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anti-Mouse (Murine) TUBB antibody for BioImaging

Recommended TUBB Antibody (supplied by: Log in to see )

Antigen
Tubulin, beta (TUBB) Antibodies
  • 1t
  • 2t
  • 3t
  • 4t
  • 143391_i_at
  • AA408537
  • AI325297
  • AI596182
  • B1t
  • B2t
  • B3t
  • B4t
  • B130022C14Rik
  • beta
  • beta(2)Tu
  • beta(2)Tub
  • beta-5
  • beta-tub
  • beta-Tub
  • beta-Tub6D
  • beta-Tub56D
  • beta-Tub60D
  • beta-Tub85D
  • beta-tub85D
  • beta-Tub97EF
  • beta-tubulin56D
  • beta1
  • beta1-tub
  • beta1-Tubulin
  • beta1t
  • beta1tub
  • beta1Tub
  • BETA2
  • beta2
  • beta2-tub
  • beta2t
  • beta2tub
  • beta3
  • beta3 TU
  • beta3-Tub
  • beta3-tubulin
  • beta3t
  • beta3TUB
  • beta3Tub
  • beta4
  • beta4t
  • beta56D
  • BETA 56D
  • beta60C
  • BETA 60D
  • BETA 85D
  • beta85D
  • beta97F
  • BETA 98B
  • betaTub
  • betatub(56D)
  • betaTub1
  • betaTub2
  • betaTub3
  • betaTub4
  • betaTub60C
  • betatub60D
  • betaTub98BC
  • betaTub98C
  • beta[[1]]-tubulin
  • beta[[1]] tubulin
  • beta[[2]]-tubulin
  • beta[[3]]-Tub
  • beta[[3]]-tubulin
  • beta[[3]] tubulin
  • bmtub1
  • bmtub2
  • bmtub3
  • bmtub4
  • CG3401
  • CG4869
  • CG9277
  • CG9359
  • D.m.BETA-60D
  • D.m.BETA-85D
  • Dmbeta1
  • Dmbeta2
  • Dmbeta3
  • Dmbeta4
  • Dmel\\CG3401
  • Dmel\\CG4869
  • Dmel\\CG9277
  • Dmel\\CG9359
  • DTB1
  • DTB2
  • DTB3
  • DTB4
  • LOC100101153
  • M(beta)4
  • M(beta)5
  • M40
  • m40
  • ms(3)KK[D]
  • OK/SW-cl.56
  • ok/sw-cl.56
  • p50
  • p50/tubulin
  • p53
  • p53/tubulin
  • T
  • Tub
  • Tub1
  • Tub2
  • Tub3
  • Tub4
  • tub5
  • Tub60D
  • Tubb
  • TUBB
  • TUBB1
  • tubb1
  • TUBB2A
  • tubb2a
  • TUBB2B
  • tubB3
  • tubb4
  • Tubb4
  • tubb5
  • TUBB5
  • tubulin
  • Tubulin
  • XLOT
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
310
189
187
54
44
31
31
20
17
14
14
12
9
8
7
6
5
5
5
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Host
Mouse
218
143
2
1
Clonality
Monoclonal
Conjugate
This TUBB antibody is un-conjugated
12
7
6
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
Application
BioImaging (BI), Immunohistochemistry (IHC), Western Blotting (WB)
311
136
109
107
92
81
23
23
18
10
7
2
2
1
1
1
1
1
1
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Catalog No. ABIN967495
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Top referenced anti-TUBB antibody for BioImaging

Similar anti-TUBB Antibodies

Application / Reactivity Mouse (Murine) Human Rat (Rattus) Cow (Bovine)
Western Blotting (WB) 168 Antibodies 273 Antibodies 159 Antibodies 31 Antibodies
Immunostaining (ISt) 1 Antibodies 1 Antibodies 1 Antibodies
Immunoprecipitation (IP) 6 Antibodies 14 Antibodies 14 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 43 Antibodies 73 Antibodies 40 Antibodies 4 Antibodies
Immunohistochemistry (IHC) 48 Antibodies

