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|Application / Reactivity||Human||Chicken||Mouse (Murine)||Rat (Rattus)||Cow (Bovine)||Cynomolgus|
|Western Blotting (WB)||158 Antibodies||2 Antibodies||45 Antibodies||50 Antibodies||8 Antibodies||1 Antibodies|
|Intracellular Flow Cytometry (ICFC)||2 Antibodies||2 Antibodies||2 Antibodies|
|Immunoprecipitation (IP)||18 Antibodies||7 Antibodies||7 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||1 Antibodies||4 Antibodies||29 Antibodies||13 Antibodies||18 Antibodies|
|Immunohistochemistry (IHC)||76 Antibodies||23 Antibodies|
|Antigen||Tubulin, Beta, 3 (TUBB3) Antibodies|
|Epitope||AA 150-200 Alternatives|
|Reactivity||Cynomolgus, Cow (Bovine), Chicken, Human, Rat (Rattus), Mouse (Murine) Alternatives|
|Conjugate||This TUBB3 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
|4 references available|
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Product Details anti-TUBB3 AntibodyTarget Details TUBB3 Application Details Handling References for anti-TUBB3 Antibody (ABIN1574042) Images
|Specificity||Rabbit Anti-beta-Tubulin III Polyclonal Antibody detects endogenous levels of human, rat and mouse beta-tubulin III protein. Sequence homology predicts that it will also react with bovine, chicken, cynomolgus monkey. African clawed frog, and disc abalone beta-tubulin III proteins. Reactivity with other β-Tubulin had not been tested yet.|
|Cross-Reactivity (Details)||Rabbit Anti-beta-Tubulin III Polyclonal Antibody detects endogenous levels of human, rat and mouse beta-tubulin III protein. Sequence homology predicts that it will also react with bovine, chicken, cynomolgus monkey. African clawed frog, and disc abalone beta-tubulin III proteins.|
|Immunogen||Synthetic peptide (KLH-coupled) within AA 150-200 of human β-tubulin III protein|
Target Details TUBB3Product Details anti-TUBB3 Antibody Application Details Handling References for anti-TUBB3 Antibody (ABIN1574042) Images back to top
|Alternative Name||beta-Tubulin III (TUBB3 Antibody Abstract)|
|Background||Beta-Tubulin III belongs to the tubulin family. Tubulin is the major constituent of microtubules. It forms dimers of alpha and beta chains. One mole of tubulin binds to two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. beta-Tubulin III contains a highly acidic C-terminal region which can bind cations such as calcium. It is abundant in the central and peripheral nervous systems.Rabbit Anti-beta-Tubulin III Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide within residues 150-200 of human beta-Tubulin III protein (Swiss Prot: Q13509).|
Application DetailsProduct Details anti-TUBB3 Antibody Target Details TUBB3 Handling References for anti-TUBB3 Antibody (ABIN1574042) Images back to top
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL. Western blot: 0.5-1 µg/mL. Immunohistochemistry: 5-10 µg/mL. Flow cytometry: 1-3 µg for 1 x 10E6 cells. Other applications: user-optimized.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TUBB3 Antibody Target Details TUBB3 Application Details References for anti-TUBB3 Antibody (ABIN1574042) Images back to top
|Buffer||PBS, pH 7.4, containing 0.02 % sodium azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Storage||4 °C/-20 °C|
|Storage Comment||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
References for anti-TUBB3 Antibody (ABIN1574042)Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling Images back to top
|Product cited in:||
Hasegawa, Ryu, Kaláb: "Chromosomal gain promotes formation of a steep RanGTP gradient that drives mitosis in aneuploid cells." in: The Journal of cell biology, Vol. 200, Issue 2, pp. 151-61, 2013 (PubMed).
Yang, Zhu, Chen et al.: "Inhibition of farnesyl pyrophosphate synthase attenuates angiotensin II-induced cardiac hypertrophy and fibrosis in vivo." in: The international journal of biochemistry & cell biology, Vol. 45, Issue 3, pp. 657-66, 2013 (PubMed).
Das, Anczuków, Akerman et al.: "Oncogenic splicing factor SRSF1 is a critical transcriptional target of MYC." in: Cell reports, Vol. 1, Issue 2, pp. 110-7, 2012 (PubMed).
Hua, Sahashi, Hung et al.: "Antisense correction of SMN2 splicing in the CNS rescues necrosis in a type III SMA mouse model." in: Genes & development, Vol. 24, Issue 15, pp. 1634-44, 2010 (PubMed).
ImagesProduct Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling References for anti-TUBB3 Antibody (ABIN1574042) back to top