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|Application / Reactivity||Human||Rat (Rattus)||Mouse (Murine)||Xenopus laevis||Zebrafish (Brachydanio rerio)|
|Immunofluorescence (fixed cells) (IF/ICC)||15 Antibodies||9 Antibodies||11 Antibodies|
|Simple Western (SimWes)||3 Antibodies||3 Antibodies||3 Antibodies||3 Antibodies|
|Western Blotting (WB)||219 Antibodies||312 Antibodies||204 Antibodies||28 Antibodies||2 Antibodies|
|Immunostaining (ISt)||1 Antibodies||1 Antibodies||1 Antibodies|
|Immunoprecipitation (IP)||9 Antibodies||18 Antibodies||16 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||55 Antibodies||77 Antibodies|
|Antigen||Tubulin, beta (TUBB) Antibodies|
|Reactivity||Mouse (Murine), Rat (Rattus), Human, Xenopus laevis, Zebrafish (Brachydanio rerio) Alternatives|
|Conjugate||This TUBB antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-TUBB AntibodyTarget Details TUBB Application Details Handling Images
|Specificity||Specific for Tubulin beta chain.|
|Immunogen||A synthetic peptide from the c-terminal region of human Tubulin beta chain (TUBB5, TUBB2) conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in many other species including rat, mouse, zebrafish and xenopus.|
Target Details TUBBProduct Details anti-TUBB Antibody Application Details Handling Images back to top
|Alternative Name||beta Tubulin (TUBB Antibody Abstract)|
Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Subcellular location: Microtubule
Tissue specificity: Ubiquitously expressed with highest levels in spleen, thymus and immature brain. Also known as: TBB5, Tubulin beta-5 chain, TUBB, TUBB5, Tubulin beta-2A chain, TUBB2A, TUBB2, Tubulin beta-2B chain, Tubulin beta-2C chain.
|Research Area||Signaling, Cytoskeleton|
|Pathways||Microtubule Dynamics, M Phase|
Application DetailsProduct Details anti-TUBB Antibody Target Details TUBB Handling Images back to top
A dilution of 1: 300 to 1: 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TUBB Antibody Target Details TUBB Application Details Images back to top
|Reconstitution||Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.|
|Handling Advice||Avoid freeze and thaw cycles.|
|Storage||4 °C/-20 °C|
|Storage Comment||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Expiry Date||12 months|