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anti-Human TUBB3 antibody for Flow Cytometry

Recommended TUBB3 Antibody (supplied by: Log in to see )

Antigen
Tubulin, Beta, 3 (TUBB3) Antibodies
  • 3200002H15Rik
  • AI325297
  • beta-4
  • CDCBM
  • CFEOM3A
  • M(beta)3
  • M(beta)4
  • M(beta)6
  • Tubb
  • tubb3
  • TUBB3
  • Tubb4
  • TUBB4
Reactivity
Human
188
55
52
11
4
4
4
2
2
2
1
1
Host
Mouse
101
89
14
12
1
Clonality
Monoclonal
Conjugate
This TUBB3 antibody is un-conjugated
23
12
11
3
3
3
3
2
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
177
80
79
43
42
35
33
16
13
9
6
2
2
2
Supplier
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Catalog No. ABIN969447
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN2657231 FACS Alexa Fluor 647 Mouse IgG2a Log in to see AA10 1
0.30226976 ABIN2500819 FACS IF ELISA WB Mouse IgG1 Log in to see 2000000000
0.30226976 ABIN1846608 ICC FACS IF ELISA WB Mouse IgG1 Log in to see
0.0010482287 ABIN651938 FACS IHC (p) WB Rabbit Ig AA 36-63, N-Term Log in to see Polyclonal 1
0.0010482287 ABIN574424 ICC FACS IHC (p) WB Mouse IgG1 C-Term Log in to see TU-20 6
0.0010482287 ABIN1574042 FACS IHC ELISA WB Rabbit IgG AA 150-200 Log in to see Polyclonal 4
0.0010482287 ABIN258508 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see EP1331Y
0.0010482287 ABIN466706 FACS IF ELISA WB Mouse IgG1 Log in to see 2E9
0.0010482287 ABIN258509 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see EP1569Y
0.0010482287 ABIN967213 FACS IF ELISA WB Mouse IgG1 Log in to see 2E9 2
0.0010482287 ABIN1945703 FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG N-Term, AA 43-71 Log in to see Polyclonal
0.0010482287 ABIN1945704 FACS IHC ELISA WB APC Rabbit IgG N-Term, AA 43-71 Log in to see Polyclonal
0.0010482287 ABIN1945705 FACS IHC ELISA WB PE Rabbit IgG N-Term, AA 43-71 Log in to see Polyclonal
0.0010482287 ABIN1838603 FACS IHC (p) ELISA WB Rabbit AA 150-200 Log in to see Polyclonal
0.0010482287 ABIN1724161 ICC FACS IHC (p) WB Mouse IgG1 N-Term Log in to see TU-20
0.0010482287 ABIN2657232 FACS Alexa Fluor 647 Mouse IgG2a Log in to see AA10 1
0.0010482287 ABIN3021273 ICC FACS IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0010482287 ABIN2576128 FACS Rabbit IgG AA 43-71 Log in to see Polyclonal
0.0010482287 ABIN1839149 FACS IHC WB Rabbit N-Term, AA 36-63 Log in to see Polyclonal
0.0010482287 ABIN2737781 ICC FACS IP IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-TUBB3 antibody for Flow Cytometry

Similar anti-TUBB3 Antibodies

Application / Reactivity Human
ELISA 81 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 38 Antibodies
Immunochromatography (IC) 7 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 29 Antibodies
Immunohistochemistry (IHC) 75 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies
Immunoprecipitation (IP) 18 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Western Blotting (WB) 157 Antibodies

General

Antigen Tubulin, Beta, 3 (TUBB3) Antibodies
Reactivity Human
(188), (55), (52), (11), (4), (4), (4), (2), (2), (2), (1), (1)
Host Mouse
(101), (89), (14), (12), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TUBB3 antibody is un-conjugated
(23), (12), (11), (3), (3), (3), (3), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(177), (80), (79), (43), (42), (35), (33), (16), (13), (9), (6), (2), (2), (2)
Pubmed 2 references available
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Product Details anti-TUBB3 Antibody

Target Details TUBB3 Application Details Handling References for anti-TUBB3 Antibody (ABIN969447) Images
Purification Ascites
Immunogen Purified recombinant fragment of human TUBB3 expressed in E. coli.
Clone 2-00E-09
Isotype IgG1

Target Details TUBB3

Product Details anti-TUBB3 Antibody Application Details Handling References for anti-TUBB3 Antibody (ABIN969447) Images back to top
Antigen
Alternative Name TUBB3 (TUBB3 Antibody Abstract)
Background Tubulin, beta 3, also known as TUBB3. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non exchangeable site on the alpha-chain. Tubulin is a highly conserved protein with a molecular weight of ~50 kD. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. The two main tubulin isoforms, α- and β-tubulin, are usually products of separate genes. The β-tubulin family includes six expressed genes that produce the polypeptide isoforms known as Classes I through VI, each of which have a distinct pattern of expression. Class III β-tubulin is found in neurons and mammalian testis cells and is widely used as a neuronal marker in developmental neurobiology, neoplasia, and stem cell research. Class III β-tubulin expression in neuronal and neuroblastic tumors is differentiation dependent, and its expression in certain non-neuronal neoplasms has been associated with poor prognosis and/or resistance to chemotherapy.
Molecular Weight 50 kDa
Gene ID 10381
HGNC 10381
Pathways Microtubule Dynamics

Application Details

Product Details anti-TUBB3 Antibody Target Details TUBB3 Handling References for anti-TUBB3 Antibody (ABIN969447) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details References for anti-TUBB3 Antibody (ABIN969447) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-TUBB3 Antibody (ABIN969447)

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling Images back to top
Product cited in:

Katsetos, Dráberová, Smejkalová, Reddy, Bertrand, de Chadarévian, Legido, Nissanov, Baas, Dráber: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, 2007

Background publications

Vinores, Derevjanik, Mahlow, Hackett, Haller, deJuan, Frankfurter, Campochiaro: "Class III beta-tubulin in human retinal pigment epithelial cells in culture and in epiretinal membranes." in: Experimental eye research, Vol. 60, Issue 4, pp. 385-400, 1995

Images

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling References for anti-TUBB3 Antibody (ABIN969447) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (ABIN969447) Figure 1: Flow cytometric analysis of A549 cells using TUBB3 mouse mAb (green) and ne...
Immunocytochemistry (ICC) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (ABIN969447) Figure 2: Immunofluorescence analysis of PANC-1 cells using TUBB3 mouse mAb (green) ....
Western Blotting (WB) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (ABIN969447) Figure 3: Western blot analysis using TUBB3 mouse mAb against HepG2 (1), A549 (2) and...