You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Zebrafish (Danio rerio) TUBB antibody for Immunocytochemistry

Recommended TUBB Antibody (supplied by: Log in to see )

Antigen
Tubulin, beta (TUBB) Antibodies
  • 1t
  • 2t
  • 3t
  • 4t
  • 143391_i_at
  • AA408537
  • AI325297
  • AI596182
  • B1t
  • B2t
  • B3t
  • B4t
  • B130022C14Rik
  • beta
  • beta(2)Tu
  • beta(2)Tub
  • beta-5
  • beta-tub
  • beta-Tub
  • beta-Tub6D
  • beta-Tub56D
  • beta-Tub60D
  • beta-Tub85D
  • beta-tub85D
  • beta-Tub97EF
  • beta-tubulin56D
  • beta1
  • beta1-tub
  • beta1-Tubulin
  • beta1t
  • beta1tub
  • beta1Tub
  • BETA2
  • beta2
  • beta2-tub
  • beta2t
  • beta2tub
  • beta3
  • beta3 TU
  • beta3-Tub
  • beta3-tubulin
  • beta3t
  • beta3TUB
  • beta3Tub
  • beta4
  • beta4t
  • beta56D
  • BETA 56D
  • beta60C
  • BETA 60D
  • BETA 85D
  • beta85D
  • beta97F
  • BETA 98B
  • betaTub
  • betatub(56D)
  • betaTub1
  • betaTub2
  • betaTub3
  • betaTub4
  • betaTub60C
  • betatub60D
  • betaTub98BC
  • betaTub98C
  • beta[[1]]-tubulin
  • beta[[1]] tubulin
  • beta[[2]]-tubulin
  • beta[[3]]-Tub
  • beta[[3]]-tubulin
  • beta[[3]] tubulin
  • bmtub1
  • bmtub2
  • bmtub3
  • bmtub4
  • CG3401
  • CG4869
  • CG9277
  • CG9359
  • D.m.BETA-60D
  • D.m.BETA-85D
  • Dmbeta1
  • Dmbeta2
  • Dmbeta3
  • Dmbeta4
  • Dmel\\CG3401
  • Dmel\\CG4869
  • Dmel\\CG9277
  • Dmel\\CG9359
  • DTB1
  • DTB2
  • DTB3
  • DTB4
  • LOC100101153
  • M(beta)4
  • M(beta)5
  • M40
  • m40
  • ms(3)KK[D]
  • OK/SW-cl.56
  • ok/sw-cl.56
  • p50
  • p50/tubulin
  • p53
  • p53/tubulin
  • T
  • Tub
  • Tub1
  • Tub2
  • Tub3
  • Tub4
  • tub5
  • Tub60D
  • Tubb
  • TUBB
  • TUBB1
  • tubb1
  • TUBB2A
  • tubb2a
  • TUBB2B
  • tubB3
  • tubb4
  • Tubb4
  • tubb5
  • TUBB5
  • tubulin
  • Tubulin
  • XLOT
Epitope
N-Term, AA 1-100
54
28
13
13
11
7
6
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
311
190
188
54
44
32
31
20
17
14
14
12
9
8
7
6
5
5
5
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Host
Rabbit
219
143
2
1
Clonality
Polyclonal
Conjugate
This TUBB antibody is un-conjugated
12
7
6
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
312
136
109
107
93
81
23
23
18
10
7
2
2
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN269949
Contact our Customer Service for availability and price in your country.

