.
Purification Affinity Chromatography.
Immunogen Synthetic peptide corresponding to residues surrounding Y256 of human ROCK-2
Isotype IgG

Target Details ROCK2

Product Details anti-ROCK2 Antibody Application Details Handling References for anti-ROCK2 Antibody (ABIN401393) Images back to top
Antigen
Alternative Name ROCK2 (ROCK2 Antibody Abstract)
Background Progression of tumors to invasive and metastatic forms requires that tumor cells undergo dramatic morphological changes, a process regulated by Rho GTPases. Increased levels of RhoA or Rh °C, as well as the Rho effector proteins ROCK-1 or ROCK-2, were observed in various human carcinomas. In vivo studies have also shown that ROCK inhibition reduced the invasiveness of several tumor cell lines. It is suggested that Rho and ROCK signaling contribute to the morphological changes and metastatic behavior of some tumor cells. Thus, elevated Rho and ROCK expression is associated with tumorigenesis, particularly during the progression to invasive and metastatic phenotypes. Phosphorylation of certain residues in ROCK-2 may be critical for its kinase activity.Synonyms: KIAA0619, ROCK-2, Rho kinase 2, Rho-associated, Rho-associated protein kinase 2, coiled-coil-containing protein kinase 2, p164ROCK-2
Gene ID 9475
NCBI Accession NP_004841
UniProt O75116
Research Area Cancer, Apoptosis/Necrosis
Pathways Microtubule Dynamics, WNT Signaling

Application Details

Product Details anti-ROCK2 Antibody Target Details ROCK2 Handling References for anti-ROCK2 Antibody (ABIN401393) Images back to top
Application Notes ELISA: 1/2,000-1/10,000. Western Blot: 1/200-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details References for anti-ROCK2 Antibody (ABIN401393) Images back to top
Concentration 1.1 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-28 °C for longer.

References for anti-ROCK2 Antibody (ABIN401393)

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Handling Images back to top
Background publications

Fritz, Kaina: "Rho GTPases: promising cellular targets for novel anticancer drugs." in: Current cancer drug targets, Vol. 6, Issue 1, pp. 1-14, 2006

Schwartz: "Rho signalling at a glance." in: Journal of cell science, Vol. 117, Issue Pt 23, pp. 5457-8, 2004

Takahashi, Tuiki, Saya, Kaibuchi: "Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24." in: Genomics, Vol. 55, Issue 2, pp. 235-7, 1999

Images

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Handling References for anti-ROCK2 Antibody (ABIN401393) back to top
Supplier Images
 image for anti-ROCK2 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 2) (pTyr256) (ABIN401393) anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (pTyr256) antibody