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anti-Xenopus laevis TUBB antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended TUBB Antibody (supplied by: Log in to see )

Antigen
Tubulin, beta (TUBB) Antibodies
  • 1t
  • 2t
  • 3t
  • 4t
  • 143391_i_at
  • AA408537
  • AI325297
  • AI596182
  • B1t
  • B2t
  • B3t
  • B4t
  • B130022C14Rik
  • beta
  • beta(2)Tu
  • beta(2)Tub
  • beta-5
  • beta-tub
  • beta-Tub
  • beta-Tub6D
  • beta-Tub56D
  • beta-Tub60D
  • beta-Tub85D
  • beta-tub85D
  • beta-Tub97EF
  • beta-tubulin56D
  • beta1
  • beta1-tub
  • beta1-Tubulin
  • beta1t
  • beta1tub
  • beta1Tub
  • BETA2
  • beta2
  • beta2-tub
  • beta2t
  • beta2tub
  • beta3
  • beta3 TU
  • beta3-Tub
  • beta3-tubulin
  • beta3t
  • beta3TUB
  • beta3Tub
  • beta4
  • beta4t
  • beta56D
  • BETA 56D
  • beta60C
  • BETA 60D
  • BETA 85D
  • beta85D
  • beta97F
  • BETA 98B
  • betaTub
  • betatub(56D)
  • betaTub1
  • betaTub2
  • betaTub3
  • betaTub4
  • betaTub60C
  • betatub60D
  • betaTub98BC
  • betaTub98C
  • beta[[1]]-tubulin
  • beta[[1]] tubulin
  • beta[[2]]-tubulin
  • beta[[3]]-Tub
  • beta[[3]]-tubulin
  • beta[[3]] tubulin
  • bmtub1
  • bmtub2
  • bmtub3
  • bmtub4
  • CG3401
  • CG4869
  • CG9277
  • CG9359
  • D.m.BETA-60D
  • D.m.BETA-85D
  • Dmbeta1
  • Dmbeta2
  • Dmbeta3
  • Dmbeta4
  • Dmel\\CG3401
  • Dmel\\CG4869
  • Dmel\\CG9277
  • Dmel\\CG9359
  • DTB1
  • DTB2
  • DTB3
  • DTB4
  • LOC100101153
  • M(beta)4
  • M(beta)5
  • M40
  • m40
  • ms(3)KK[D]
  • OK/SW-cl.56
  • ok/sw-cl.56
  • p50
  • p50/tubulin
  • p53
  • p53/tubulin
  • T
  • Tub
  • Tub1
  • Tub2
  • Tub3
  • Tub4
  • tub5
  • Tub60D
  • Tubb
  • TUBB
  • TUBB1
  • tubb1
  • TUBB2A
  • tubb2a
  • TUBB2B
  • tubB3
  • tubb4
  • Tubb4
  • tubb5
  • TUBB5
  • tubulin
  • Tubulin
  • XLOT
Epitope
N-Term, AA 1-100
56
28
13
13
11
9
6
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Chinese Hamster, Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
314
193
191
56
47
35
32
23
17
16
15
14
9
8
7
6
5
5
5
4
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Host
Rabbit
220
145
2
1
Clonality
Polyclonal
Conjugate
This TUBB antibody is un-conjugated
13
8
6
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
315
137
110
108
93
81
23
23
19
10
7
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN269949
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008982679 ABIN1724358 IF IHC (p) WB Mouse IgG3 Log in to see
0.0008982679 ABIN1732316 IHC (p) WB Rabbit IgG AA 417-435 Log in to see Polyclonal
0.0008982679 ABIN4284181 IHC IHC (p) SimWes WB Rabbit Log in to see Polyclonal

Top referenced anti-TUBB antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-TUBB Antibodies