Top referenced anti-TUBB antibody for Immunocytochemistry

Similar anti-TUBB Antibodies

Application / Reactivity Pig (Porcine) Mouse (Murine) Human Rat (Rattus) Chicken Xenopus laevis Monkey Hamster Zebrafish (Danio rerio)
Western Blotting (WB) 56 Antibodies 168 Antibodies 273 Antibodies 159 Antibodies 49 Antibodies 16 Antibodies 17 Antibodies 6 Antibodies 18 Antibodies
Immunostaining (ISt) 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Immunoprecipitation (IP) 6 Antibodies 14 Antibodies

General

Antigen Tubulin, beta (TUBB) Antibodies
Epitope N-Term, AA 1-100
(54), (28), (13), (13), (11), (7), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(311), (190), (188), (54), (44), (32), (31), (20), (17), (14), (14), (12), (9), (8), (7), (6), (5), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(219), (143), (2), (1)
Clonality
Polyclonal
Conjugate This TUBB antibody is un-conjugated
(12), (7), (6), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(312), (136), (109), (107), (93), (81), (23), (23), (18), (10), (7), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 18 references available
Supplier Log in to see

Product Details anti-TUBB Antibody

Target Details TUBB Application Details Handling References for anti-TUBB Antibody (ABIN269949) Images
Purification IgG purified
Immunogen A synthetic peptide made to the N-terminal region of human beta Tubulin (within residues 1-100).

Target Details TUBB

Product Details anti-TUBB Antibody Application Details Handling References for anti-TUBB Antibody (ABIN269949) Images back to top
Antigen
Alternative Name beta Tubulin (TUBB Antibody Abstract)
Background Tubulin is the major building block of microtubules. This intracellular cylindricalfilamentous structure is present in almost all eukaryotic cells. Microtubules function asstructural and mobile elements in mitosis, intracellular transport, flagellar movement,and in the cytoskeleton. Antibodies against beta Tubulin are useful as loading controlsfor Western Blotting. Alternate Names: anti-M40 antibody, anti-Tubulin beta polypeptide antibody.
Gene Symbol: TUBB
Gene ID 203068, 545486, 498736
UniProt P07437
Pathways Microtubule Dynamics

Application Details

Product Details anti-TUBB Antibody Target Details TUBB Handling References for anti-TUBB Antibody (ABIN269949) Images back to top
Application Notes This beta Tubulin antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band at ~50 kDa is seen.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:500-1:1000, Western Blot 1:1000
Protocol Protocol specific for beta Tubulin Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-beta tubulin primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (we used BioFX Super Plus ECL).Note: Tween-20 can be added to the blocking or antibody diultion buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-TUBB Antibody Target Details TUBB Application Details References for anti-TUBB Antibody (ABIN269949) Images back to top
Format Liquid
Buffer PBS, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.

References for anti-TUBB Antibody (ABIN269949)

Product Details anti-TUBB Antibody Target Details TUBB Application Details Handling Images back to top
Product cited in:

Chen, Cheng, Tzen, Cheng, Lin: "Probing relevant molecules in modulating the neurite outgrowth of hippocampal neurons on substrates of different stiffness." in: PLoS ONE, Vol. 8, Issue 12, pp. e83394, 2014 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

McKinley, Zhao, Coffey, Washington, Manning: "3'-Deoxy-3'-[18F]-Fluorothymidine PET imaging reflects PI3K-mTOR-mediated pro-survival response to targeted therapy in colorectal cancer." in: PLoS ONE, Vol. 9, Issue 9, pp. e108193, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Jacek, Fallon, Chandra, Crow, Wormser, Alaedini: "Increased IFNα activity and differential antibody response in patients with a history of Lyme disease and persistent cognitive deficits." in: Journal of neuroimmunology, Vol. 255, Issue 1-2, pp. 85-91, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting

McKinley, Smith, Zhao, Fu, Saleh, Uddin, Washington, Coffey, Manning: "3'-Deoxy-3'-18F-fluorothymidine PET predicts response to (V600E)BRAF-targeted therapy in preclinical models of colorectal cancer." in: Journal of nuclear medicine : official publication, Society of Nuclear Medicine, Vol. 54, Issue 3, pp. 424-30, 2013 (Sample species: Human). Further details: Western Blotting

