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anti-Mouse (Murine) TUBB1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended TUBB1 Antibody (supplied by: Log in to see )

Antigen
Tubulin, beta 1 (TUBB1) Antibodies
  • 2810484G07Rik
  • M(beta)1
  • TUBB1
  • TUBB3
Epitope
AA 182-437
8
8
6
4
3
1
1
1
Reactivity
Mouse (Murine)
47
12
11
4
1
1
Host
Rabbit
50
13
Clonality
Polyclonal
Conjugate
This TUBB1 antibody is un-conjugated
7
7
6
2
2
2
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
50
27
9
9
4
3
2
1
1
1
Supplier
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Catalog No. ABIN1860884
$ 396.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089690165 ABIN265867 ICC IF IHC (p) IHC ELISA WB Mouse IgG1 C-Term Log in to see SAP-4G5

Top referenced anti-TUBB1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-TUBB1 Antibodies

Application / Reactivity Mouse (Murine)
ELISA 4 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 3 Antibodies
Immunohistochemistry (IHC) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Western Blotting (WB) 10 Antibodies

General

Antigen Tubulin, beta 1 (TUBB1) Antibodies
Epitope AA 182-437
(8), (8), (6), (4), (3), (1), (1), (1)
Reactivity Mouse (Murine)
(47), (12), (11), (4), (1), (1)
Host Rabbit
(50), (13)
Clonality
Polyclonal
Conjugate This TUBB1 antibody is un-conjugated
(7), (7), (6), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(50), (27), (9), (9), (4), (3), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-TUBB1 Antibody

Target Details TUBB1 Application Details Handling References for anti-TUBB1 Antibody (ABIN1860884) Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-PYNAVLSIH QLIENTDACF CIDNEALYDI CFRTLRLTTP TYGDLNHLVS LTMSGITTSL RFPGQLNADL RKLAVNMVPF PRLHFFMPGF APLTAQGSQQ YRALSVAELT QQMFDARNIM AACDPRRGRY LTVACIFRGK MSTKEVDQQL LSIQTRNSNC FVEWIPNNVK VAVCDIPPRG LNMAATFLGN NTAIQELFTR VSEHFSAMFR RRAFVHWYTS EGMDISEFGE AESDIHDLVS EYQQFQDVRA GLEDSEE
Specificity It has been selected for its ability to recognize TUBb1 in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen TUBb1 (AA 182-437)
Isotype IgG

Target Details TUBB1

Product Details anti-TUBB1 Antibody Application Details Handling References for anti-TUBB1 Antibody (ABIN1860884) Images back to top
Antigen
Alternative Name Tubulin Beta 1 (TUBb1) (TUBB1 Antibody Abstract)
Pathways Microtubule Dynamics

Application Details

Product Details anti-TUBB1 Antibody Target Details TUBB1 Handling References for anti-TUBB1 Antibody (ABIN1860884) Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant TUBb1 (Pro182~Glu437) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-TUBB1 Antibody Target Details TUBB1 Application Details References for anti-TUBB1 Antibody (ABIN1860884) Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

References for anti-TUBB1 Antibody (ABIN1860884)

Product Details anti-TUBB1 Antibody Target Details TUBB1 Application Details Handling Images back to top
Product cited in:

Zhao, Li, Han, Yue, Shi, Wang, Guo, Lu: "DDAH1 deficiency promotes intracellular oxidative stress and cell apoptosis via a miR-21-dependent pathway in mouse embryonic fibroblasts." in: Free radical biology & medicine, Vol. 92, pp. 50-60, 2016

Images

Product Details anti-TUBB1 Antibody Target Details TUBB1 Application Details Handling References for anti-TUBB1 Antibody (ABIN1860884) back to top
Supplier Images
Western Blotting (WB) image for anti-TUBB1 antibody (Tubulin, beta 1) (AA 182-437) (ABIN1860884) anti-Tubulin, beta 1 (TUBB1) (AA 182-437) antibody