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anti-Human TUBB antibody for Immunohistochemistry (Frozen Sections)

Recommended TUBB Antibody (supplied by: Log in to see )

Antigen
Tubulin, beta (TUBB) Antibodies
  • 1t
  • 2t
  • 3t
  • 4t
  • 143391_i_at
  • AA408537
  • AI325297
  • AI596182
  • B1t
  • B2t
  • B3t
  • B4t
  • B130022C14Rik
  • beta
  • beta(2)Tu
  • beta(2)Tub
  • beta-5
  • beta-tub
  • beta-Tub
  • beta-Tub6D
  • beta-Tub56D
  • beta-Tub60D
  • beta-Tub85D
  • beta-tub85D
  • beta-Tub97EF
  • beta-tubulin56D
  • beta1
  • beta1-tub
  • beta1-Tubulin
  • beta1t
  • beta1tub
  • beta1Tub
  • BETA2
  • beta2
  • beta2-tub
  • beta2t
  • beta2tub
  • beta3
  • beta3 TU
  • beta3-Tub
  • beta3-tubulin
  • beta3t
  • beta3TUB
  • beta3Tub
  • beta4
  • beta4t
  • beta56D
  • BETA 56D
  • beta60C
  • BETA 60D
  • BETA 85D
  • beta85D
  • beta97F
  • BETA 98B
  • betaTub
  • betatub(56D)
  • betaTub1
  • betaTub2
  • betaTub3
  • betaTub4
  • betaTub60C
  • betatub60D
  • betaTub98BC
  • betaTub98C
  • beta[[1]]-tubulin
  • beta[[1]] tubulin
  • beta[[2]]-tubulin
  • beta[[3]]-Tub
  • beta[[3]]-tubulin
  • beta[[3]] tubulin
  • bmtub1
  • bmtub2
  • bmtub3
  • bmtub4
  • CG3401
  • CG4869
  • CG9277
  • CG9359
  • D.m.BETA-60D
  • D.m.BETA-85D
  • Dmbeta1
  • Dmbeta2
  • Dmbeta3
  • Dmbeta4
  • Dmel\\CG3401
  • Dmel\\CG4869
  • Dmel\\CG9277
  • Dmel\\CG9359
  • DTB1
  • DTB2
  • DTB3
  • DTB4
  • LOC100101153
  • M(beta)4
  • M(beta)5
  • M40
  • m40
  • ms(3)KK[D]
  • OK/SW-cl.56
  • ok/sw-cl.56
  • p50
  • p50/tubulin
  • p53
  • p53/tubulin
  • T
  • Tub
  • Tub1
  • Tub2
  • Tub3
  • Tub4
  • tub5
  • Tub60D
  • Tubb
  • TUBB
  • TUBB1
  • tubb1
  • TUBB2A
  • tubb2a
  • TUBB2B
  • tubB3
  • tubb4
  • Tubb4
  • tubb5
  • TUBB5
  • tubulin
  • Tubulin
  • XLOT
Reactivity
Human, Mouse (Murine), Plant, Pig (Porcine)
314
193
192
56
48
36
32
24
17
17
15
15
9
8
7
6
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Host
Mouse
219
146
2
1
Clonality
Monoclonal
Conjugate
This TUBB antibody is un-conjugated
13
8
6
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
315
137
110
109
94
82
23
23
19
9
7
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN238519
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000883884 ABIN192340 ICC IHC (fro) WB Mouse IgM N-Term Log in to see TU-13 3
0.000883884 ABIN285994 IHC (fro) IHC (p) IP WB Rabbit AA 416-430 Log in to see Polyclonal
0.000883884 ABIN1860883 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 170-419 Log in to see Polyclonal
0.000883884 ABIN2477001 IHC (fro) ELISA WB Mouse IgM Log in to see TU-13 3
0.000883884 ABIN3199337 ICC IHC (fro) WB Mouse IgM Log in to see TU-13

Top referenced anti-TUBB antibody for Immunohistochemistry (Frozen Sections)

Similar anti-TUBB Antibodies

Application / Reactivity Plant Pig (Porcine)
ELISA 1 Antibodies 11 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 35 Antibodies
Immunofluorescence (IF) 3 Antibodies 23 Antibodies
Immunohistochemistry (IHC) 2 Antibodies 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies 8 Antibodies
Western Blotting (WB) 6 Antibodies 56 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies
Immunoprecipitation (IP) 2 Antibodies

General

Antigen Tubulin, beta (TUBB) Antibodies
Reactivity Human, Mouse (Murine), Plant, Pig (Porcine)
(314), (193), (192), (56), (48), (36), (32), (24), (17), (17), (15), (15), (9), (8), (7), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(219), (146), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TUBB antibody is un-conjugated
(13), (8), (6), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
(315), (137), (110), (109), (94), (82), (23), (23), (19), (9), (7), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-TUBB Antibody

Target Details TUBB Application Details Handling References for anti-TUBB Antibody (ABIN238519) Images
Specificity The antibody TU-13 recognizes an epitope on N-terminal structural domain of beta-tubulin in various species.
Characteristics Synonyms: Tubulin beta chain, Tubulin beta-5 chain
Purification Precipitation Methods and Size-Exclusion Chromatography.
Immunogen beta-tubulin from porcine brain.
Clone TU-13
Isotype IgM

Target Details TUBB

Product Details anti-TUBB Antibody Application Details Handling References for anti-TUBB Antibody (ABIN238519) Images back to top
Antigen
Alternative Name TUBB / TUBB5 (TUBB Antibody Abstract)
Background The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening - this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.Synonyms: Tubulin beta chain, Tubulin beta-5 chain
Gene ID 203068
UniProt P07437
Pathways Microtubule Dynamics

Application Details

Product Details anti-TUBB Antibody Target Details TUBB Handling References for anti-TUBB Antibody (ABIN238519) Images back to top
Application Notes Western Blotting (Reducing Conditions): 1 μg/mL, Sonication of MCF7 cells isrecommended. Positive Control: RAJI human cell line, RAMOS human cell line, THP-1 human cell line, HeLa
Restrictions For Research Use only

Handling

Product Details anti-TUBB Antibody Target Details TUBB Application Details References for anti-TUBB Antibody (ABIN238519) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 with 15 mM Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch
Expiry Date 12 months

References for anti-TUBB Antibody (ABIN238519)

Product Details anti-TUBB Antibody Target Details TUBB Application Details Handling Images back to top
Background publications

Linhartová, Dráberová, Viklický, Dráber: "Distribution of non-class-III beta-tubulin isoforms in neuronal and non-neuronal cells." in: FEBS letters, Vol. 320, Issue 1, pp. 79-82, 1993

Dráber, Vater, Böhm, Kuklova, Unger: "Inhibition of microtubule assembly in vitro by anti-tubulin monoclonal antibodies." in: FEBS letters, Vol. 262, Issue 2, pp. 209-11, 1990

Dráber, Lagunowich, Dráberová, Viklický, Damjanov: "Heterogeneity of tubulin epitopes in mouse fetal tissues." in: Histochemistry, Vol. 89, Issue 5, pp. 485-92, 1988