Riva, He, Källberg, Ivars, Leanderson: "Human S100A9 protein is stabilized by inflammatory stimuli via the formation of proteolytically-resistant homodimers." in: PLoS ONE, Vol. 8, Issue 4, pp. e61832, 2013 Further details: Western Blotting

Orlicky, Monks, Stefanski, McManaman: "Dynamics and molecular determinants of cytoplasmic lipid droplet clustering and dispersion." in: PLoS ONE, Vol. 8, Issue 6, pp. e66837, 2013

Lu, Kaliberov, Sohn, Kaliberova, Curiel, Arbeit: "Transcriptional targeting of primary and metastatic tumor neovasculature by an adenoviral type 5 roundabout4 vector in mice." in: PLoS ONE, Vol. 8, Issue 12, pp. e83933, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting

Mikłosz, Łukaszuk, Baranowski, Górski, Chabowski: "Effects of inhibition of serine palmitoyltransferase (SPT) and sphingosine kinase 1 (SphK1) on palmitate induced insulin resistance in L6 myotubes." in: PLoS ONE, Vol. 8, Issue 12, pp. e85547, 2013 (Sample species: Rat (Rattus)). Further details: Western Blotting

Kaucher, Oatley, Oatley: "NEUROG3 is a critical downstream effector for STAT3-regulated differentiation of mammalian stem and progenitor spermatogonia." in: Biology of reproduction, Vol. 86, Issue 5, pp. 164, 1-11, 2012 (Sample species: Mouse (Murine)). Further details: Western Blotting

Li, Zhao, Zhu, Ren, Nie, Gao, Gao, Yang, Zhou, Shen, Wang, Lu, Chen, Hide, Ayala, Lun: "Differences in iNOS and arginase expression and activity in the macrophages of rats are responsible for the resistance against T. gondii infection." in: PLoS ONE, Vol. 7, Issue 4, pp. e35834, 2012 Further details: Western Blotting

Kadiu, Gendelman: "Human immunodeficiency virus type 1 endocytic trafficking through macrophage bridging conduits facilitates spread of infection." in: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, Vol. 6, Issue 4, pp. 658-75, 2011 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Reding, Stepnoski, Cloninger, Oatley: "THY1 is a conserved marker of undifferentiated spermatogonia in the pre-pubertal bull testis." in: Reproduction (Cambridge, England), Vol. 139, Issue 5, pp. 893-903, 2010 (Sample species: Cow (Bovine)). Further details: Western Blotting

Lin, Chen, Sun, Chang, Wang, Yang, Lo: "The protease-mediated nucleus shuttles of subnanometer gold quantum dots for real-time monitoring of apoptotic cell death." in: Journal of the American Chemical Society, Vol. 132, Issue 24, pp. 8309-15, 2010 (Sample species: Human). Further details: Western Blotting

Catalucci, Zhang, DeSantiago, Aimond, Barbara, Chemin, Bonci, Picht, Rusconi, Dalton, Peterson, Richard, Bers, Brown, Condorelli: "Akt regulates L-type Ca2+ channel activity by modulating Cavalpha1 protein stability." in: The Journal of cell biology, Vol. 184, Issue 6, pp. 923-33, 2009

Rattner, Toulabi, Williams, Yu, Nathans: "The genomic response of the retinal pigment epithelium to light damage and retinal detachment." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 39, pp. 9880-9, 2008

Background publications

Southwick, Ernst, Tauriello, Parker, Mujumdar, Mujumdar, Clever, Waggoner: "Cyanine dye labeling reagents--carboxymethylindocyanine succinimidyl esters." in: Cytometry, Vol. 11, Issue 3, pp. 418-30, 1990

Matthes, Wolff, Soubiran, Gros, Dighiero: "Antitubulin antibodies. II. Natural autoantibodies and induced antibodies recognize different epitopes on the tubulin molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 9, pp. 3135-41, 1988

Gozes, Barnstable: "Monoclonal antibodies that recognize discrete forms of tubulin." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, Issue 8, pp. 2579-83, 1982