Application / Reactivity Chicken Cow (Bovine) Human Mouse (Murine) Primate Rat (Rattus) Xenopus laevis Pig (Porcine) Zebrafish (Danio rerio) Chinese Hamster
Simple Western (SimWes) 2 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 56 Antibodies 168 Antibodies 273 Antibodies 159 Antibodies 50 Antibodies 35 Antibodies 16 Antibodies 18 Antibodies 4 Antibodies 24 Antibodies
Immunostaining (ISt) 1 Antibodies 1 Antibodies 1 Antibodies

General

Antigen Tubulin, beta (TUBB) Antibodies
Epitope N-Term, AA 1-100
(56), (28), (13), (13), (11), (9), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Chinese Hamster, Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(314), (193), (191), (56), (47), (35), (32), (23), (17), (16), (15), (14), (9), (8), (7), (6), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(220), (145), (2), (1)
Clonality
Polyclonal
Conjugate This TUBB antibody is un-conjugated
(13), (8), (6), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(315), (137), (110), (108), (93), (81), (23), (23), (19), (10), (7), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 18 references available
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Product Details anti-TUBB Antibody

Target Details TUBB Application Details Handling References for anti-TUBB Antibody (ABIN269949) Images
Purification Ammonium sulfate precipitation
Immunogen A synthetic peptide made to the N-terminal region of human beta Tubulin (within residues 1-100). [Swiss-Prot P07437]

Target Details TUBB

Product Details anti-TUBB Antibody Application Details Handling References for anti-TUBB Antibody (ABIN269949) Images back to top
Antigen
Alternative Name beta Tubulin (TUBB Antibody Abstract)
Background Gene Symbol: TUBB
Molecular Weight Theoretical MW: 50 kDa
Gene ID 203068, 545486, 498736
UniProt P07437
Research Area Signaling, Cytoskeleton
Pathways Microtubule Dynamics

Application Details

Product Details anti-TUBB Antibody Target Details TUBB Handling References for anti-TUBB Antibody (ABIN269949) Images back to top
Application Notes Western Blot 1:1000, Simple Western 1:1000, Immunohistochemistry 1:1000 - 1:2000, Immunocytochemistry/Immunofluorescence 1:500-1:1000, Immunohistochemistry-Paraffin 1:1000 - 1:2000
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for beta Tubulin Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-beta tubulin primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (we used BioFX Super Plus ECL).Note: Tween-20 can be added to the blocking or antibody diultion buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-TUBB Antibody Target Details TUBB Application Details References for anti-TUBB Antibody (ABIN269949) Images back to top
Format Liquid
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-TUBB Antibody (ABIN269949)

Product Details anti-TUBB Antibody Target Details TUBB Application Details Handling Images back to top
Product cited in:

Chen, Cheng, Tzen, Cheng, Lin: "Probing relevant molecules in modulating the neurite outgrowth of hippocampal neurons on substrates of different stiffness." in: PLoS ONE, Vol. 8, Issue 12, pp. e83394, 2014 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

McKinley, Zhao, Coffey, Washington, Manning: "3'-Deoxy-3'-[18F]-Fluorothymidine PET imaging reflects PI3K-mTOR-mediated pro-survival response to targeted therapy in colorectal cancer." in: PLoS ONE, Vol. 9, Issue 9, pp. e108193, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Jacek, Fallon, Chandra, Crow, Wormser, Alaedini: "Increased IFNα activity and differential antibody response in patients with a history of Lyme disease and persistent cognitive deficits." in: Journal of neuroimmunology, Vol. 255, Issue 1-2, pp. 85-91, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting

McKinley, Smith, Zhao, Fu, Saleh, Uddin, Washington, Coffey, Manning: "3'-Deoxy-3'-18F-fluorothymidine PET predicts response to (V600E)BRAF-targeted therapy in preclinical models of colorectal cancer." in: Journal of nuclear medicine : official publication, Society of Nuclear Medicine, Vol. 54, Issue 3, pp. 424-30, 2013 (Sample species: Human). Further details: Western Blotting

