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|Antigen||Tubulin, Beta, 3 (TUBB3) Antibodies|
|Conjugate||This TUBB3 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|14 references available|
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Product Details anti-TUBB3 AntibodyTarget Details TUBB3 Application Details Handling References for anti-TUBB3 Antibody (ABIN93911) Images
|Specificity||The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.|
|Purification||Purified by ammonium sulphate and caprylic acid precipitation|
|Purity||> 95 % (by SDS-PAGE)|
|Immunogen||Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.|
Target Details TUBB3Product Details anti-TUBB3 Antibody Application Details Handling References for anti-TUBB3 Antibody (ABIN93911) Images back to top
|Alternative Name||betaIII-tubulin (TUBB3 Antibody Abstract)|
|Background||The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.|
Application DetailsProduct Details anti-TUBB3 Antibody Target Details TUBB3 Handling References for anti-TUBB3 Antibody (ABIN93911) Images back to top
Western Blotting: Recommended dilution: 1-2 µg/mL, 90 min
Positive control: Porcine brain lysate
Negative control: HPB-ALL human peripheral blood leukemia cell line
Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
Application note: Reducing conditions.
Immunohistochemistry (paraffin sections) Recommended dilution: 10 µg/mL
Staining technique: Standard ABC technique (DAB+)
Pretreatment: 0.1 % pepsin (trypsin) in 0.1 M HCl,
Incubation 30 min in RT, or High temperature citrate buffer antigen retrieval
Positive tissue: neuronal tissueImmunocytochemistry
Positive material: Neuro2a mouse neuroblastoma cell line
Working concentrations should be determined by the investigator.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TUBB3 Antibody Target Details TUBB3 Application Details References for anti-TUBB3 Antibody (ABIN93911) Images back to top
|Buffer||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Do not freeze.|
|Storage Comment||Store at 2-8 °C. Do not use after expiration date stamped on vial label.|
References for anti-TUBB3 Antibody (ABIN93911)Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling Images back to top
|Product cited in:||
Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, 2012 (PubMed).
Gun?ova: "The neurodegenerative process in a neurotoxic rat model and in patients with Huntington's disease: Histopathological parallels and differences." in: Acta histochemica, 2011 (PubMed).
Kunova, Matulka, Eiselleova et al.: "Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells." in: Reproductive biomedicine online, Vol. 21, Issue 5, pp. 676-86, 2010 (PubMed).
Jirásek, Cipro, Musilová et al.: "Expression of class III beta-tubulin in colorectal carcinomas: an immunohistochemical study using TU-20 & TuJ-1 antibody." in: The Indian journal of medical research, Vol. 129, Issue 1, pp. 89-94, 2009 (PubMed).
Theodorou, Dalembert, Heffelfinger et al.: "A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation." in: Genes & development, Vol. 23, Issue 5, pp. 575-88, 2009 (PubMed).
Dráberová, Del Valle, Gordon et al.: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, 2008 (PubMed).
Katsetos, Dráberová, Smejkalová et al.: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, 2007 (PubMed).
Jirásek, Písaríková, Viklický et al.: "Expression of class III beta-tubulin in malignant epithelial tumours: an immunohistochemical study using TU-20 and TuJ-1 antibodies." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 45, Issue 1, pp. 41-5, 2007 (PubMed).
Kukharskyy, Sulimenko, Mac?rek et al.: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, 2004 (PubMed).
P?knicová, Kubátová, Sulimenko et al.: "Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail." in: Biology of reproduction, Vol. 65, Issue 3, pp. 672-9, 2001 (PubMed).
Zíková, Sulimenko, Dráber et al.: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, 2000 (PubMed).
Dráberová, Lukás, Ivanyi et al.: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, 1998 (PubMed).
Silva, Pereira, Oliveira et al.: "BDNF and extracellular matrix regulate differentiation of mice neurosphere-derived cells into a GABAergic neuronal phenotype." in: Journal of neuroscience research, Vol. 87, Issue 9, pp. 1986-96, 2009 (PubMed).
Woltjen, Michael, Mohseni et al.: "piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells." in: Nature, Vol. 458, Issue 7239, pp. 766-70, 2009 (PubMed).
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