General

Antigen Tubulin, beta (TUBB) Antibodies
Reactivity Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(310), (189), (187), (54), (44), (31), (31), (20), (17), (14), (14), (12), (9), (8), (7), (6), (5), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(218), (143), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TUBB antibody is un-conjugated
(12), (7), (6), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application BioImaging (BI), Immunohistochemistry (IHC), Western Blotting (WB)
(311), (136), (109), (107), (92), (81), (23), (23), (18), (10), (7), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-TUBB Antibody

Target Details TUBB Application Details Handling References for anti-TUBB Antibody (ABIN967495) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
3. Triton is a trademark of the Dow Chemical Company.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Clone 5H1
Isotype IgM, kappa

Target Details TUBB

Product Details anti-TUBB Antibody Application Details Handling References for anti-TUBB Antibody (ABIN967495) Images back to top
Antigen
Alternative Name beta-Tubulin (TUBB Antibody Abstract)
Background Tubulin is a highly conserved protein with a molecular weight of ~50 kD. The self-assembly of tubulin leads to microtubules, hollow cylinders that are one of the major components of the eukaryotic cytoskeleton. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. There are two main classes of tubulin isoforms, alpha- and beta-tubulin, which are usually products of separate genes. Microtubules are made from protofilaments, strings of alternating alpha- and beta-tubulin spaced 4 nm apart and pointing in the same direction. Tubulin can be posttranslationally modified in several ways, including phosphorylation, acetylation, glutamylation, and detyrosination. For example, microtubules that turn over slowly tend to be acetylated and detyrosinated.
The 5H1 monoclonal antibody reacts with beta-tubulin. It does not cross-react with alpha-tubulin.
Molecular Weight 50 kDa
Pathways Microtubule Dynamics

Application Details

Product Details anti-TUBB Antibody Target Details TUBB Handling References for anti-TUBB Antibody (ABIN967495) Images back to top
Application Notes Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.
Comment

Related Products: ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-TUBB Antibody Target Details TUBB Application Details References for anti-TUBB Antibody (ABIN967495) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-TUBB Antibody (ABIN967495)

Product Details anti-TUBB Antibody Target Details TUBB Application Details Handling Images back to top
Product cited in:

Cho, Johnson: "Primed phosphorylation of tau at Thr231 by glycogen synthase kinase 3beta (GSK3beta) plays a critical role in regulating tau's ability to bind and stabilize microtubules." in: Journal of neurochemistry, Vol. 88, Issue 2, pp. 349-58, 2003

Helfand, Mikami, Vallee, Goldman: "A requirement for cytoplasmic dynein and dynactin in intermediate filament network assembly and organization." in: The Journal of cell biology, Vol. 157, Issue 5, pp. 795-806, 2002

Prahlad, Yoon, Moir, Vale, Goldman: "Rapid movements of vimentin on microtubule tracks: kinesin-dependent assembly of intermediate filament networks." in: The Journal of cell biology, Vol. 143, Issue 1, pp. 159-70, 1998

Brown, Wood, Cleveland: "The kinesin-like protein CENP-E is kinetochore-associated throughout poleward chromosome segregation during anaphase-A." in: Journal of cell science, Vol. 109 ( Pt 5), Issue 4, pp. 961-9, 1996

Wang, Loomis, Zinkowski, Binder: "A novel tau transcript in cultured human neuroblastoma cells expressing nuclear tau." in: The Journal of cell biology, Vol. 121, Issue 2, pp. 257-67, 1993

Background publications

Zeng, Tang, Xie, Liu, Lu, Chai, Wang, Yao, Qian: "Radioimmunotherapy for unresectable hepatocellular carcinoma using 131I-Hepama-1 mAb: preliminary results." in: Journal of cancer research and clinical oncology, Vol. 119, Issue 5, pp. 257-9, 1993

Images

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Supplier Images
Immunofluorescence (IF) image for anti-TUBB antibody (Tubulin, beta) (ABIN967495) Immunofluorescent staining of U-87 MG (ATCC HTB-14) cells. Cells were seeded in a 96 ...
 image for anti-TUBB antibody (Tubulin, beta) (ABIN967495) anti-Tubulin, beta (TUBB) antibody (Image 2)