Riva, He, Källberg, Ivars, Leanderson: "Human S100A9 protein is stabilized by inflammatory stimuli via the formation of proteolytically-resistant homodimers." in: PLoS ONE, Vol. 8, Issue 4, pp. e61832, 2013 Further details: Western Blotting

Orlicky, Monks, Stefanski, McManaman: "Dynamics and molecular determinants of cytoplasmic lipid droplet clustering and dispersion." in: PLoS ONE, Vol. 8, Issue 6, pp. e66837, 2013

Lu, Kaliberov, Sohn, Kaliberova, Curiel, Arbeit: "Transcriptional targeting of primary and metastatic tumor neovasculature by an adenoviral type 5 roundabout4 vector in mice." in: PLoS ONE, Vol. 8, Issue 12, pp. e83933, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting

Mikłosz, Łukaszuk, Baranowski, Górski, Chabowski: "Effects of inhibition of serine palmitoyltransferase (SPT) and sphingosine kinase 1 (SphK1) on palmitate induced insulin resistance in L6 myotubes." in: PLoS ONE, Vol. 8, Issue 12, pp. e85547, 2013 (Sample species: Rat (Rattus)). Further details: Western Blotting

Kaucher, Oatley, Oatley: "NEUROG3 is a critical downstream effector for STAT3-regulated differentiation of mammalian stem and progenitor spermatogonia." in: Biology of reproduction, Vol. 86, Issue 5, pp. 164, 1-11, 2012 (Sample species: Mouse (Murine)). Further details: Western Blotting

Li, Zhao, Zhu, Ren, Nie, Gao, Gao, Yang, Zhou, Shen, Wang, Lu, Chen, Hide, Ayala, Lun: "Differences in iNOS and arginase expression and activity in the macrophages of rats are responsible for the resistance against T. gondii infection." in: PLoS ONE, Vol. 7, Issue 4, pp. e35834, 2012 Further details: Western Blotting

Kadiu, Gendelman: "Human immunodeficiency virus type 1 endocytic trafficking through macrophage bridging conduits facilitates spread of infection." in: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, Vol. 6, Issue 4, pp. 658-75, 2011 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Reding, Stepnoski, Cloninger, Oatley: "THY1 is a conserved marker of undifferentiated spermatogonia in the pre-pubertal bull testis." in: Reproduction (Cambridge, England), Vol. 139, Issue 5, pp. 893-903, 2010 (Sample species: Cow (Bovine)). Further details: Western Blotting

Lin, Chen, Sun, Chang, Wang, Yang, Lo: "The protease-mediated nucleus shuttles of subnanometer gold quantum dots for real-time monitoring of apoptotic cell death." in: Journal of the American Chemical Society, Vol. 132, Issue 24, pp. 8309-15, 2010 (Sample species: Human). Further details: Western Blotting

Catalucci, Zhang, DeSantiago, Aimond, Barbara, Chemin, Bonci, Picht, Rusconi, Dalton, Peterson, Richard, Bers, Brown, Condorelli: "Akt regulates L-type Ca2+ channel activity by modulating Cavalpha1 protein stability." in: The Journal of cell biology, Vol. 184, Issue 6, pp. 923-33, 2009

Rattner, Toulabi, Williams, Yu, Nathans: "The genomic response of the retinal pigment epithelium to light damage and retinal detachment." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 39, pp. 9880-9, 2008

Background publications

Southwick, Ernst, Tauriello, Parker, Mujumdar, Mujumdar, Clever, Waggoner: "Cyanine dye labeling reagents--carboxymethylindocyanine succinimidyl esters." in: Cytometry, Vol. 11, Issue 3, pp. 418-30, 1990

Matthes, Wolff, Soubiran, Gros, Dighiero: "Antitubulin antibodies. II. Natural autoantibodies and induced antibodies recognize different epitopes on the tubulin molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 9, pp. 3135-41, 1988

Gozes, Barnstable: "Monoclonal antibodies that recognize discrete forms of tubulin." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, Issue 8, pp. 2579-83, 1982